Journal of Lipid Research, Vol 35, 1378-1385, Copyright © 1994 by Lipid Research, Inc.

ARTICLES

Distribution of angiotensin II receptors in rat and human adipocytes

DL Crandall, HE Herzlinger, BD Saunders, DC Armellino and JG Kral
Departments of Medicine, SUNY Health Science Center, Brooklyn.

Angiotensin II (AII) receptor binding assays were performed in rat adipocytes from three separate anatomic depots. Fat cells were isolated by collagenase digestion, and plasma membranes were prepared from the epididymal, mesenteric, and retroperitoneal fat depots of male Sprague- Dawley rats at 100 days of age. Binding of 125I-labeled [Sar1,Ile8]AII was rapid, saturable, and specific in membranes from all depots, identifying a receptor with a similar affinity of approximately 1 nM. Site-associated differences in receptor number were observed, with epididymal and mesenteric fat cell membranes exhibiting significantly more receptors than retroperitoneal fat cells when binding was expressed per unit of membrane protein. When corrected for cell volume, the number of receptors per cell ranked epididymal > retroperitoneal > mesenteric. Inhibitory constants for the peptide agonists AII and AIII and the peptide antagonist [Sar1,Ala8]AII indicated similar affinities in all three depots. Because the receptor has been classified pharmacologically into two subtypes, the AT1 selective antagonist losartan, and the AT2 selective antagonist PD 123,319 were used to classify the adipocyte receptor, indicating an AT1 subtype with an affinity for losartan in the mesenteric and retroperitoneal adipocytes that was significantly greater than the epididymal. Similar studies were performed in adipocyte membranes obtained from human omental and subcutaneous adipose tissue, revealing the presence of an AII receptor in both depots with an affinity of approximately 10 nM for losartan. These data indicate site-specific differences in AII receptor number in fat cell membranes from rats and the existence of human adipocyte AII receptors, suggesting that the adipocyte is significant for the peripheral metabolism of components of the renin-angiotensin system.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. B. Zemel and X. Sun Dietary Calcium and Dairy Products Modulate Oxidative and Inflammatory Stress in Mice and Humans J. Nutr., June 1, 2008; 138(6): 1047 - 1052. [Abstract] [Full Text] [PDF]

				N. O. Belo, M. R. Sairam, and A. M. dos Reis Impairment of the Natriuretic Peptide System in Follitropin Receptor Knockout Mice and Reversal by Estradiol: Implications for Obesity-Associated Hypertension in Menopause Endocrinology, March 1, 2008; 149(3): 1399 - 1406. [Abstract] [Full Text] [PDF]

				D. L. Crandall, E. M. Quinet, S. El Ayachi, A. L. Hreha, C. E. Leik, D. A. Savio, I. Juhan-Vague, and M.-C. Alessi Modulation of Adipose Tissue Development by Pharmacological Inhibition of PAI-1 Arterioscler. Thromb. Vasc. Biol., October 1, 2006; 26(10): 2209 - 2215. [Abstract] [Full Text] [PDF]

				M. Paul, A. Poyan Mehr, and R. Kreutz Physiology of local Renin-Angiotensin systems. Physiol Rev, July 1, 2006; 86(3): 747 - 803. [Abstract] [Full Text] [PDF]

				C. S. Carter, G. Onder, S. B. Kritchevsky, and M. Pahor Angiotensin-Converting Enzyme Inhibition Intervention in Elderly Persons: Effects on Body Composition and Physical Performance J. Gerontol. A Biol. Sci. Med. Sci., November 1, 2005; 60(11): 1437 - 1446. [Abstract] [Full Text] [PDF]

				C. M Boustany, D. R. Brown, D. C. Randall, and L. A Cassis AT1-receptor antagonism reverses the blood pressure elevation associated with diet-induced obesity Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2005; 289(1): R181 - R186. [Abstract] [Full Text] [PDF]

				C. M. Boustany, K. Bharadwaj, A. Daugherty, D. R. Brown, D. C. Randall, and L. A. Cassis Activation of the systemic and adipose renin-angiotensin system in rats with diet-induced obesity and hypertension Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2004; 287(4): R943 - R949. [Abstract] [Full Text] [PDF]

				T. Skurk, V. van Harmelen, and H. Hauner Angiotensin II Stimulates the Release of Interleukin-6 and Interleukin-8 From Cultured Human Adipocytes by Activation of NF-{kappa}B Arterioscler. Thromb. Vasc. Biol., July 1, 2004; 24(7): 1199 - 1203. [Abstract] [Full Text] [PDF]

				C. S. Carter, M. Cesari, W. T. Ambrosius, N. Hu, D. Diz, S. Oden, W. E. Sonntag, and M. Pahor Angiotensin-Converting Enzyme Inhibition, Body Composition, and Physical Performance in Aged Rats J. Gerontol. A Biol. Sci. Med. Sci., May 1, 2004; 59(5): B416 - B423. [Abstract] [Full Text] [PDF]

				V. Serazin, M.-N. Dieudonne, M. Morot, P. de Mazancourt, and Y. Giudicelli cAMP-positive regulation of angiotensinogen gene expression and protein secretion in rat adipose tissue Am J Physiol Endocrinol Metab, March 1, 2004; 286(3): E434 - E438. [Abstract] [Full Text] [PDF]

				L. A. Cassis, V. L. English, K. Bharadwaj, and C. M. Boustany Differential Effects of Local Versus Systemic Angiotensin II in the Regulation of Leptin Release from Adipocytes Endocrinology, January 1, 2004; 145(1): 169 - 174. [Abstract] [Full Text] [PDF]

				T. Skurk, Y.-M. Lee, and H. Hauner Angiotensin II and Its Metabolites Stimulate PAI-1 Protein Release From Human Adipocytes in Primary Culture Hypertension, May 1, 2001; 37(5): 1336 - 1340. [Abstract] [Full Text] [PDF]

				P. Saint-Marc, L. P. Kozak, G. Ailhaud, C. Darimont, and R. Negrel Angiotensin II as a Trophic Factor of White Adipose Tissue: Stimulation of Adipose Cell Formation Endocrinology, January 1, 2001; 142(1): 487 - 492. [Abstract] [Full Text] [PDF]

				V. Serazin-Leroy, M. Morot, P. de Mazancourt, and Y. Giudicelli Androgen regulation and site specificity of angiotensinogen gene expression and secretion in rat adipocytes Am J Physiol Endocrinol Metab, December 1, 2000; 279(6): E1398 - E1405. [Abstract] [Full Text] [PDF]

				S. Engeli, R. Negrel, and A. M. Sharma Physiology and Pathophysiology of the Adipose Tissue Renin-Angiotensin System Hypertension, June 1, 2000; 35(6): 1270 - 1277. [Abstract] [Full Text] [PDF]

				D. L. Crandall, D. C. Armellino, D. E. Busler, B. McHendry-Rinde, and J. G. Kral Angiotensin II Receptors in Human Preadipocytes: Role in Cell Cycle Regulation Endocrinology, January 1, 1999; 140(1): 154 - 158. [Abstract] [Full Text]

				C. Karlsson, K. Lindell, M. Ottosson, L. Sjöström, B. Carlsson, and L. M. S. Carlsson Human Adipose Tissue Expresses Angiotensinogen and Enzymes Required for Its Conversion to Angiotensin II J. Clin. Endocrinol. Metab., November 1, 1998; 83(11): 3925 - 3929. [Abstract] [Full Text]

				B. H. Jones, M. K. Standridge, and N. Moustaid Angiotensin II Increases Lipogenesis in 3T3-L1 and Human Adipose Cells Endocrinology, April 1, 1997; 138(4): 1512 - 1519. [Abstract] [Full Text] [PDF]

				T. L. Goodfriend, M. E. Elliott, and K. J. Catt Angiotensin Receptors and Their Antagonists N. Engl. J. Med., June 20, 1996; 334(25): 1649 - 1655. [Full Text] [PDF]