|
 |
|
|||||||
|
|||||||||
Journal of Lipid Research, Vol 36, 13-24, Copyright © 1995 by Lipid Research, Inc.
MM van Greevenbroek, WF Voorhout, DW Erkelens, G van Meer and TW de Bruin
Polarized monolayers of intestinal Caco-2 cells were used to study the
effects of saturated palmitic acid (16:0) and polyunsaturated linoleic acid
(18:2) on triglyceride synthesis and lipoprotein secretion. Monolayers were
incubated for 24 h, at the apical or lumenal side, with palmitic acid
(16:0) or linoleic acid (18:2) in physiological concentrations. Incubation
with 1.0 mM 16:0 or 18:2 resulted in differences in the composition and
amount of secreted lipoproteins. Radiolabeled lipids in the lipoproteins
secreted during incubation with 18:2 were found in the chylomicron/VLDL
(very low density lipoprotein) density whereas with 16:0 the secreted
lipoproteins were in the intermediate density/low density lipoprotein
(IDL/LDL) density range. More triglyceride was secreted into the
(basolateral) medium during incubation with 1.0 mM 18:2 (41 +/- 12% of
total triglyceride synthesized) than with 1.0 mM 16:0 (18 +/- 3% of total).
The biochemical findings correlate with conspicuous morphological changes
in the cells in the presence of 16:0, but not 18:2. Increasing
concentrations of 16:0 (0.1-1.0 mM) caused gradual accumulation of
intracellular membrane. Microvilli became strongly reduced in number. With
1.0 mM palmitic acid we found an increased incorporation of [1-
14C]palmitic acid into phosphatidic acid (14.8% of total incorporation into
phospholipid with 16:0 vs. < 0.5% with 18:2) and diacylglycerol (12.5%
with 16:0 vs. < 0.5% with 18:2) and diacylglycerol (12.5% with 16:0 vs.
0.5% with 18:2) and the amount of intracellular phospholipid doubled. The
morphological changes were completely reversed after 24 h with 1.0 mM 18:2.
We conclude from our results that, compared to 18:2, 16:0 is not
efficiently incorporated into triglycerides. 16:0 is incorporated into
cellular phospholipids in a greater proportion than 18:2, causing
accumulation of intracellular phospholipid and the precursors phosphatidic
acid and diacylglycerol. Different processing of 18:2 and 16:0 by Caco-2
cells resulted in profound differences in triglyceride synthesis and
lipoprotein composition and secretion.
ARTICLES
Palmitic acid and linoleic acid metabolism in Caco-2 cells: different triglyceride synthesis and lipoprotein secretion
Department of Medicine, Faculty of Medicine, Utrecht University, The Netherlands.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  K. G Jackson, E. J Wolstencroft, P. A Bateman, P. Yaqoob, and C. M Williams Greater enrichment of triacylglycerol-rich lipoproteins with apolipoproteins E and C-III after meals rich in saturated fatty acids than after meals rich in unsaturated fatty acids Am. J. Clinical Nutrition, January 1, 2005; 81(1): 25 - 34. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Dashti, Q. Feng, M. R. Freeman, M. Gandhi, and F. A. Franklin Trans Polyunsaturated Fatty Acids Have More Adverse Effects than Saturated Fatty Acids on the Concentration and Composition of Lipoproteins Secreted by Human Hepatoma HepG2 Cells J. Nutr., September 1, 2002; 132(9): 2651 - 2659. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. L. Black, H. M. Roche, and M. J. Gibney Chronic but Not Acute Treatment with Conjugated Linoleic Acid (CLA) Isomers (trans-10, cis-12 CLA and cis-9, trans-11 CLA) Affects Lipid Metabolism in Caco-2 Cells J. Nutr., August 1, 2002; 132(8): 2167 - 2173. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Chen, R. L Fitzgerald, and G. Schonfeld Hypobetalipoproteinemic Mice with a Targeted Apolipoprotein (Apo) B-27.6-specifying Mutation. IN VIVO EVIDENCE FOR AN IMPORTANT ROLE OF AMINO ACIDS 1254-1744 OF ApoB IN LIPID TRANSPORT AND METABOLISM OF THE ApoB-CONTAINING LIPOPROTEIN J. Biol. Chem., April 12, 2002; 277(16): 14135 - 14145. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Rong, S. Ramachandran, M. Penumetcha, N. Khan, and S. Parthasarathy Dietary oxidized fatty acids may enhance intestinal apolipoprotein A-I production J. Lipid Res., April 1, 2002; 43(4): 557 - 564. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Nayak, E. H. Harrison, and M. M. Hussain Retinyl ester secretion by intestinal cells: a specific and regulated process dependent on assembly and secretion of chylomicrons J. Lipid Res., February 1, 2001; 42(2): 272 - 280. [Abstract] [Full Text]
|
|
|
|
|
|
R. Abia, Y. M. Pacheco, J. S. Perona, E. Montero, F. J. G. Muriana, and V. Ruiz-Gutiérrez The Metabolic Availability of Dietary Triacylglycerols from Two High Oleic Oils during the Postprandial Period Does Not Depend on the Amount of Oleic Acid Ingested by Healthy Men J. Nutr., January 1, 2001; 131(1): 59 - 65. [Abstract] [Full Text]
|
|
|
|
|
|
M. M. J. van Greevenbroek, M. G. Robertus-Teunissen, D. W. Erkelens, and T. W. A. de Bruin Participation of the microsomal triglyceride transfer protein in lipoprotein assembly in Caco-2 cells: interaction with saturated and unsaturated dietary fatty acids J. Lipid Res., January 1, 1998; 39(1): 173 - 185. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. Luchoomun, Z. Zhou, A. Bakillah, H. Jamil, and M. M. Hussain Assembly and Secretion of VLDL in Nondifferentiated Caco-2 Cells Stably Transfected With Human Recombinant ApoB48 cDNA Arterioscler. Thromb. Vasc. Biol., November 1, 1997; 17(11): 2955 - 2963. [Abstract] [Full Text]
|
|
|
|
|
|
S.-Y. Ho, L. Delgado, and J. Storch Monoacylglycerol Metabolism in Human Intestinal Caco-2 Cells. EVIDENCE FOR METABOLIC COMPARTMENTATION AND HYDROLYSIS J. Biol. Chem., January 11, 2002; 277(3): 1816 - 1823. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |