Journal of Lipid Research, Vol 33, 1084-1088, Copyright © 1992 by Lipid Research, Inc.

ARTICLES

A simple and rapid method for the preparation of apolipoproteins for electrophoresis

MA Mindham and PA Mayes
Department of Veterinary Basic Sciences, Royal Veterinary College (University of London), United Kingdom.

A new method is described for the rapid preparation of apolipoproteins for polyacrylamide gel electrophoresis. It is suitable for all serum lipoproteins including chylomicrons. The procedure involves extraction with diethyl ether in the presence of trichloroacetic acid and sodium deoxycholate. The method gives an improved protein recovery, in particular with chylomicrons. In addition, samples do not require dialysis to remove salts (e.g., KBr) prior to processing; as a consequence, the procedure requires only 1 h. Due to this rapidity and the high yields, the procedure is superior to present methods utilizing ethanol-diethyl ether extraction.
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