| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Journal of Lipid Research, Vol 33, 1876-1881, Copyright © 1992 by Lipid Research, Inc.
DL Rainwater, DW Andres, AL Ford, F Lowe, PJ Blanche and RM Krauss
We describe a protocol to cast nondenaturing polyacrylamide gradient gels
(SFBR3/31) for the size resolution of lipoproteins. The protocol yields
gels with minimal lot-to-lot variation in length and electrophoretic
properties. Absorbance profiles of cholesterol-stained lipoproteins in
baboon sera were used to estimate the relative amounts of stain in four
lipoprotein size classes (VLDL+LDL, HDL1, HDL2, and HDL3). When compared
with gels from a commercial source, the SFBR3/31 gels gave very similar
results in terms of precision (coefficients of variation) and of estimated
amounts of lipoproteins in the four size classes. In other studies, we
estimated peak diameters of protein- stained human lipoproteins after
calibrating the gels with size standards. Peak diameters estimated using
SFBR3/31 gels were highly correlated (r2 = 0.99, n = 33) with those
estimated using gels from a commercial source. We conclude that the
protocol reliably produces gradient gels that are suitable for the analysis
of lipoprotein phenotypes.
ARTICLES
Production of polyacrylamide gradient gels for the electrophoretic resolution of lipoproteins [published erratum appears in J Lipid Res 1993 Mar;34(3):520]
Department of Genetics, Southwest Foundation for Biomedical Research, San Antonio, TX 78228.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. P. Caulfield, S. Li, G. Lee, P. J. Blanche, W. A. Salameh, W. H. Benner, R. E. Reitz, and R. M. Krauss Direct Determination of Lipoprotein Particle Sizes and Concentrations by Ion Mobility Analysis Clin. Chem., August 1, 2008; 54(8): 1307 - 1316. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. I Katcher, R. S Legro, A. R Kunselman, P. J Gillies, L. M Demers, D. M Bagshaw, and P. M Kris-Etherton The effects of a whole grain enriched hypocaloric diet on cardiovascular disease risk factors in men and women with metabolic syndrome Am. J. Clinical Nutrition, January 1, 2008; 87(1): 79 - 90. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Skropeta, C. Settasatian, M. R. McMahon, K. Shearston, D. Caiazza, K. C. McGrath, W. Jin, D. J. Rader, P. J. Barter, and K.-A. Rye N-Glycosylation regulates endothelial lipase-mediated phospholipid hydrolysis in apoE- and apoA-I-containing high density lipoproteins J. Lipid Res., September 1, 2007; 48(9): 2047 - 2057. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-A. Rye, R. Bright, M. Psaltis, and P. J. Barter Regulation of reconstituted high density lipoprotein structure and remodeling by apolipoprotein E J. Lipid Res., May 1, 2006; 47(5): 1025 - 1036. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. J. Hime, K. J. Drew, K. Wee, P. J. Barter, and K.-A. Rye Formation of high density lipoproteins containing both apolipoprotein A-I and A-II in the rabbit J. Lipid Res., January 1, 2006; 47(1): 115 - 122. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-Y. Lee, J. M. Timmins, A. Mulya, T. L. Smith, Y. Zhu, E. M. Rubin, J. W. Chisholm, P. L. Colvin, and J. S. Parks HDLs in apoA-I transgenic Abca1 knockout mice are remodeled normally in plasma but are hypercatabolized by the kidney J. Lipid Res., October 1, 2005; 46(10): 2233 - 2245. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Jahangiri, D. J. Rader, D. Marchadier, L. K. Curtiss, D. J. Bonnet, and K-A. Rye Evidence that endothelial lipase remodels high density lipoproteins without mediating the dissociation of apolipoprotein A-I J. Lipid Res., May 1, 2005; 46(5): 896 - 903. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Carmena, P. Duriez, and J.-C. Fruchart Atherogenic Lipoprotein Particles in Atherosclerosis Circulation, June 15, 2004; 109(23_suppl_1): III-2 - III-7. [Abstract] [Full Text]
|
|
|
|
|
|
D. L. Rainwater, P. H. Moore Jr., and I. O. Gamboa Improved method for making nondenaturing composite gradient gels for the electrophoretic separation of lipoproteins J. Lipid Res., April 1, 2004; 45(4): 773 - 775. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Rizzo, J. M. Taylor, C. M. Barbagallo, K. Berneis, P. J. Blanche, and R. M. Krauss Effects on Lipoprotein Subclasses of Combined Expression of Human Hepatic Lipase and Human apoB in Transgenic Rabbits Arterioscler. Thromb. Vasc. Biol., January 1, 2004; 24(1): 141 - 146. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Austin, K. L. Edwards, S. A. Monks, K. M. Koprowicz, J. D. Brunzell, A. G. Motulsky, M. C. Mahaney, and J. E. Hixson Genome-wide scan for quantitative trait loci influencing LDL size and plasma triglyceride in familial hypertriglyceridemia J. Lipid Res., November 1, 2003; 44(11): 2161 - 2168. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Berneis, M. La Belle, P. J. Blanche, and R. M. Krauss Analysis and quantitation of biotinylated apoB-containing lipoproteins with streptavidin-Cy3 J. Lipid Res., July 1, 2002; 43(7): 1155 - 1159. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. Furbee Jr., O. Francone, and J. S. Parks In vivo contribution of LCAT to apolipoprotein B lipoprotein cholesteryl esters in LDL receptor and apolipoprotein E knockout mice J. Lipid Res., March 1, 2002; 43(3): 428 - 437. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. Chisholm, E. R. Burleson, G. S. Shelness, and J. S. Parks ApoA-I secretion from HepG2 cells: evidence for the secretion of both lipid-poor apoA-I and intracellularly assembled nascent HDL J. Lipid Res., January 1, 2002; 43(1): 36 - 44. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. Furbee Jr., O. Francone, and J. S. Parks Alteration of plasma HDL cholesteryl ester composition with transgenic expression of a point mutation (E149A) of human LCAT J. Lipid Res., October 1, 2001; 42(10): 1626 - 1635. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. G. Sorci-Thomas, M. Thomas, L. Curtiss, and M. Landrum Single Repeat Deletion in ApoA-I Blocks Cholesterol Esterification and Results in Rapid Catabolism of Delta 6 and Wild-type ApoA-I in Transgenic Mice J. Biol. Chem., April 14, 2000; 275(16): 12156 - 12163. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Clay, D. H. Pyle, K.-A. Rye, and P. J. Barter Formation of Spherical, Reconstituted High Density Lipoproteins Containing Both Apolipoproteins A-I and A-II Is Mediated by Lecithin:Cholesterol Acyltransferase J. Biol. Chem., March 17, 2000; 275(12): 9019 - 9025. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Schlenck, B. Herbeth, G. Siest, and S. Visvikis Characterization and quantification of serum lipoprotein subfractions by capillary isotachophoresis: relationships with lipid, apolipoprotein, and lipoprotein levels J. Lipid Res., November 1, 1999; 40(11): 2125 - 2133. [Abstract] [Full Text]
|
|
|
|
 |
|
 L. F. Tinker, E. J Parks, S. R. Behr, B. O. Schneeman, and P. A. Davis (n-3) Fatty Acid Supplementation in Moderately Hypertriglyceridemic Adults Changes Postprandial Lipid and Apolipoprotein B Responses to a Standardized Test Meal J. Nutr., June 1, 1999; 129(6): 1126 - 1134. [Abstract] [Full Text]
|
|
|
|
|
|
C. M. Barbagallo, J. Fan, P. J. Blanche, M. Rizzo, J. M. Taylor, and R. M. Krauss Overexpression of Human Hepatic Lipase and ApoE in Transgenic Rabbits Attenuates Response to Dietary Cholesterol and Alters Lipoprotein Subclass Distributions Arterioscler. Thromb. Vasc. Biol., March 1, 1999; 19(3): 625 - 632. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. W. Huggins, L. K. Curtiss, A. K. Gebre, and J. S. Parks Effect of long chain polyunsaturated fatty acids in the sn-2 position of phosphatidylcholine on the interaction with recombinant high density lipoprotein apolipoprotein A-I J. Lipid Res., December 1, 1998; 39(12): 2423 - 2431. [Abstract] [Full Text]
|
|
|
|
|
|
S. Qiu, N. Bergeron, L. Kotite, R. M. Krauss, A. Bensadoun, and R. J. Havel Metabolism of lipoproteins containing apolipoprotein B in hepatic lipase-deficient mice J. Lipid Res., August 1, 1998; 39(8): 1661 - 1668. [Abstract] [Full Text]
|
|
|
|
|
|
M. G. Sorci-Thomas, L. Curtiss, J. S. Parks, M. J. Thomas, M. W. Kearns, and M. Landrum The Hydrophobic Face Orientation of Apolipoprotein A-I Amphipathic Helix Domain 143-164 Regulates Lecithin:Cholesterol Acyltransferase Activation J. Biol. Chem., May 8, 1998; 273(19): 11776 - 11782. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. L. Rainwater, B. D. Mitchell, M. C. Mahaney, and S. M. Haffner Genetic Relationship Between Measures of HDL Phenotypes and Insulin Concentrations Arterioscler. Thromb. Vasc. Biol., December 1, 1997; 17(12): 3414 - 3419. [Abstract] [Full Text]
|
|
|
|
|
|
L. Mykkanen, S. M. Haffner, D. L. Rainwater, P. Karhapaa, H. Miettinen, and M. Laakso Relationship of LDL Size to Insulin Sensitivity in Normoglycemic Men Arterioscler. Thromb. Vasc. Biol., July 1, 1997; 17(7): 1447 - 1453. [Abstract] [Full Text]
|
|
|
|
|
|
A. G. Comuzzie, D. L. Rainwater, J. Blangero, M. C. Mahaney, J. L. VandeBerg, and J. W. MacCluer Shared and Unique Genetic Effects Among Seven HDL Phenotypes Arterioscler. Thromb. Vasc. Biol., May 1, 1997; 17(5): 859 - 864. [Abstract] [Full Text]
|
|
|
|
|
|
M. G. Sorci-Thomas, L. Curtiss, J. S. Parks, M. J. Thomas, and M. W. Kearns Alteration in Apolipoprotein A-I 22-Mer Repeat Order Results in a Decrease in Lecithin:Cholesterol Acyltransferase Reactivity J. Biol. Chem., March 14, 1997; 272(11): 7278 - 7284. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. T.K. Singh, D. L. Rainwater, C. M. Kammerer, R. M. Sharp, M. Poushesh, W. R. Shelledy, and J. L. VandeBerg Dietary and Genetic Effects on LDL Size Measures in Baboons Arterioscler. Thromb. Vasc. Biol., December 1, 1996; 16(12): 1448 - 1453. [Abstract] [Full Text]
|
|
|
|
|
|
E. L. Gong, L. J. Stoltzfus, C. M. Brion, D. Murugesh, and E. M. Rubin Contrasting in Vivo Effects of Murine and Human Apolipoprotein A-II J. Biol. Chem., March 15, 1996; 271(11): 5984 - 5987. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. T.K. Singh, D. L. Rainwater, S. M. Haffner, J. L. VandeBerg, W. R. Shelledy, P. H. Moore Jr, and T. D. Dyer Effect of Diabetes on Lipoprotein Size Arterioscler. Thromb. Vasc. Biol., November 1, 1995; 15(11): 1805 - 1811. [Abstract] [Full Text]
|
|
|
|
|
|
M. A. Austin, L. Mykkanen, J. Kuusisto, K. L. Edwards, C. Nelson, S. M. Haffner, K. Pyorala, and M. Laakso Prospective Study of Small LDLs as a Risk Factor for Non–Insulin Dependent Diabetes Mellitus in Elderly Men and Women Circulation, October 1, 1995; 92(7): 1770 - 1778. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |
