| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Journal of Lipid Research, Vol 34, 1931-1942, Copyright © 1993 by Lipid Research, Inc.
ARTICLES |
M VanRollins, TL Kaduce, HR Knapp and AA Spector
Department of Biochemistry, University of Iowa, Iowa City 52242.
Epoxyeicosatrienoic acid (EET) metabolism was studied in endothelial cells to determine whether this tissue may influence their vasoactive properties. Porcine aortic endothelial cells rapidly took up all four EET regioisomers. The uptake of [1-14C]14,15-EET reached a maximum in 15-30 min, and saturation was not observed with concentrations up to 5 microM. More than 70% of the incorporated 14,15-EET was contained in choline and inositol glycerophospholipids, most of it in the form of an EET ester. A metabolite, 14,15-dihydroxyeicosatrienoic acid (14,15- DHET), accumulated in the medium during incubation, and products with similar chromatographic properties also were formed from 5,6-, 8,9-, and 11,12-EET. Much of the 14,15-EET taken up was only temporarily retained by the cells, and in 2 h half was released into the medium as 14,15-DHET. Bovine aortic and human umbilical vein endothelial cells also took up 14,15-EET, incorporated it into choline glycerophospholipids, and converted it to 14,15-DHET. These findings suggest that the endothelium may limit the vascular actions of EETs through rapid uptake, hydration, and release of DHETs into the circulation. Some vasoactive effects of EETs may result from their temporary accumulation in endothelial phospholipids involved in stimulus-response coupling.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  R. M. Nusing, H. Schweer, I. Fleming, D. C. Zeldin, and M. Wegmann Epoxyeicosatrienoic acids affect electrolyte transport in renal tubular epithelial cells: dependence on cyclooxygenase and cell polarity Am J Physiol Renal Physiol, July 1, 2007; 293(1): F288 - F298. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. A. Spector and A. W. Norris Action of epoxyeicosatrienoic acids on cellular function Am J Physiol Cell Physiol, March 1, 2007; 292(3): C996 - C1012. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Liu, Y. Zhang, K. Schmelzer, T.-S. Lee, X. Fang, Y. Zhu, A. A. Spector, S. Gill, C. Morisseau, B. D. Hammock, et al. The antiinflammatory effect of laminar flow: The role of PPAR{gamma}, epoxyeicosatrienoic acids, and soluble epoxide hydrolase PNAS, November 15, 2005; 102(46): 16747 - 16752. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. You, E. M. Golding, and R. M. Bryan Jr. Arachidonic acid metabolites, hydrogen peroxide, and EDHF in cerebral arteries Am J Physiol Heart Circ Physiol, September 1, 2005; 289(3): H1077 - H1083. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Jung, R. P. Brandes, I.-H. Kim, F. Schweda, R. Schmidt, B. D. Hammock, R. Busse, and I. Fleming Soluble Epoxide Hydrolase Is a Main Effector of Angiotensin II-Induced Hypertension Hypertension, April 1, 2005; 45(4): 759 - 765. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Yang, K. M. Gauthier, L. M. Reddy, B. Sangras, K. K. Sharma, K. Nithipatikom, J. R. Falck, and W. B. Campbell Stable 5,6-Epoxyeicosatrienoic Acid Analog Relaxes Coronary Arteries Through Potassium Channel Activation Hypertension, April 1, 2005; 45(4): 681 - 686. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Fang, N. L. Weintraub, R. B. McCaw, S. Hu, S. D. Harmon, J. B. Rice, B. D. Hammock, and A. A. Spector Effect of soluble epoxide hydrolase inhibition on epoxyeicosatrienoic acid metabolism in human blood vessels Am J Physiol Heart Circ Physiol, December 1, 2004; 287(6): H2412 - H2420. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Nithipatikom, E. R. Gross, M. P. Endsley, J. M. Moore, M. A. Isbell, J. R. Falck, W. B. Campbell, and G. J. Gross Inhibition of Cytochrome P450{omega}-Hydroxylase: A Novel Endogenous Cardioprotective Pathway Circ. Res., October 15, 2004; 95(8): e65 - e71. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Spiecker, H. Darius, T. Hankeln, M. Soufi, A. M. Sattler, J. R. Schaefer, K. Node, J. Borgel, A. Mugge, K. Lindpaintner, et al. Risk of Coronary Artery Disease Associated With Polymorphism of the Cytochrome P450 Epoxygenase CYP2J2 Circulation, October 12, 2004; 110(15): 2132 - 2136. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Yu, B. B. Davis, C. Morisseau, B. D. Hammock, J. L. Olson, D. L. Kroetz, and R. H. Weiss Vascular localization of soluble epoxide hydrolase in the human kidney Am J Physiol Renal Physiol, April 1, 2004; 286(4): F720 - F726. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. L. Kaduce, X. Fang, S. D. Harmon, C. L. Oltman, K. C. Dellsperger, L. M. Teesch, V. R. Gopal, J. R. Falck, W. B. Campbell, N. L. Weintraub, et al. 20-Hydroxyeicosatetraenoic Acid (20-HETE) Metabolism in Coronary Endothelial Cells J. Biol. Chem., January 23, 2004; 279(4): 2648 - 2656. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Falck, U. M. Krishna, Y. K. Reddy, P. S. Kumar, K. M. Reddy, S. B. Hittner, C. Deeter, K. K. Sharma, K. M. Gauthier, and W. B. Campbell Comparison of vasodilatory properties of 14,15-EET analogs: structural requirements for dilation Am J Physiol Heart Circ Physiol, January 1, 2003; 284(1): H337 - H349. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Fang, N. L. Weintraub, C. L. Oltman, L. L. Stoll, T. L. Kaduce, S. Harmon, K. C. Dellsperger, C. Morisseau, B. D. Hammock, and A. A. Spector Human coronary endothelial cells convert 14,15-EET to a biologically active chain-shortened epoxide Am J Physiol Heart Circ Physiol, December 1, 2002; 283(6): H2306 - H2314. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. W. Newman, T. Watanabe, and B. D. Hammock The simultaneous quantification of cytochrome P450 dependent linoleate and arachidonate metabolites in urine by HPLC-MS/MS J. Lipid Res., September 1, 2002; 43(9): 1563 - 1578. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Subbanagounder, J. W. Wong, H. Lee, K. F. Faull, E. Miller, J. L. Witztum, and J. A. Berliner Epoxyisoprostane and Epoxycyclopentenone Phospholipids Regulate Monocyte Chemotactic Protein-1 and Interleukin-8 Synthesis. FORMATION OF THESE OXIDIZED PHOSPHOLIPIDS IN RESPONSE TO INTERLEUKIN-1beta J. Biol. Chem., February 22, 2002; 277(9): 7271 - 7281. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J. Roman P-450 Metabolites of Arachidonic Acid in the Control of Cardiovascular Function Physiol Rev, January 1, 2002; 82(1): 131 - 185. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Fleming Cytochrome P450 and Vascular Homeostasis Circ. Res., October 26, 2001; 89(9): 753 - 762. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Yang, L. Graham, S. Dikalov, R. P. Mason, J. R. Falck, J. K. Liao, and D. C. Zeldin Overexpression of Cytochrome P450 CYP2J2 Protects against Hypoxia-Reoxygenation Injury in Cultured Bovine Aortic Endothelial Cells Mol. Pharmacol., August 1, 2001; 60(2): 310 - 320. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zhang, C. L. Oltman, T. Lu, H.-C. Lee, K. C. Dellsperger, and M. VanRollins EET homologs potently dilate coronary microvessels and activate BKCa channels Am J Physiol Heart Circ Physiol, June 1, 2001; 280(6): H2430 - H2440. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Rastaldo, N. Paolocci, A. Chiribiri, C. Penna, D. Gattullo, and P. Pagliaro Cytochrome P-450 metabolite of arachidonic acid mediates bradykinin-induced negative inotropic effect Am J Physiol Heart Circ Physiol, June 1, 2001; 280(6): H2823 - H2832. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Imig Eicosanoid regulation of the renal vasculature Am J Physiol Renal Physiol, December 1, 2000; 279(6): F965 - F981. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Navab, S. Y. Hama, C. J. Cooke, G. M. Anantharamaiah, M. Chaddha, L. Jin, G. Subbanagounder, K. F. Faull, S. T. Reddy, N. E. Miller, et al. Normal high density lipoprotein inhibits three steps in the formation of mildly oxidized low density lipoprotein: step 1 J. Lipid Res., September 1, 2000; 41(9): 1481 - 1494. [Abstract] [Full Text]
|
|
|
|
|
|
X. Fang, T. L. Kaduce, M. VanRollins, N. L. Weintraub, and A. A. Spector Conversion of epoxyeicosatrienoic acids (EETs) to chain-shortened epoxy fatty acids by human skin fibroblasts J. Lipid Res., January 1, 2000; 41(1): 66 - 74. [Abstract] [Full Text]
|
|
|
|
|
|
N. L. Weintraub, X. Fang, T. L. Kaduce, M. VanRollins, P. Chatterjee, and A. A. Spector Epoxide hydrolases regulate epoxyeicosatrienoic acid incorporation into coronary endothelial phospholipids Am J Physiol Heart Circ Physiol, November 1, 1999; 277(5): H2098 - H2108. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Node, Y. Huo, X. Ruan, B. Yang, M. Spiecker, K. Ley, D. C. Zeldin, and J. K. Liao Anti-inflammatory Properties of Cytochrome P450 Epoxygenase-Derived Eicosanoids Science, August 20, 1999; 285(5431): 1276 - 1279. [Abstract] [Full Text]
|
|
|
|
|
|
C. L. Oltman, N. L. Weintraub, M. VanRollins, and K. C. Dellsperger Epoxyeicosatrienoic Acids and Dihydroxyeicosatrienoic Acids Are Potent Vasodilators in the Canine Coronary Microcirculation Circ. Res., November 2, 1998; 83(9): 932 - 939. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Fulton, J. R. Falck, J. C. McGiff, M. A. Carroll, and J. Quilley A method for the determination of 5,6-EET using the lactone as an intermediate in the formation of the diol J. Lipid Res., August 1, 1998; 39(8): 1713 - 1721. [Abstract] [Full Text]
|
|
|
|
|
|
X. Fang, N. L. Weintraub, C. D. Rios, D. A. Chappell, R. M. Zwacka, J. F. Engelhardt, L. W. Oberley, T. Yan, D. D. Heistad, and A. A. Spector Overexpression of Human Superoxide Dismutase Inhibits Oxidation of Low-Density Lipoprotein by Endothelial Cells Circ. Res., June 29, 1998; 82(12): 1289 - 1297. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. E. Williard, T. L. Kaduce, S. D. Harmon, and A. A. Spector Conversion of eicosapentaenoic acid to chain-shortened omega–3 fatty acid metabolites by peroxisomal oxidation J. Lipid Res., May 1, 1998; 39(5): 978 - 986. [Abstract] [Full Text]
|
|
|
|
|
|
N. L. Weintraub, X. Fang, T. L. Kaduce, M. VanRollins, P. Chatterjee, and A. A. Spector Potentiation of Endothelium-Dependent Relaxation by Epoxyeicosatrienoic Acids Circ. Res., August 19, 1997; 81(2): 258 - 267. [Abstract] [Full Text]
|
|
|
|
|
|
X. Fang, T. L. Kaduce, N. L. Weintraub, M. VanRollins, and A. A. Spector Functional Implications of a Newly Characterized Pathway of 11,12-Epoxyeicosatrienoic Acid Metabolism in Arterial Smooth Muscle Circ. Res., October 1, 1996; 79(4): 784 - 793. [Abstract] [Full Text]
|
|
|
|
|
|
M. VanRollins, T. L. Kaduce, X. Fang, H. R. Knapp, and A. A. Spector Arachidonic Acid Diols Produced by Cytochrome P-450 Monooxygenases Are Incorporated into Phospholipids of Vascular Endothelial Cells J. Biol. Chem., June 14, 1996; 271(24): 14001 - 14009. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Fang, T. L. Kaduce, N. L. Weintraub, S. Harmon, L. M. Teesch, C. Morisseau, D. A. Thompson, B. D. Hammock, and A. A. Spector Pathways of Epoxyeicosatrienoic Acid Metabolism in Endothelial Cells. IMPLICATIONS FOR THE VASCULAR EFFECTS OF SOLUBLE EPOXIDE HYDROLASE INHIBITION J. Biol. Chem., April 27, 2001; 276(18): 14867 - 14874. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Salvail, M. Cloutier, and E. Rousseau Functional reconstitution of an eicosanoid-modulated Cl- channel from bovine tracheal smooth muscle Am J Physiol Cell Physiol, March 1, 2002; 282(3): C567 - C577. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. B. Campbell, C. Deeter, K. M. Gauthier, R. H. Ingraham, J. R. Falck, and P.-L. Li 14,15-Dihydroxyeicosatrienoic acid relaxes bovine coronary arteries by activation of KCa channels Am J Physiol Heart Circ Physiol, May 1, 2002; 282(5): H1656 - H1664. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |
