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Journal of Lipid Research, Vol 33, 1876-1881, Copyright © 1992 by Lipid Research, Inc.
DL Rainwater, DW Andres, AL Ford, F Lowe, PJ Blanche and RM Krauss
We describe a protocol to cast nondenaturing polyacrylamide gradient gels
(SFBR3/31) for the size resolution of lipoproteins. The protocol yields
gels with minimal lot-to-lot variation in length and electrophoretic
properties. Absorbance profiles of cholesterol-stained lipoproteins in
baboon sera were used to estimate the relative amounts of stain in four
lipoprotein size classes (VLDL+LDL, HDL1, HDL2, and HDL3). When compared
with gels from a commercial source, the SFBR3/31 gels gave very similar
results in terms of precision (coefficients of variation) and of estimated
amounts of lipoproteins in the four size classes. In other studies, we
estimated peak diameters of protein- stained human lipoproteins after
calibrating the gels with size standards. Peak diameters estimated using
SFBR3/31 gels were highly correlated (r2 = 0.99, n = 33) with those
estimated using gels from a commercial source. We conclude that the
protocol reliably produces gradient gels that are suitable for the analysis
of lipoprotein phenotypes.
ARTICLES
Production of polyacrylamide gradient gels for the electrophoretic resolution of lipoproteins [published erratum appears in J Lipid Res 1993 Mar;34(3):520]
Department of Genetics, Southwest Foundation for Biomedical Research, San Antonio, TX 78228.
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