HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Takeuchi, N. Articles by Chino, H. Search for Related Content PubMed PubMed Citation Articles by Takeuchi, N. Articles by Chino, H. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 34, 543-551, Copyright © 1993 by Lipid Research, Inc.

ARTICLES

Lipid transfer particle in the hemolymph of the American cockroach: evidence for its capacity to transfer hydrocarbons between lipophorin particles

N Takeuchi and H Chino
Biochemical Laboratory, Hokkaido University, Sapporo, Japan.

A lipid transfer particle (LTP) was isolated and purified from the hemolymph of the adult male American cockroach, Periplaneta americana, essentially according to the method previously developed for the purification of LTP from locust hemolymph. Fast protein liquid chromatography (FPLC) on a Mono Q column was used as the additional step to obtain pure LTP. The electron micrograph of purified cockroach LTP exhibited an unusual and asymmetric shape essentially similar to that reported for Manduca sexta LTP (Ryan et al. 1990. J. Lipid Res. 31:871-879). The cockroach LTP was also found to be basically similar to that of M. sexta and locust in terms of subunit structure and lipid composition, although there were significant differences particularly in the contents of hydrocarbons and diacylglycerol. A simple method for assaying LTP activity was developed, based on the finding that cockroach LTP can catalyze the transfer of labeled hydrocarbons (or diacylglycerol) from labeled high density lipophorin (HDLp) bound with a transfer membrane (Immoblion) to unlabeled HDLp dissolved in saline. This finding reveals that cockroach LTP has the capacity to transfer and/or exchange lipids between lipophorin particles with the same density. It was also demonstrated that cockroach LTP has the capacity to catalyze the transfer and/or exchange of hydrocarbons, in addition to diacylglycerol, between cockroach HDLp and locust low density lipophorin (LDLp).
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				L. E. Canavoso, H. K. Yun, Z. E. Jouni, and M. A. Wells Lipid transfer particle mediates the delivery of diacylglycerol from lipophorin to fat body in larval Manduca sexta J. Lipid Res., March 1, 2004; 45(3): 456 - 465. [Abstract] [Full Text] [PDF]

				Y. Fan, J. Chase, V. L. Sevala, and C. Schal Lipophorin-facilitated hydrocarbon uptake by oocytes in the German cockroach Blattella germanica (L.) J. Exp. Biol., March 15, 2002; 205(6): 781 - 790. [Abstract] [Full Text] [PDF]