J. Lipid Res. Did you know there is a large type edition? Click here.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Une, M.
Right arrow Articles by Hoshita, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Une, M.
Right arrow Articles by Hoshita, T.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Journal of Lipid Research, Vol 35, 620-624, Copyright © 1994 by Lipid Research, Inc.

ARTICLES

Identification of (24E)-3 alpha,7 alpha-dihydroxy-5 beta-cholest-24- enoic acid and (24R,25S)-3 alpha,7 alpha,24-trihydroxy-5 beta- cholestanoic acid as intermediates in the conversion of 3 alpha,7 alpha- dihydroxy-5 beta-cholestanoic acid to chenodeoxycholic acid in rat liver homogenates

M Une, A Inoue, T Kurosawa, M Tohma and T Hoshita
Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine, Japan.

Studies of chemical structure of the intermediates in the biosynthetic sequence between 3 alpha,7 alpha-dihydroxy-5 beta-cholestanoic acid (DHCA) and chenodeoxycholic acid have been undertaken. Radiolabeled DHCA was incubated with a rat liver preparation. The reaction products were converted to the p-bromophenacyl esters, and analyzed by reversed- phase high performance liquid chromatography. Under the conditions used, the radioactivity was found in (24E)-3 alpha,7 alpha-dihydroxy-5 beta-cholest-24-enoic acid (31%) and (24R,25S)-3 alpha,7 alpha,24- trihydroxy-5 beta-cholestanoic acid (7%) along with the starting material (62%). Neither the 24Z isomer of the alpha,beta-unsaturated bile acid nor the other three isomers of the beta-hydroxy bile acid were detected. The findings support the proposed pathway for the side chain cleavage in chenodeoxycholic acid biosynthesis, which is thought to be identical to that of cholic acid biosynthesis.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Lipid Res.Home page
T. Nishimaki-Mogami, M. Une, T. Fujino, Y. Sato, N. Tamehiro, Y. Kawahara, K. Shudo, and K. Inoue
Identification of intermediates in the bile acid synthetic pathway as ligands for the farnesoid X receptor
J. Lipid Res., August 1, 2004; 45(8): 1538 - 1545.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
M. Une, Y. Iguchi, T. Sakamoto, T. Tomita, Y. Suzuki, M. Morita, and T. Imanaka
ATP-Dependent Transport of Bile Acid Intermediates across Rat Liver Peroxisomal Membranes
J. Biochem., August 1, 2003; 134(2): 225 - 230.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 1994 by the American Society for Biochemistry and Molecular Biology.