Journal of Lipid Research, Vol 33, 549-557, Copyright © 1992 by Lipid Research, Inc.

ARTICLES

Structure of the hamster low density lipoprotein receptor gene

RW Bishop
Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas 75235.

The metabolism of low density lipoprotein (LDL) in the hamster is substantially similar to that of the human. To extend the usefulness of the hamster as an experimental model, the hamster LDL receptor gene was isolated and characterized. The gene is composed of 18 exons and 17 introns which span 26 kilobases. The introns occur at precisely the same positions as those previously determined for the human LDL receptor gene. The 18 exons of the hamster gene predict an LDL receptor protein of 854 amino acids that is similar in organization and sequence to those predicted from the cDNAs of rat, rabbit, cow, and human. Within the 5'-flanking region of the hamster LDL receptor gene are three highly conserved imperfect direct repeat sequences of 16 nucleotides each that in the human gene have been demonstrated to regulate transcription. In addition, a similar arrangement of direct repeat sequences was also isolated from the 5'-flanking region of the rat LDL receptor gene using the polymerase chain reaction. These results indicate a strong sequence and structural conservation of the LDL receptor among several species and further support the hamster as an experimental model for the study of human LDL-cholesterol metabolism.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				D. Lopez and M. P. McLean Sterol Regulatory Element-Binding Protein-1a Binds to cis Elements in the Promoter of the Rat High Density Lipoprotein Receptor SR-BI Gene Endocrinology, December 1, 1999; 140(12): 5669 - 5681. [Abstract] [Full Text]

				T. Ravid, R. Avner, S. Polak-Charcon, J. R. Faust, and J. Roitelman Impaired Regulation of 3-Hydroxy-3-methylglutaryl-Coenzyme A Reductase Degradation in Lovastatin-resistant Cells J. Biol. Chem., October 8, 1999; 274(41): 29341 - 29351. [Abstract] [Full Text] [PDF]

				K. A. A. Grunwald, K. Schueler, P. J. Uelmen, B. A. Lipton, M. Kaiser, K. Buhman, and A. D. Attie Identification of a novel Arg'Cys mutation in the LDL receptor that contributes to spontaneous hypercholesterolemia in pigs J. Lipid Res., March 1, 1999; 40(3): 475 - 485. [Abstract] [Full Text]

				H. A. LaVoie, J. C. Garmey, R. N. Day, and J. D. Veldhuis Concerted Regulation of Low Density Lipoprotein Receptor Gene Expression by Follicle-Stimulating Hormone and Insulin-Like Growth Factor I in Porcine Granulosa Cells: Promoter Activation, Messenger Ribonucleic Acid Stability, and Sterol Feedback Endocrinology, January 1, 1999; 140(1): 178 - 186. [Abstract] [Full Text]

				J. V. Swinnen, P. Alen, W. Heyns, and G. Verhoeven Identification of Diazepam-binding Inhibitor/Acyl-CoA-binding Protein as a Sterol Regulatory Element-binding Protein-responsive Gene J. Biol. Chem., August 7, 1998; 273(32): 19938 - 19944. [Abstract] [Full Text] [PDF]

				I. Shimomura, Y. Bashmakov, H. Shimano, J. D. Horton, J. L. Goldstein, and M. S. Brown Cholesterol feeding reduces nuclear forms of sterol regulatory element binding proteins in hamster liver PNAS, November 11, 1997; 94(23): 12354 - 12359. [Abstract] [Full Text] [PDF]

				H. Jeon and G. G. Shipley Localization of the N-terminal Domain of the Low Density Lipoprotein Receptor J. Biol. Chem., September 22, 2000; 275(39): 30465 - 30470. [Abstract] [Full Text] [PDF]

				H. Jeon and G. G. Shipley Vesicle-reconstituted Low Density Lipoprotein Receptor. VISUALIZATION BY CRYOELECTRON MICROSCOPY J. Biol. Chem., September 22, 2000; 275(39): 30458 - 30464. [Abstract] [Full Text] [PDF]