Journal of Lipid Research, Vol 34, 359-364, Copyright © 1993 by Lipid Research, Inc.
Clofibrate elevates enzyme activities of the tricarboxylic acid cycle in rat liver
C Prager, HJ Schon, M Nikfardjam, D Schmid, M Untersalmberger, K Kremser and R Kramar
Department of Medical Chemistry, University of Vienna, Austria.
Activities of the tricarboxylic acid cycle enzymes were measured in
subcellular fractions of liver from rats that had been fed clofibrate for 3
weeks. Large changes in these activities per gram tissue were found in the
large particle fraction, which also showed an increase in total protein
concentration of 76% under clofibrate treatment. The three regulatory
enzymes of the cycle, namely citrate synthase, NAD(+)- linked isocitrate
dehydrogenase, and 2-oxoglutarate dehydrogenase were significantly enhanced
by 24% (P < 0.02), 54% (P < 0.02), and 153% (P < 0.005),
respectively. Fumarase and malate dehydrogenase rose by 71% (P < 0.005)
and 95% (P < 0.02), whereas succinate dehydrogenase remained unchanged.
Enhancement of the citrate synthase, NAD-isocitrate dehydrogenase, and
2-oxoglutarate dehydrogenase may play a role in decreasing intracellular
availability of acetyl-CoA for lipid metabolism.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
