Journal of Lipid Research, Vol 34, 1155-1163, Copyright © 1993 by Lipid Research, Inc.

ARTICLES

Lipoprotein lipase enhances the interaction of low density lipoproteins with artery-derived extracellular matrix proteoglycans

IJ Edwards, IJ Goldberg, JS Parks, H Xu and WD Wagner
Department of Comparative Medicine, Bowman Gray School of Medicine, Winston-Salem, NC 27157-1040.

The association of plasma low density lipoproteins (LDL) with arterial proteoglycans (PG) is of key importance in LDL retention and modification in the artery wall. Lipoprotein lipase (LpL), the rate- limiting enzyme for hydrolysis of lipoprotein triglyceride, is known to bind both LDL and arterial PG. In the presence of LpL, cellular internalization and degradation of LDL is enhanced by a pathway initiated by interaction of LDL with a cell surface heparan sulfate proteoglycan. To determine whether LpL enhances the binding of LDL to arterial chondroitin sulfate (CS)PG and dermatan sulfate (DS)PG, the major extracellular PG of the artery wall, a microtiter plate assay was used to study LpL-PG-LDL interactions. Binding of LDL to both CSPG and DSPG was increased in the presence of LpL but differential effects were seen for the two PG. LpL enhanced the binding of LDL to CSPG a maximum of 20% and to DSPG a maximum of 40%. Heparin displacement of PG binding suggested a greater binding strength for DSPG-LpL-LDL with 0.25 micrograms heparin required to displace 50% of DSPG compared to 0.01 micrograms to displace 50% of CSPG. The greater enhancement of DSPG-LDL interaction by LpL is of particular interest since increases in DSPG correlate with the accumulation of aortic cholesterol. These data suggest that lipoprotein lipase may enhance the interaction of plasma low density lipoprotein with arterial chondroitin sulfate proteoglycan and dermatan sulfate proteoglycan and thus facilitate low density lipoprotein retention in the artery wall.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				K. C-W. Yu, C. David, S. Kadambi, A. Stahl, K.-I. Hirata, T. Ishida, T. Quertermous, A. D. Cooper, and S. Y. Choi Endothelial lipase is synthesized by hepatic and aorta endothelial cells and its expression is altered in apoE-deficient mice J. Lipid Res., September 1, 2004; 45(9): 1614 - 1623. [Abstract] [Full Text] [PDF]

				K. Olin-Lewis, J. L. Benton, J. C. Rutledge, D. G. Baskin, T. N. Wight, and A. Chait Apolipoprotein E Mediates the Retention of High-Density Lipoproteins by Mouse Carotid Arteries and Cultured Arterial Smooth Muscle Cell Extracellular Matrices Circ. Res., June 28, 2002; 90(12): 1333 - 1339. [Abstract] [Full Text] [PDF]

				M. O. Pentikainen, R. Oksjoki, K. Oorni, and P. T. Kovanen Lipoprotein Lipase in the Arterial Wall: Linking LDL to the Arterial Extracellular Matrix and Much More Arterioscler. Thromb. Vasc. Biol., February 1, 2002; 22(2): 211 - 217. [Abstract] [Full Text] [PDF]

				K. Wilson, G. L. Fry, D. A. Chappell, C. D. Sigmund, and J. D. Medh Macrophage-Specific Expression of Human Lipoprotein Lipase Accelerates Atherosclerosis in Transgenic Apolipoprotein E Knockout Mice but Not in C57BL/6 Mice Arterioscler. Thromb. Vasc. Biol., November 1, 2001; 21(11): 1809 - 1815. [Abstract] [Full Text] [PDF]

				R. Zimmermann, U. Panzenbock, A. Wintersperger, S. Levak-Frank, W. Graier, O. Glatter, G. Fritz, G. M. Kostner, and R. Zechner Lipoprotein Lipase Mediates the Uptake of Glycated LDL in Fibroblasts, Endothelial Cells, and Macrophages Diabetes, July 1, 2001; 50(7): 1643 - 1653. [Abstract] [Full Text] [PDF]

				B. Hennig, M. Toborek, and C. J. McClain High-Energy Diets, Fatty Acids and Endothelial Cell Function: Implications for Atherosclerosis J. Am. Coll. Nutr., April 1, 2001; 20(2): 97 - 105. [Abstract] [Full Text] [PDF]

				M. Van Eck, R. Zimmermann, P. H. E. Groot, R. Zechner, and T. J. C. Van Berkel Role of Macrophage-Derived Lipoprotein Lipase in Lipoprotein Metabolism and Atherosclerosis Arterioscler. Thromb. Vasc. Biol., September 1, 2000; 20 (9): e53 - e62. [Abstract] [Full Text] [PDF]

				S. M. Clee, N. Bissada, F. Miao, L. Miao, A. D. Marais, H. E. Henderson, P. Steures, J. McManus, B. McManus, R. C. LeBoeuf, et al. Plasma and vessel wall lipoprotein lipase have different roles in atherosclerosis J. Lipid Res., April 1, 2000; 41(4): 521 - 531. [Abstract] [Full Text]

				K. L. Olin, S. Potter-Perigo, P. H. R. Barrett, T. N. Wight, and A. Chait Lipoprotein Lipase Enhances the Binding of Native and Oxidized Low Density Lipoproteins to Versican and Biglycan Synthesized by Cultured Arterial Smooth Muscle Cells J. Biol. Chem., December 3, 1999; 274(49): 34629 - 34636. [Abstract] [Full Text] [PDF]

				S. Marathe, G. Kuriakose, K. J. Williams, and I. Tabas Sphingomyelinase, an Enzyme Implicated in Atherogenesis, Is Present in Atherosclerotic Lesions and Binds to Specific Components of the Subendothelial Extracellular Matrix Arterioscler. Thromb. Vasc. Biol., November 1, 1999; 19(11): 2648 - 2658. [Abstract] [Full Text] [PDF]

				N. P. Dantuma, M. A. P. Pijnenburg, J. H. B. Diederen, and D. J. Van der Horst Multiple interactions between insect lipoproteins and fat body cells: extracellular trapping and endocytic trafficking J. Lipid Res., September 1, 1998; 39(9): 1877 - 1888. [Abstract] [Full Text]

				M. Y. Chang, K. L. Olin, C. Tsoi, T. N. Wight, and A. Chait Human Monocyte-derived Macrophages Secrete Two Forms of Proteoglycan-Macrophage Colony-stimulating Factor That Differ in Their Ability to Bind Low Density Lipoproteins J. Biol. Chem., June 26, 1998; 273(26): 15985 - 15992. [Abstract] [Full Text] [PDF]

				C. S. Moran, J. H. Campbell, and G. R. Campbell Human Leukemia Inhibitory Factor Upregulates LDL Receptors on Liver Cells and Decreases Serum Cholesterol in the Cholesterol-Fed Rabbit Arterioscler. Thromb. Vasc. Biol., July 1, 1997; 17(7): 1267 - 1273. [Abstract] [Full Text]

				U. Olsson, G. Camejo, E. Hurt-Camejo, K. Elfsber, O. Wiklund, and G. Bondjers Possible Functional Interactions of Apolipoprotein B-100 Segments That Associate With Cell Proteoglycans and the ApoB/E Receptor Arterioscler. Thromb. Vasc. Biol., January 1, 1997; 17(1): 149 - 155. [Abstract] [Full Text]

				I. J. Edwards, H. Xu, J. C. Obunike, I. J. Goldberg, and W. D. Wagner Differentiated Macrophages Synthesize a Heparan Sulfate Proteoglycan and an Oversulfated Chondroitin Sulfate Proteoglycan That Bind Lipoprotein Lipase Arterioscler. Thromb. Vasc. Biol., March 1, 1995; 15(3): 400 - 409. [Abstract] [Full Text]

				J. Boren, A. Lookene, E. Makoveichuk, S. Xiang, M. Gustafsson, H. Liu, P. Talmud, and G. Olivecrona Binding of Low Density Lipoproteins to Lipoprotein Lipase Is Dependent on Lipids but Not on Apolipoprotein B J. Biol. Chem., July 13, 2001; 276(29): 26916 - 26922. [Abstract] [Full Text] [PDF]