Journal of Lipid Research, Vol 34, 1699-1707, Copyright © 1993 by Lipid Research, Inc.

ARTICLES

Localization of a liver-specific enhancer in the apolipoprotein E/C-I/C- II gene locus

NS Shachter, Y Zhu, A Walsh, JL Breslow and JD Smith
Laboratory of Biochemical Genetics and Metabolism, Rockefeller University, New York, NY 10021-6399.

The sequences necessary for liver-specific expression of the apolipoprotein (apo) E gene have been shown to reside 3' to the gene, within the apoE/C-I/C-II gene cluster, but have not been precisely characterized. Utilizing a transient transfection reporter gene assay based on the apoC-II promoter, we have localized a liver-specific enhancer to its approximate limit dimension of 154 base pairs. This enhancer directed liver-specific expression of an apoE gene construction in transgenic mice. A DNaseI protection assay revealed two footprints over an inverted repeat of a known transcriptionally active motif, TGACCT. DNaseI-sensitive sites were present in three of six repeats of a motif (consensus GCAAACA) which has been postulated to represent the recognition sequence of a hepatic transcriptional activity, HNF-5. This region of DNA may function as a liver-specific enhancer for the entire apoE/C-I/C-II gene cluster.
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