Journal of Lipid Research, Vol 35, 255-262, Copyright © 1994 by Lipid Research, Inc.

ARTICLES

Incorporation of 13-hydroxyoctadecadienoic acid (13-HODE) into epidermal ceramides and phospholipids: phospholipase C-catalyzed release of novel 13-HODE-containing diacylglycerol

Y Cho and VA Ziboh
Department of Dermatology, University of California, Davis 95616.

Ceramides and phospholipids constitute two important structural lipids of normal skin that are notably rich in polyunsaturated fatty acids. Although linoleic acid (LA) is high in the ceramides, the localization of its 15-lipoxygenase product, 13-hydroxyoctadecadienoic acid (13- HODE) in the epidermis is unknown. In this study, we investigated the relative incorporation of [14C]LA and [14C]13-HODE into ceramides and phospholipids in isolated epidermal slices. Our data revealed minor incorporation of [14C]LA and [14C]13-HODE into ceramides. In contrast, both [14C]LA and [14C]13-HODE are markedly incorporated into phospholipids, particularly, phosphatidylcholine (PC) and phosphatidylinositol (PtdIns). The incorporation of 13-HODE into the PtdIns pool in particular prompted us to investigate into its fate in the signal transduction process and its possible incorporation into diacylglycerol. Our data revealed that 13-HODE is incorporated into epidermal phosphatidylinositol 4,5-bisphosphate (PtdIns4,5-P2) resulting in epidermal phospholipase C-catalyzed release into a novel 13-HODE-containing diacylglycerol (1-acyl-2-13-HODE-glycerol). The possibility now exists that this novel 13-HODE-containing diacylglycerol could function to modulate the activity of epidermal protein kinase C and hyperproliferation/differentiation.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				S. Chung, S. Kong, K. Seong, and Y. Cho {gamma}-Linolenic Acid in Borage Oil Reverses Epidermal Hyperproliferation in Guinea Pigs J. Nutr., October 1, 2002; 132(10): 3090 - 3097. [Abstract] [Full Text] [PDF]

				A. R. Jude, J. M. Little, A. W. Bull, I. Podgorski, and A. Radominska-Pandya 13-Hydroxy- and 13-Oxooctadecadienoic acids: Novel Substrates for Human UDP-Glucuronosyltransferases Drug Metab. Dispos., April 13, 2001; 29(5): 652 - 655. [Abstract] [Full Text]

				V. A Ziboh, C. C Miller, and Y. Cho Metabolism of polyunsaturated fatty acids by skin epidermal enzymes: generation of antiinflammatory and antiproliferative metabolites1 Am. J. Clinical Nutrition, January 1, 2000; 71(1): 361S - 366S. [Abstract] [Full Text] [PDF]

				X. Fang, T. L. Kaduce, and A. A. Spector 13-(S)-Hydroxyoctadecadienoic acid (13-HODE) incorporation and conversion to novel products by endothelial cells J. Lipid Res., April 1, 1999; 40(4): 699 - 707. [Abstract] [Full Text]