Journal of Lipid Research, Vol 35, 1993-2007, Copyright © 1994 by Lipid Research, Inc.

ARTICLES

Effects of increasing amounts of dietary cholesterol on postprandial lipemia and lipoproteins in human subjects

C Dubois, M Armand, N Mekki, H Portugal, AM Pauli, PM Bernard, H Lafont and D Lairon
Unite 130-INSERM (National Institute of Health and Medical Research), Marseille, France.

Our aim was to determine the effects of increasing amounts of dietary cholesterol (0-710 mg) on the postprandial plasma lipid responses and lipoprotein changes in normolipidemic human subjects. Ten subjects were fed five different test meals in a random order: one meal did not contain fat or cholesterol while the four others contained a fixed amount of lipids (45 g) and 0, 140, 280, and 710 mg cholesterol, respectively. Fasting and post-meal blood samples were obtained for 7 h. Large and small triglyceride-rich lipoproteins (TRL), low density (LDL), and high density (HDL) lipoproteins were isolated. Compared to the no-fat, no-cholesterol meal, the fat-enriched meals raised (P < 0.05) plasma triglycerides, phospholipids, and free cholesterol and lowered cholesteryl esters postprandially. The meals containing zero or 140 mg cholesterol generally elicited comparable postprandial plasma and lipoprotein lipid responses. The meals providing 280 or 710 mg cholesterol significantly increased postprandial plasma phospholipids and large TRL triglycerides and decreased plasma esterified cholesterol. The lipid composition of the large TRLs and the concentrations of the small TRL lipid components were not altered postprandially by cholesterol intake. On the other hand, LDL free cholesterol increased after 3 h, LDL cholesteryl esters dropped after 3 and 7 h, HDL cholesteryl esters dropped after 3 h, and HDL phospholipids increased 7 h after ingesting meals highly enriched in cholesterol. Blood insulin, apoA-I and apoB were not altered postprandially by cholesterol intake. Thus, the data show that ingesting more than 140 mg cholesterol per meal significantly alters the postprandial lipoprotein response in healthy subjects.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				S. S Lund, L. Tarnow, M. Frandsen, U. M Smidt, O. Pedersen, H.-H. Parving, and A. A Vaag Impact of metformin versus the prandial insulin secretagogue, repaglinide, on fasting and postprandial glucose and lipid responses in non-obese patients with type 2 diabetes Eur. J. Endocrinol., January 1, 2008; 158(1): 35 - 46. [Abstract] [Full Text] [PDF]

				H.-Y. Chung, H. M. Rasmussen, and E. J. Johnson Lutein Bioavailability Is Higher from Lutein-Enriched Eggs than from Supplements and Spinach in Men J. Nutr., August 1, 2004; 134(8): 1887 - 1893. [Abstract] [Full Text] [PDF]

				N. Mekki, M. Charbonnier, P. Borel, J. Leonardi, C. Juhel, H. Portugal, and D. Lairon Butter Differs from Olive Oil and Sunflower Oil in Its Effects on Postprandial Lipemia and Triacylglycerol-Rich Lipoproteins after Single Mixed Meals in Healthy Young Men J. Nutr., December 1, 2002; 132(12): 3642 - 3649. [Abstract] [Full Text] [PDF]

				C. Marin, J. Lopez-Miranda, P. Gomez, E. Paz, P. Perez-Martinez, F. Fuentes, J. A. Jimenez-Pereperez, J. M. Ordovas, and F. Perez-Jimenez Effects of the human apolipoprotein A-I promoter G-A mutation on postprandial lipoprotein metabolism Am. J. Clinical Nutrition, August 1, 2002; 76(2): 319 - 325. [Abstract] [Full Text] [PDF]

				G. Beaumier-Gallon, C. Dubois, M. Senft, M.-F. Vergnes, A.-M. Pauli, H. Portugal, and D. Lairon Dietary cholesterol is secreted in intestinally derived chylomicrons during several subsequent postprandial phases in healthy humans Am. J. Clinical Nutrition, May 1, 2001; 73(5): 870 - 877. [Abstract] [Full Text] [PDF]

				X. Hu, R. J Jandacek, and W. S White Intestinal absorption of {beta}-carotene ingested with a meal rich in sunflower oil or beef tallow: postprandial appearance in triacylglycerol-rich lipoproteins in women Am. J. Clinical Nutrition, May 1, 2000; 71(5): 1170 - 1180. [Abstract] [Full Text] [PDF]

				H. Jacques, A. Gascon, J. Arul, A. Boudreau, C. Lavigne, and J. Bergeron Modified milk fat reduces plasma triacylglycerol concentrations in normolipidemic men compared with regular milk fat and nonhydrogenated margarine Am. J. Clinical Nutrition, December 1, 1999; 70(6): 983 - 991. [Abstract] [Full Text] [PDF]

				M. Armand, B. Pasquier, M. Andre, P. Borel, M. Senft, J. Peyrot, J. Salducci, H. Portugal, V. Jaussan, and D. Lairon Digestion and absorption of 2 fat emulsions with different droplet sizes in the human digestive tract Am. J. Clinical Nutrition, December 1, 1999; 70(6): 1096 - 1106. [Abstract] [Full Text] [PDF]

				M. J. Singleton, C. Heiser, K. Jamesen, and R. D. Mattes Sweetener Augmentation of Serum Triacylglycerol during a Fat Challenge Test in Humans J. Am. Coll. Nutr., April 1, 1999; 18(2): 179 - 185. [Abstract] [Full Text] [PDF]

				M A. Zulet, A. Barber, H. Garcin, P. Higueret, and J. A. Martinez Alterations in Carbohydrate and Lipid Metabolism Induced by a Diet Rich in Coconut Oil and Cholesterol in a Rat Model J. Am. Coll. Nutr., February 1, 1999; 18(1): 36 - 42. [Abstract] [Full Text] [PDF]

				J. Lopez-Miranda, J.M. Ordovas, M.A. Ostos, C. Marin, S. Jansen, J. Salas, A. Blanco-Molina, J.A. Jimenez-Pereperez, F. Lopez-Segura, and F. Perez-Jimenez Dietary Fat Clearance in Normal Subjects Is Modulated by Genetic Variation at the Apolipoprotein B Gene Locus Arterioscler. Thromb. Vasc. Biol., September 1, 1997; 17(9): 1765 - 1773. [Abstract] [Full Text]

				N. Bergeron and R. J. Havel Influence of Diets Rich in Saturated and Omega-6 Polyunsaturated Fatty Acids on the Postprandial Responses of Apolipoproteins B-48, B-100, E, and Lipids in Triglyceride-Rich Lipoproteins Arterioscler. Thromb. Vasc. Biol., December 1, 1995; 15(12): 2111 - 2121. [Abstract] [Full Text]