Journal of Lipid Research, Vol 34, 1047-1053, Copyright © 1993 by Lipid Research, Inc.

ARTICLES

Assessment of percent cholesterol absorption in humans with stable isotopes

MS Bosner, RE Ostlund Jr, O Osofisan, J Grosklos, C Fritschle and LG Lange
Division of Cardiology, Jewish Hospital of St. Louis, Washington University School of Medicine.

Dietary cholesterol restriction is a general recommendation for the medical community and emphasizes the importance of intestinal cholesterol absorption and metabolism in humans. However, several methods that may accurately quantify cholesterol absorption utilize radioactive isotopes that are undesirable for younger individuals, women, children, and normal subjects. To eliminate this hazard, we have developed a procedure for measurement of percent cholesterol absorption, based on that of Zilversmit (1972. Proc. Soc. Exp. Med. Biol. 140: 862-865), using stable nonradioactive isotopic tracers of cholesterol. [26,26,26,27,27,27-2H]cholesterol (30 mg) was administered orally and [23,24,25,26,27-13C]cholesterol (15 mg) was administered intravenously on day 0 and percent cholesterol absorption was calculated as the plasma ratio of oral/intravenous isotopic tracer on day 3 as determined by gas chromatography-mass spectrometry with selected ion monitoring. Tracer cholesterol given orally peaked in plasma on day 2 and then slowly declined in parallel with the intravenous tracer. Cholesterol absorption in 16 healthy subjects (on no medication and not ingesting alcohol) consuming a Step One Diet was 53.5% +/- 8.5 SD%. Five subjects underwent repeat testing after 4-6 weeks with excellent replication (SD of difference between tests = 2.8%). No differences in the metabolism of [13C5]cholesterol, [2H6]cholesterol, and [14C]cholesterol were observed. The use of stable isotopes for the study of percent cholesterol absorption is precise and safe, allowing repeated measurements in normal individuals and thus facilitating clinical investigation of this key component of human cholesterol metabolism.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				S. Santosa, I. Demonty, A. H. Lichtenstein, J. M. Ordovas, and P. J. H. Jones Single nucleotide polymorphisms in ABCG5 and ABCG8 are associated with changes in cholesterol metabolism during weight loss J. Lipid Res., December 1, 2007; 48(12): 2607 - 2613. [Abstract] [Full Text] [PDF]

				L A Woollett, Y Wang, D D Buckley, L Yao, S Chin, N Granholm, P J H Jones, K D R Setchell, P Tso, and J E Heubi Micellar solubilisation of cholesterol is essential for absorption in humans Gut, February 1, 2006; 55(2): 197 - 204. [Abstract] [Full Text] [PDF]

				Y. H. Lin, R. J. Pawlosky, and N. Salem Jr. Simultaneous quantitative determination of deuterium- and carbon-13-labeled essential fatty acids in rat plasma J. Lipid Res., September 1, 2005; 46(9): 1974 - 1982. [Abstract] [Full Text] [PDF]

				L. A. Woollett, D. D. Buckley, L. Yao, P. J. H. Jones, N. A. Granholm, E. A. Tolley, and J. E. Heubi Effect of ursodeoxycholic acid on cholesterol absorption and metabolism in humans J. Lipid Res., May 1, 2003; 44(5): 935 - 942. [Abstract] [Full Text] [PDF]

				C. A Vanstone, M. Raeini-Sarjaz, W. E Parsons, and P. J. Jones Unesterified plant sterols and stanols lower LDL-cholesterol concentrations equivalently in hypercholesterolemic persons Am. J. Clinical Nutrition, December 1, 2002; 76(6): 1272 - 1278. [Abstract] [Full Text] [PDF]

				G. Beaumier-Gallon, C. Dubois, M. Senft, M.-F. Vergnes, A.-M. Pauli, H. Portugal, and D. Lairon Dietary cholesterol is secreted in intestinally derived chylomicrons during several subsequent postprandial phases in healthy humans Am. J. Clinical Nutrition, May 1, 2001; 73(5): 870 - 877. [Abstract] [Full Text] [PDF]

				P. J. Jones, M. Raeini-Sarjaz, F. Y. Ntanios, C. A. Vanstone, J. Y. Feng, and W. E. Parsons Modulation of plasma lipid levels and cholesterol kinetics by phytosterol versus phytostanol esters J. Lipid Res., May 1, 2000; 41(5): 697 - 705. [Abstract] [Full Text]

				R. E Ostlund Jr, C. A Spilburg, and W. F Stenson Sitostanol administered in lecithin micelles potently reduces cholesterol absorption in humans Am. J. Clinical Nutrition, November 1, 1999; 70(5): 826 - 831. [Abstract] [Full Text] [PDF]

				R. E. Ostlund , Jr., M. S. Bosner, and W. F. Stenson Cholesterol absorption efficiency declines at moderate dietary doses in normal human subjects J. Lipid Res., August 1, 1999; 40(8): 1453 - 1458. [Abstract] [Full Text]

				M. S. Bosner, L. G. Lange, W. F. Stenson, and R. E. Ostlund , Jr. Percent cholesterol absorption in normal women and men quantified with dual stable isotopic tracers and negative ion mass spectrometry J. Lipid Res., February 1, 1999; 40(2): 302 - 308. [Abstract] [Full Text]

				L. H. Krut, J. W. Yang, G. Schonfeld, and R. E. Ostlund Jr The Effect of Oxidizing Cholesterol on Gastrointestinal Absorption, Plasma Clearance, Tissue Distribution, and Processing by Endothelial Cells Arterioscler. Thromb. Vasc. Biol., April 1, 1997; 17(4): 778 - 785. [Abstract] [Full Text]

				R. E. Ostlund Jr., J. B. McGill, C.-M. Zeng, D. F. Covey, J. Stearns, W. F. Stenson, and C. A. Spilburg Gastrointestinal absorption and plasma kinetics of soy Delta 5-phytosterols and phytostanols in humans Am J Physiol Endocrinol Metab, April 1, 2002; 282(4): E911 - E916. [Abstract] [Full Text] [PDF]