HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Tertov, V. V. Articles by Guevara, J. G. Search for Related Content PubMed PubMed Citation Articles by Tertov, V. V. Articles by Guevara, J. G., Jr Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 34, 365-375, Copyright © 1993 by Lipid Research, Inc.

ARTICLES

Carbohydrate composition of protein and lipid components in sialic acid- rich and -poor low density lipoproteins from subjects with and without coronary artery disease

VV Tertov, AN Orekhov, IA Sobenin, JD Morrisett, AM Gotto Jr and JG Guevara Jr
Institute of Experimental Cardiology, Russian Cardiology Research Center, Moscow.

Low density lipoprotein (LDL) from patients with coronary heart disease (CHD) caused 78-286% increase in accumulation of cholesterol in human aortic subendothelial cells compared to 2-17% caused by LDL from normal subjects. Ricin-Sepharose affinity chromatography was used to separate LDL into two subfractions, one sialic acid-rich (SAR) and the other sialic acid-poor (SAP). SAP-LDL from CHD patients caused 156-307% increase in accumulation of cellular cholesterol, whereas SAR-LDL from these patients caused only 14-21% increase. SAP-LDL from normal healthy subjects caused 50-86% increased accumulation, whereas their SAR-LDL induced only 2-12% increase. Carbohydrate analysis of SAP-LDL protein isolated from four CHD patients revealed mean values of 59, 25, 61, and 11 nmoles of N-acetyl glucosamine, galactose, mannose, and sialic acid per mg protein, respectively. Mean values for SAR-LDL protein from these patients were 59, 31, 77, and 24 nmol/mg protein, respectively. Analysis of SAP-LDL protein from four normal healthy subjects indicated respective mean values of 58, 29, 72, and 22 nmol/mg, whereas SAR-LDL protein from normals contained 59, 29, 72, and 29 nmol/mg. The carbohydrate content of LDL lipids represents about 25% of the total carbohydrate present in the lipoprotein. The mean values for SAP-LDL lipids from four CHD patients were about 2, 2, 18, 18, and 2 nmol/mg protein for N-acetyl galactosamine, N-acetyl glucosamine, galactose, glucose, and sialic acid, respectively. The mean values for SAR-LDL lipids from these patients were 3, 4, 34, 41, and 5 nmol/mg, respectively. Analysis of SAP-LDL lipids from four normal healthy subjects indicated respective mean values of 4, 6, 30, 31, and 3 nmol/mg, whereas SAR-LDL lipids from these subjects contained 6, 9, 41, 46, and 7 nmol/mg. These results suggest that the different biological properties of SAR-LDL and SAP-LDL are related to their different carbohydrate compositions.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				B. Garner, D. J. Harvey, L. Royle, M. Frischmann, F. Nigon, M. J. Chapman, and P. M. Rudd Characterization of human apolipoprotein B100 oligosaccharides in LDL subfractions derived from normal and hyperlipidemic plasma: deficiency of {alpha}-N-acetylneuraminyllactosyl-ceramide in light and small dense LDL particles Glycobiology, October 1, 2001; 11(10): 791 - 802. [Abstract] [Full Text] [PDF]

				I. A. Sobenin, V. V. Tertov, and A. N. Orekhov Optimization of the assay for sialic acid determination in low density lipoprotein J. Lipid Res., November 1, 1998; 39(11): 2293 - 2299. [Abstract] [Full Text]

				B. Chappey, B. Beyssen, E. Foos, F. Ledru, J. L. Guermonprez, J. C. Gaux, and I. Myara Sialic Acid Content of LDL in Coronary Artery Disease: No Evidence of Desialylation in Subjects With Coronary Stenosis and Increased Levels in Subjects With Extensive Atherosclerosis and Acute Myocardial Infarction : Relation Between Desialylation and In Vitro Peroxidation Arterioscler. Thromb. Vasc. Biol., June 1, 1998; 18(6): 876 - 883. [Abstract] [Full Text] [PDF]

				B. Chappey, I. Myara, P. Giral, G. Kerharo, M.C. Plainfosse, J. Levenson, A. Simon, and N. Moatti Evaluation of the Sialic Acid Content of LDL as a Marker of Coronary Calcification and Extracoronary Atherosclerosis in Asymptomatic Hypercholesterolemic Subjects Arterioscler. Thromb. Vasc. Biol., March 1, 1995; 15(3): 334 - 339. [Abstract] [Full Text]

				E. Malle, A. Ibovnik, H. J. Leis, G. M. Kostner, P. F. J. Verhallen, and W. Sattler Lysine Modification of LDL or Lipoprotein(a) by 4-Hydroxynonenal or Malondialdehyde Decreases Platelet Serotonin Secretion Without Affecting Platelet Aggregability and Eicosanoid Formation Arterioscler. Thromb. Vasc. Biol., March 1, 1995; 15(3): 377 - 384. [Abstract] [Full Text]