Journal of Lipid Research, Vol 34, 943-959, Copyright © 1993 by Lipid Research, Inc.

ARTICLES

Plasma lipoproteins in the golden Syrian hamster (Mesocricetus auratus): heterogeneity of apoB- and apoA-I-containing particles

S Goulinet and MJ Chapman
Lipoprotein and Atherogenesis Research Unit, Institut National de la Sante et de la Recherche Medicale, Paris, France.

We present the quantitative and qualitative characteristics of the apolipoprotein (apo) B- and apoA-I-containing lipoprotein subspecies in the plasma of male Golden Syrian hamsters. The spectrum of hamster lipoproteins of d < 1.172 g/ml was subfractionated by isopycnic density gradient ultracentrifugation. ApoB-containing subspecies were distributed up to a density of 1.074 g/ml. Hamster very low density lipoproteins (VLDL, d < 1.018 g/ml; approximately 120 mg/dl plasma) were triglyceride (TG)-rich, deficient in cholesteryl ester (CE), and highly heterogeneous in size, containing chylomicron-like particles. ApoVLDL contained proteins analogous to human apoB-100, apoB-48, and apoE. ApoB-containing subspecies with physicochemical properties typical of low density lipoproteins (LDL) were identified as a single, major size species in the density interval from 1.019 to 1.074 g/ml, particle diameter decreasing progressively with increase in density. Hamster LDL-like subspecies were distinguished from their human counterparts by a relative deficiency in core CE (< 30% by wt) and by enrichment in triglyceride. The high M(r) form of apoB was the major apolipoprotein of all LDL-like subfractions, in which apoE was detected as a minor component. Total plasma levels of LDL (d 1.019-1.074 g/ml) amounted to approximately 140 mg/dl (approximately 25% of d < 1.172 g/ml lipoproteins). The distribution of dense apoB-containing subspecies overlapped that of apoA-I-containing, high density lipoprotein-1 (HDL1)-like particles in the density interval approximately 1.039-1.074 g/ml. ApoA-I-containing subspecies with the physical and chemical characteristics of HDL were exclusively present over the density interval 1.074-1.172 g/ml. Quantitatively, these subspecies predominated in hamster plasma (approximately 270 mg/dl). Light, HDL2-like particles of d 1.065-1.103 g/ml (HDLL) were preponderant, (approximately 66% of total HDL). Marked size heterogeneity was evident, and was associated with distinct particle contents of minor apolipoproteins. Both HDLL and heavy HDL (HDLH, d 1.103-1.172 g/ml) were enriched in CE as evidenced by elevated weight ratios of CE:FC (7-9:1) and of CE:TG (up to approximately 50:1). Considered together, the core lipid contents of apoB- and apoA-I- containing lipoproteins are consistent with the hypothesis that the hamster is partially deficient in neutral lipid (CE, TG) transfer activity.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				S. V. Gupta, N. Yamada, T. V. Fungwe, and P. Khosla Replacing 40% of Dietary Animal Fat with Vegetable Oil Is Associated with Lower HDL Cholesterol and Higher Cholesterol Ester Transfer Protein in Cynomolgus Monkeys Fed Sufficient Linoleic Acid J. Nutr., August 1, 2003; 133(8): 2600 - 2606. [Abstract] [Full Text] [PDF]

				J. Sher, A. Pronczuk, T. Hajri, and K. C. Hayes Dietary Conjugated Linoleic Acid Lowers Plasma Cholesterol during Cholesterol Supplementation, but Accentuates the Atherogenic Lipid Profile during the Acute Phase Response in Hamsters J. Nutr., February 1, 2003; 133(2): 456 - 460. [Abstract] [Full Text] [PDF]

				M. Haidari, N. Leung, F. Mahbub, K. D. Uffelman, R. Kohen-Avramoglu, G. F. Lewis, and K. Adeli Fasting and Postprandial Overproduction of Intestinally Derived Lipoproteins in an Animal Model of Insulin Resistance. EVIDENCE THAT CHRONIC FRUCTOSE FEEDING IN THE HAMSTER IS ACCOMPANIED BY ENHANCED INTESTINAL DE NOVO LIPOGENESIS AND ApoB48-CONTAINING LIPOPROTEIN OVERPRODUCTION J. Biol. Chem., August 23, 2002; 277(35): 31646 - 31655. [Abstract] [Full Text] [PDF]

				S. V. Gupta and P. Khosla Palmitic and Stearic Acids Similarly Affect Plasma Lipoprotein Metabolism in Cynomolgus Monkeys Fed Diets with Adequate Levels of Linoleic Acid J. Nutr., August 1, 2001; 131(8): 2115 - 2120. [Abstract] [Full Text] [PDF]

				S. V. Gupta and P. Khosla Pork Fat and Chicken Fat Similarly Affect Plasma Lipoprotein Metabolism in Cynomolgus Monkeys Fed Diets with Adequate Levels of Linoleic Acid J. Nutr., May 1, 2000; 130(5): 1217 - 1224. [Abstract] [Full Text]

				C. Taghibiglou, D. Rudy, S. C. Van Iderstine, A. Aiton, D. Cavallo, R. Cheung, and K. Adeli Intracellular mechanisms regulating apoB-containing lipoprotein assembly and secretion in primary hamster hepatocytes J. Lipid Res., March 1, 2000; 41(4): 499 - 513. [Abstract] [Full Text]

				A. Ibrahimi, A. Bonen, W. D. Blinn, T. Hajri, X. Li, K. Zhong, R. Cameron, and N. A. Abumrad Muscle-specific Overexpression of FAT/CD36 Enhances Fatty Acid Oxidation by Contracting Muscle, Reduces Plasma Triglycerides and Fatty Acids, and Increases Plasma Glucose and Insulin J. Biol. Chem., September 17, 1999; 274(38): 26761 - 26766. [Abstract] [Full Text] [PDF]

				D. K. Spady, D. M. Kearney, and H. H. Hobbs Polyunsaturated fatty acids up-regulate hepatic scavenger receptor B1 (SR-BI) expression and HDL cholesteryl ester uptake in the hamster J. Lipid Res., August 1, 1999; 40(8): 1384 - 1394. [Abstract] [Full Text]

				T. Hajri, P. Khosla, A. Pronczuk, and K. C. Hayes Myristic Acid-Rich Fat Raises Plasma LDL by Stimulating LDL Production without Affecting Fractional Clearance in Gerbils Fed a Cholesterol-Free Diet J. Nutr., March 1, 1998; 128(3): 477 - 484. [Abstract] [Full Text]

				S. Goulinet and M. J. Chapman Plasma LDL and HDL Subspecies Are Heterogenous in Particle Content of Tocopherols and Oxygenated and Hydrocarbon Carotenoids: Relevance to Oxidative Resistance and Atherogenesis Arterioscler. Thromb. Vasc. Biol., April 1, 1997; 17(4): 786 - 796. [Abstract] [Full Text]