Journal of Lipid Research, Vol 35, 1318-1328, Copyright © 1994 by Lipid Research, Inc.

ARTICLES

Effect of sample storage on the measurement of lipoprotein[a], apolipoproteins B and A-IV, total and high density lipoprotein cholesterol and triglycerides

F Kronenberg, EM Lobentanz, P Konig, G Utermann and H Dieplinger
Institute of Medical Biology and Human Genetics, Innsbruck, Austria.

This study investigated the influence of long-term storage, for periods up to 24 months, and multiple freezing and thawing on the measured values of lipoprotein[a] (Lp[a]), apolipoproteins B and A-IV, total and high density lipoprotein (HDL) cholesterol and triglycerides using plasma samples stored at -80 degrees C, -20 degrees C, and 4 degrees C. Samples stored at -80 degrees C or -20 degrees C showed significant changes in Lp[a] after 24 months, with a mean decrease of 7% and 13%, respectively (P < 0.01). The major part of the decrease occurred during the first freezing and thawing. In contrast, apolipoproteins B and A-IV decreased continuously over time (P < 0.05). The increase in plasma concentrations of total and HDL cholesterol and triglycerides was small but significant because of its uniformity. Multiple freezing and thawing influenced only the measured values of Lp[a] and apolipoprotein B. Comparison of samples stored at -80 degrees C and -20 degrees C showed no difference in any of the parameters at any time with the exception of Lp[a] after 18 and 24 months (P < 0.05). After a storage period of 24 months, immunoblotting with detection of apo[a] was possible from samples under each storage condition. ApoB and apoA-IV were detectable only in samples stored at -20 degrees C or -80 degrees C. These data, when compared to recent studies, suggest a critical role of the assay methodology in the reproducibility of measured Lp[a] and apolipoprotein plasma concentrations. We therefore recommend the examination of each system for measurement of long-term stored plasma samples.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				A. E Cust, R. Kaaks, C. Friedenreich, F. Bonnet, M. Laville, A. Tjonneland, A. Olsen, K. Overvad, M. U. Jakobsen, V. Chajes, et al. Metabolic syndrome, plasma lipid, lipoprotein and glucose levels, and endometrial cancer risk in the European Prospective Investigation into Cancer and Nutrition (EPIC) Endocr. Relat. Cancer, September 1, 2007; 14(3): 755 - 767. [Abstract] [Full Text] [PDF]

				B. Dieplinger, A. Lingenhel, N. Baumgartner, W. Poelz, H. Dieplinger, M. Haltmayer, F. Kronenberg, and T. Mueller Increased Serum Lipoprotein(a) Concentrations and Low Molecular Weight Phenotypes of Apolipoprotein(a) Are Associated with Symptomatic Peripheral Arterial Disease Clin. Chem., July 1, 2007; 53(7): 1298 - 1305. [Abstract] [Full Text] [PDF]

				B. Kollerits, M. Auinger, V. Reisig, T. Kastenbauer, A. Lingenhel, K. Irsigler, R. Prager, and F. Kronenberg Lipoprotein(a) as a Predictor of Cardiovascular Disease in a Prospectively Followed Cohort of Patients With Type 1 Diabetes Diabetes Care, July 1, 2006; 29(7): 1661 - 1663. [Full Text] [PDF]

				E. Boes, D. Fliser, E. Ritz, P. Konig, K. Lhotta, J. F.E. Mann, G. A. Muller, U. Neyer, W. Riegel, P. Riegler, et al. Apolipoprotein A-IV Predicts Progression of Chronic Kidney Disease: The Mild to Moderate Kidney Disease Study J. Am. Soc. Nephrol., February 1, 2006; 17(2): 528 - 536. [Abstract] [Full Text] [PDF]

				A. G. Rundle, P. Vineis, and H. Ahsan Design Options for Molecular Epidemiology Research within Cohort Studies Cancer Epidemiol. Biomarkers Prev., August 1, 2005; 14(8): 1899 - 1907. [Abstract] [Full Text] [PDF]

				J. S. Cohn, C. Rodriguez, H. Jacques, M. Tremblay, and J. Davignon Storage of human plasma samples leads to alterations in the lipoprotein distribution of apoC-III and apoE J. Lipid Res., August 1, 2004; 45(8): 1572 - 1579. [Abstract] [Full Text] [PDF]

				B. Ezeh, M. Haiman, H. F. Alber, B. Kunz, B. Paulweber, A. Lingenhel, H.-G. Kraft, F. Weidinger, O. Pachinger, H. Dieplinger, et al. Plasma distribution of apoA-IV in patients with coronary artery disease and healthy controls J. Lipid Res., August 1, 2003; 44(8): 1523 - 1529. [Abstract] [Full Text] [PDF]

				M. Kratz, U. Wahrburg, A. von Eckardstein, B. Ezeh, G. Assmann, and F. Kronenberg Dietary Mono- and Polyunsaturated Fatty Acids Similarly Increase Plasma Apolipoprotein A-IV Concentrations in Healthy Men and Women J. Nutr., June 1, 2003; 133(6): 1821 - 1825. [Abstract] [Full Text] [PDF]

				F. Kronenberg, E. Kuen, E. Ritz, P. Konig, G. Kraatz, K. Lhotta, J. F. E. Mann, G. A. Muller, U. Neyer, W. Riegel, et al. Apolipoprotein A-IV Serum Concentrations Are Elevated in Patients with Mild and Moderate Renal Failure J. Am. Soc. Nephrol., February 1, 2002; 13(2): 461 - 469. [Abstract] [Full Text] [PDF]

				B. Garner, D. J. Harvey, L. Royle, M. Frischmann, F. Nigon, M. J. Chapman, and P. M. Rudd Characterization of human apolipoprotein B100 oligosaccharides in LDL subfractions derived from normal and hyperlipidemic plasma: deficiency of {alpha}-N-acetylneuraminyllactosyl-ceramide in light and small dense LDL particles Glycobiology, October 1, 2001; 11(10): 791 - 802. [Abstract] [Full Text] [PDF]

				J. M. Simo, J. Camps, E. Vilella, F. Gomez, A. Paul, and J. Joven Instability of Lipoprotein(a) in Plasma Stored at -70 {degrees}C : Effects of Concentration, Apolipoprotein(a) Genotype, and Donor Cardiovascular Disease Clin. Chem., September 1, 2001; 47(9): 1673 - 1678. [Abstract] [Full Text] [PDF]

				F. Kronenberg, M. Stuhlinger, E. Trenkwalder, F. S. Geethanjali, O. Pachinger, A. von Eckardstein, and H. Dieplinger Low apolipoprotein A-IV plasma concentrations in men with coronary artery disease J. Am. Coll. Cardiol., September 1, 2000; 36(3): 751 - 757. [Abstract] [Full Text] [PDF]

				W. J. Shih, P. S. Bachorik, J. A. Haga, G. L. Myers, and E. A. Stein Estimating the Long-Term Effects of Storage at -70 {degrees}C on Cholesterol, Triglyceride, and HDL-Cholesterol Measurements in Stored Sera Clin. Chem., March 1, 2000; 46(3): 351 - 364. [Abstract] [Full Text] [PDF]

				F. KRONENBERG, E. KUEN, E. RITZ, R. JUNKER, P. KÖNIG, G. KRAATZ, K. LHOTTA, J. F. E. MANN, G. A. MÜLLER, U. NEYER, et al. Lipoprotein(a) Serum Concentrations and Apolipoprotein(a) Phenotypes in Mild and Moderate Renal Failure J. Am. Soc. Nephrol., January 1, 2000; 11(1): 105 - 115. [Abstract] [Full Text]

				F. KRONENBERG, U. NEYER, K. LHOTTA, E. TRENKWALDER, M. AUINGER, A. PRIBASNIG, T. MEISL, P. KÖNIG, and H. DIEPLINGER The Low Molecular Weight Apo(a) Phenotype Is an Independent Predictor for Coronary Artery Disease in Hemodialysis Patients: A ProspectiveFollow-Up J. Am. Soc. Nephrol., May 1, 1999; 10(5): 1027 - 1036. [Abstract] [Full Text]

				P. Fontana, V. Mooser, P. Bovet, C. Shamlaye, B. Burnand, V. Lenain, S. M. Marcovina, W. Riesen, and R. Darioli Dose-Dependent Inverse Relationship Between Alcohol Consumption and Serum Lp(a) Levels in Black African Males Arterioscler. Thromb. Vasc. Biol., April 1, 1999; 19(4): 1075 - 1082. [Abstract] [Full Text] [PDF]

				P. N. Hopkins, L. L. Wu, S. C. Hunt, B. C. James, G. M. Vincent, and R. R. Williams Lipoprotein(a) Interactions With Lipid and Nonlipid Risk Factors in Early Familial Coronary Artery Disease Arterioscler. Thromb. Vasc. Biol., November 1, 1997; 17(11): 2783 - 2792. [Abstract] [Full Text]

				C. Brunner, E.-M. Lobentanz, A. Petho-Schramm, A. Ernst, C. Kang, H. Dieplinger, H.-J. Muller, and G. Utermann The Number of Identical Kringle IV Repeats in Apolipoprotein(a) Affects Its Processing and Secretion by HepG2 Cells J. Biol. Chem., December 13, 1996; 271(50): 32403 - 32410. [Abstract] [Full Text] [PDF]

				F. Kronenberg, E. Trenkwalder, H. Dieplinger, and G. Utermann Lipoprotein(a) in Stored Plasma Samples and the Ravages of Time: Why Epidemiological Studies Might Fail Arterioscler. Thromb. Vasc. Biol., December 1, 1996; 16(12): 1568 - 1572. [Abstract] [Full Text]

				J. Willeit, S. Kiechl, P. Santer, F. Oberhollenzer, G. Egger, E. Jarosch, and A. Mair Lipoprotein(a) and Asymptomatic Carotid Artery Disease : Evidence of a Prominent Role in the Evolution of Advanced Carotid Plaques: The Bruneck Study Stroke, September 1, 1995; 26(9): 1582 - 1587. [Abstract] [Full Text]