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Journal of Lipid Research, Vol 33, 241-249, Copyright © 1992 by Lipid Research, Inc.
B Saffari, JM Ong and PA Kern
Lipoprotein lipase (LPL) is an important enzyme in lipid metabolism, and
adipose LPL activity is increased in rats that are deficient in thyroid
hormone. To examine the mechanism of thyroid hormone's effect on LPL, LPL
gene expression was assessed in the epididymal fat pads of hypothyroid
rats. When compared to control rats, LPL activity, mass, and synthetic rate
in hypothyroid rats were increased; heparin- releasable LPL activity and
mass were increased to 448% and 300% of control, respectively, and
[35S]methionine incorporation into LPL was increased to 250% of control.
The increases in LPL activity and mass were reversed by treatment of
hypothyroid rats with triiodothyronine (T3). However, there was no change
in the level of LPL mRNA when compared to the level of gamma-actin mRNA and
no effect on LPL transcription using run-off assays. Isolated adipocytes
were prepared from normal rats and exposed to 2 nM T3 in vitro for 24 h.
The addition of T3 to cultures of adipocytes resulted in a decrease in LPL
activity, mass, and [35S]methionine incorporation, but still no change in
LPL mRNA level. To determine whether thyroid hormone regulated
catecholamine responsiveness, adipocytes were prepared from hypothyroid and
control rats, and the responses to epinephrine were compared. Although
epinephrine inhibited [35S]methionine incorporation into LPL in control rat
adipocytes, there was essentially no effect in hypothyroid rat cells. In
addition, T3 treatment of the hypothyroid rats restored the responsiveness
to epinephrine. Thus, thyroid hormone regulates LPL in rat adipose tissue
posttranscriptionally, resulting in parallel changes in LPL synthetic rate,
immunoreactive mass, and activity.(ABSTRACT TRUNCATED AT 250 WORDS)
ARTICLES
Regulation of adipose tissue lipoprotein lipase gene expression by thyroid hormone in rats
Department of Medicine, Cedars-Sinai Medical Center, Los Angeles, CA 90048.
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