Journal of Lipid Research, Vol 33, 431-439, Copyright © 1992 by Lipid Research, Inc.
Quantitation of model digestive mixtures by 13C NMR
D Wang, NL Hadipour, EA Jerlin and RE Stark
Department of Chemistry, College of Staten Island, City University of New York 10301.
13C nuclear magnetic resonance (NMR) spectra were obtained at 50.3 and
100.5 MHz for methanolic and aqueous mixtures of sodium taurocholate, 1-
monocapryloyl-rac-glycerol, and caprylic acid. Distortionless Enhancement
by Polarization Transfer (DEPT) was used to improve spectral sensitivity
and resolution, and to generate calibration curves for quantitative
determinations of each lipid in methanol. Alternatively, the heights for
nonoverlapping peaks in a 13C NMR spectrum acquired with inverse-gated
decoupling provide reliable quantitative estimates for each component of
the mixture, particularly when the data are obtained in methanol. These
experiments also demonstrate the feasibility of detailed NMR structural
investigations in model systems for glyceride digestion.
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