Journal of Lipid Research, Vol 33, 323-331, Copyright © 1992 by Lipid Research, Inc.

ARTICLES

Metabolism of oxygenated derivatives of arachidonic acid by Caco-2 cells

TE Riehl, J Turk and WF Stenson
Department of Gastroenterology, Jewish Hospital of St. Louis, MO.

Monolayers of Caco-2 cells, a human enterocyte cell line, were incubated separately with 3H8-labeled preparations of three different lipid mediators of inflammation: 5-hydroxyeicosatetraenoic acid, 12- hydroxyeicosatetraenoic acid, and leukotriene B4. Both [3H8]5- hydroxyeicosatetraenoic and [3H8]12-hydroxyeicosatetraenoic acids were taken up and metabolized by Caco-2 cells, but [3H]leukotriene B4 remained unmetabolized in the incubation medium. [3H]5- hydroxyeicosatetraenoic acid was esterified into cellular phospholipids (15%) and triglycerides (4%) but did not undergo beta-oxidation. When [3H]12-hydroxyeicosatetraenoic acid was incubated with Caco-2 cells, 14% underwent two cycles of beta-oxidation to form [3H]8- hydroxyhexadecatrienoic acid, and 3% underwent three cycles of beta- oxidation to form [3H]6-hydroxytetradecadienoic acid, both of which were released into the media. [3H]12-Hydroxyeicosatetraenoic acid was also esterified into cellular phospholipids (13%), but none was esterified into cellular triglycerides.
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