J. Lipid Res.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ostlund, R. E.
Right arrow Articles by Matthews, D. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ostlund, R. E., Jr
Right arrow Articles by Matthews, D. E.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Journal of Lipid Research, Vol 34, 1825-1831, Copyright © 1993 by Lipid Research, Inc.

ARTICLES

[13C]cholesterol as a tracer for studies of cholesterol metabolism in humans

RE Ostlund Jr and DE Matthews
Department of Internal Medicine, Washington University School of Medicine, St. Louis, MO 63110.

The investigation of cholesterol metabolism in humans would be facilitated by the availability of a tracer that is not radioactive. However, to be useful such a tracer must be detectable in the large pool of body cholesterol over an extended time. To meet these requirements we used synthetic [23,24,25,26,27-13C5]cholesterol with detection by isotope ratio mass spectrometry (IRMS), a technique in which the ratio of 13CO2/12CO2 can be determined with high precision in the combustion products of a biological sample. [13C]cholesterol (65 mg) and [14C]cholesterol (15 microCi) were solubilized in Intralipid and injected simultaneously (IV) into three normal subjects, and plasma samples were obtained over the ensuing 10 weeks. Cholesterol was isolated from plasma and either counted for radioactivity or combusted to CO2 and analyzed by IRMS. [14C]cholesterol and [13C]cholesterol tracer concentrations in plasma were very similar. [13C]cholesterol kinetic parameters calculated using a standard two-compartment cholesterol turnover model and a new three-compartment minimal model were 103 +/- 10.5 SD % of those computed from corresponding [14C]cholesterol data. These results show that [13C5]cholesterol can be used as a practical tracer for cholesterol metabolic studies lasting many weeks.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
F. Pont, L. Duvillard, B. Verges, and P. Gambert
Development of Compartmental Models in Stable-Isotope Experiments : Application to Lipid Metabolism
Arterioscler. Thromb. Vasc. Biol., June 1, 1998; 18(6): 853 - 860.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
R. E. Ostlund Jr., J. B. McGill, C.-M. Zeng, D. F. Covey, J. Stearns, W. F. Stenson, and C. A. Spilburg
Gastrointestinal absorption and plasma kinetics of soy Delta 5-phytosterols and phytostanols in humans
Am J Physiol Endocrinol Metab, April 1, 2002; 282(4): E911 - E916.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 1993 by the American Society for Biochemistry and Molecular Biology.