J. Lipid Res.
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Journal of Lipid Research, Vol 34, 625-631, Copyright © 1993 by Lipid Research, Inc.

ARTICLES

Metabolism of 12(S)-hydroxy-5,8,10,14-eicosatetraenoic acid by kidney and liver peroxisomes

J Wigren, H Herbertsson, O Tollbom and S Hammarstrom
Department of Cell Biology, Linkoping University, Sweden.

12(S)-Hydroperoxy-5,8,10,14-eicosatetraenoic acid (12(S)-HPETE) is one of the main products formed from arachidonic acid by animal lipoxygenases. It is usually reduced to the corresponding hydroxy acid, 12(S)-hydroxy-5,8,10,14-eicosatetraenoic acid (12(S)-HETE) or it can serve as an intermediate in the biosynthesis of hepoxilins. The functions of 12(S)-HETE are incompletely understood but it has been proposed that it plays a role in the metastatic process of certain cancer cells, including Lewis lung carcinoma cells. 12(S)-HETE is metabolically degraded by beta- or omega-oxidation. Indirect evidence has suggested involvement of peroxisomes in the beta-oxidative degradation. The purpose of the present investigation was to study 12(S)-HETE metabolism by peroxisomes isolated from rat liver or kidney by sucrose density gradient centrifugation. The purity of subcellular fractions was determined by marker enzyme analyses and electron microscopy. 12(S)-HETE was quantitatively converted by liver or kidney peroxisomes into a single, diethyl ether-extractable metabolite. This was identified by gas-liquid chromatography-mass spectrometry as 8- hydroxyhexadecatrienoic acid. Lewis lung carcinoma cells also metabolized 12(S)-HETE to 8-hydroxyhexadecatrienoic acid.
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D. L. Luthria, S. P. Baykousheva, and H. Sprecher
Double Bond Removal from Odd-numbered Carbons during Peroxisomal [IMAGE]-Oxidation of Arachidonic Acid Requires both 2,4-Dienoyl-CoA Reductase and [IMAGE][IMAGE],[IMAGE][IMAGE]-Dienoyl-CoA Isomerase
J. Biol. Chem., June 9, 1995; 270(23): 13771 - 13776.
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