Journal of Lipid Research, Vol 34, 1895-1900, Copyright © 1993 by Lipid Research, Inc.

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Derivatization of bile acids with taurine for analysis by fast atom bombardment mass spectrometry with collision-induced fragmentation

J Zhang, WJ Griffiths, T Bergman and J Sjovall
Department of Physiological Chemistry, Karolinska Institutet, Stockholm, Sweden.

When analyzed by fast atom bombardment mass spectrometry, taurine- conjugated bile acids give intense [M-H]-pseudomolecular ions that can be subjected to collision-induced fragmentation to give structural information. A method has been developed that permits rapid coupling of taurine to unconjugated, glycine-conjugated, sulfated, and glucuronidated bile acids. The reaction is performed for 2 h at room temperature in aqueous pyridine hydrochloride buffer, with or without dioxane, using 0.1 M 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide as the coupling agent and 0.2 M taurine. The yields are higher than 95%. In contrast to published coupling reactions, the method permits conjugation of bile acids with the labile 7 alpha-hydroxy-3-oxo-4-ene structure.
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