Journal of Lipid Research, Vol 33, 1383-1391, Copyright © 1992 by Lipid Research, Inc.
Enterohepatic circulation in hamsters with an extracorporeal bile duct
M Fuchs, J Scheibner, E Hormann, G Tauber and EF Stange
Department of Internal Medicine II, University of Ulm, Germany.
The present study describes a novel technique for investigations of the
enterohepatic circulation in the hamster with an extracorporeal bile duct
that allows long-term bile collection in the free-moving animal. The
animals recovered for 7 days after the operation before the external loop
was cut and bile was collected over a period of 78 h. Under these optimal
conditions, initial bile flow (651 +/- 89 microliters per 100 g.h-1) and
the secretion rates of biliary lipids were several-fold higher than
reported in an earlier study using the acute fistula hamster. Biliary
cholesterol secretion amounted to 369 +/- 32 nmol per 100 g.h-1,
phospholipid secretion was 2.6 +/- 0.3 mumol per 100 g.h-1, and total bile
acid secretion was 31.9 +/- 2.2 mumol per 100 g.h-1. A clearcut diurnal
rhythm was demonstrated for bile flow and all biliary constituents. After 9
h the depletion of the bile acid pool was complete and cholic acid
synthesis derepressed 1.4-fold from a basal rate of 818 nmol per 100 g.h-1,
whereas the derepression of chenodeoxycholic acid synthesis was even less
pronounced. Biliary cholesterol output increased 2.2-fold, but the
phospholipid secretion was constant during the full experiment. It may be
concluded that the technique of an extracorporeal bile duct in the
free-moving animal allows studies of bile secretion under optimal
conditions. Most likely the bile secretion rates given above approach the
physiological rates in the hamster.
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