HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Breuer, O. Search for Related Content PubMed PubMed Citation Articles by Breuer, O. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 36, 2275-2281, Copyright © 1995 by Lipid Research, Inc.

ARTICLES

Identification and quantitation of cholest-5-ene-3 beta,4 beta-diol in rat liver and human plasma

O Breuer
Karolinska Institutet, Department of Medical Laboratory Sciences and Technology, Huddinge University Hospital, Sweden.

Cholest-5-ene-3 beta,4 beta-diol (4 beta-hydroxycholesterol) was identified in human plasma and rat liver by gas-liquid chromatography- mass spectrometry. An assay based on isotope dilution-mass spectrometry with a deuterium-labeled internal standard was developed for quantitation of this compound. The concentration of cholest-5-ene-3 beta,4 beta-diol in plasma from healthy subjects was 36 +/- 4.3 ng/ml (mean +/- SD, n = 8). The concentration in rat liver was 0.62 +/- 0.19 microgram/g wet weight (mean +/- SD, n = 6). These levels are of the same order of magnitude as other common oxysterols.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				K. Bodin, U. Andersson, E. Rystedt, E. Ellis, M. Norlin, I. Pikuleva, G. Eggertsen, I. Bjorkhem, and U. Diczfalusy Metabolism of 4beta -Hydroxycholesterol in Humans J. Biol. Chem., August 23, 2002; 277(35): 31534 - 31540. [Abstract] [Full Text] [PDF]

				K. Bodin, L. Bretillon, Y. Aden, L. Bertilsson, U. Broome, C. Einarsson, and U. Diczfalusy Antiepileptic Drugs Increase Plasma Levels of 4beta -Hydroxycholesterolin Humans. EVIDENCE FOR INVOLVEMENT OF CYTOCHROME P450 3A4 J. Biol. Chem., October 12, 2001; 276(42): 38685 - 38689. [Abstract] [Full Text] [PDF]

				G. J. Schroepfer Jr. Oxysterols: Modulators of Cholesterol Metabolism and Other Processes Physiol Rev, January 1, 2000; 80(1): 361 - 554. [Abstract] [Full Text] [PDF]

				I. C. Gelissen, A. J. Brown, E. L. Mander, L. Kritharides, R. T. Dean, and W. Jessup Sterol Efflux Is Impaired from Macrophage Foam Cells Selectively Enriched with 7-Ketocholesterol J. Biol. Chem., July 26, 1996; 271(30): 17852 - 17860. [Abstract] [Full Text] [PDF]