Journal of Lipid Research, Vol 35, 1902-1906, Copyright © 1994 by Lipid Research, Inc.
Conditions for solid-phase extraction of urinary bile acids with octadecylsilane-bonded silica
E Wahlen, N Tamasawa, H Ichimiya, B Egestad and J Sjovall
Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden.
Fast atom bombardment mass spectrometry was used as a detection method in a
study of the extraction of urinary bile acids with octadecylsilane- bonded
silica. The procedure commonly used in many laboratories was found to
result in significant losses of sulfated taurine-conjugated bile acids.
Losses of other double conjugates and of bile acid and bile alcohol
glucuronides were also seen. The losses were avoided by addition of an
equal volume of 0.5 M triethylamine sulfate to the urine before passing it
through the sorbent bed. Quantitative elution was then achieved with
methanol. Batch variations were observed with sorbents from two different
manufacturers.
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