| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Journal of Lipid Research, Vol 36, 2433-2443, Copyright © 1995 by Lipid Research, Inc.
SA Moore, E Hurt, E Yoder, H Sprecher and AA Spector
The purpose of this study was to determine whether the formation of
docosahexaenoic acid in human cells occurs through a pathway that involves
24-carbon n-3 fatty acid intermediates and retroconversion. Normal human
skin fibroblasts synthesized radiolabeled docosahexaenoic acid from
[1-(14)C]18:3n-3, [3-(14)C]22:5n-3, [3-(14)C]24:5n-3, and [3-
(14)C]24:6n-3. The amount of docosahexaenoate formed was reduced in
fibroblasts defective in peroxisomal biogenesis, by 90-100% in Zellweger's
syndrome and by 50-75% in infantile Refsum's disease. Fatty acid elongation
and desaturation were intact in these mutant cells. No decrease in
radiolabeled docosahexaenoic acid production occurred in mutant fibroblasts
defective in peroxisomal alpha-oxidation or mitochondrial beta-oxidation,
or in normal fibroblasts treated with methyl palmoxirate to inhibit
mitochondrial beta-oxidation. Therefore, the retroconversion step in
docosahexaenoic acid formation occurs through peroxisomal beta-oxidation in
normal human cells. These results demonstrate that the pathway for
docosahexaenoic acid synthesis in human cells involves 24-carbon
intermediates. The limited ability to synthesize docosahexaenoic acid may
underlie some of the pathology that occurs in genetic diseases involving
peroxisomal beta-oxidation.
ARTICLES
Docosahexaenoic acid synthesis in human skin fibroblasts involves peroxisomal retroconversion of tetracosahexaenoic acid
Department of Pathology, University of Iowa, Iowa City 52242, USA.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. C. DeMar Jr., C. DiMartino, A. W. Baca, W. Lefkowitz, and N. Salem Jr. Effect of dietary docosahexaenoic acid on biosynthesis of docosahexaenoic acid from alpha-linolenic acid in young rats J. Lipid Res., September 1, 2008; 49(9): 1963 - 1980. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Jia, C. L. Moulson, Z. Pei, J. H. Miner, and P. A. Watkins Fatty Acid Transport Protein 4 Is the Principal Very Long Chain Fatty Acyl-CoA Synthetase in Skin Fibroblasts J. Biol. Chem., July 13, 2007; 282(28): 20573 - 20583. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Morais, A. Knoll-Gellida, M. Andre, C. Barthe, and P. J. Babin Conserved expression of alternative splicing variants of peroxisomal acyl-CoA oxidase 1 in vertebrates and developmental and nutritional regulation in fish Physiol Genomics, February 12, 2007; 28(3): 239 - 252. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Huyghe, H. Schmalbruch, L. Hulshagen, P. V. Veldhoven, M. Baes, and D. Hartmann Peroxisomal Multifunctional Protein-2 Deficiency Causes Motor Deficits and Glial Lesions in the Adult Central Nervous System Am. J. Pathol., April 1, 2006; 168(4): 1321 - 1334. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ferdinandusse, S. Denis, G. Dacremont, and R. J. A. Wanders Studies on the metabolic fate of n-3 polyunsaturated fatty acids J. Lipid Res., October 1, 2003; 44(10): 1992 - 1997. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Watkins, P. R. Reifsnyder, H.-j. Pan, J. B. German, and E. H. Leiter Lipid metabolome-wide effects of the PPAR{gamma} agonist rosiglitazone J. Lipid Res., November 1, 2002; 43(11): 1809 - 1817. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ferdinandusse, S. Denis, P. A. W. Mooijer, Z. Zhang, J. K. Reddy, A. A. Spector, and R. J. A. Wanders Identification of the peroxisomal {beta}-oxidation enzymes involved in the biosynthesis of docosahexaenoic acid J. Lipid Res., December 1, 2001; 42(12): 1987 - 1995. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. E. Williard, S. D. Harmon, T. L. Kaduce, M. Preuss, S. A. Moore, M. E. C. Robbins, and A. A. Spector Docosahexaenoic acid synthesis from n-3 polyunsaturated fatty acids in differentiated rat brain astrocytes J. Lipid Res., September 1, 2001; 42(9): 1368 - 1376. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. E. Williard, J. O. Nwankwo, T. L. Kaduce, S. D. Harmon, M. Irons, H. W. Moser, G. V. Raymond, and A. A. Spector Identification of a fatty acid {{Delta}}6-desaturase deficiency in human skin fibroblasts J. Lipid Res., April 1, 2001; 42(4): 501 - 508. [Abstract] [Full Text]
|
|
|
|
 |
|
 R. Schultz, W. Yan, J. Toppari, A. Völkl, J.-A. Gustafsson, and M. Pelto-Huikko Expression of Peroxisome Proliferator-Activated Receptor {alpha} Messenger Ribonucleic Acid and Protein in Human and Rat Testis Endocrinology, July 1, 1999; 140(7): 2968 - 2975. [Abstract] [Full Text]
|
|
|
|
|
|
D. P. Gaposchkin and R. A. Zoeller Plasmalogen status influences docosahexaenoic acid levels in a macrophage cell line: insights using ether lipid-deficient variants J. Lipid Res., March 1, 1999; 40(3): 495 - 503. [Abstract] [Full Text]
|
|
|
|
|
|
N. Bernoud, L. Fenart, C. Bénistant, J. F. Pageaux, M. P. Dehouck, P. Molière, M. Lagarde, R. Cecchelli, and J. Lecerf Astrocytes are mainly responsible for the polyunsaturated fatty acid enrichment in blood–brain barrier endothelial cells in vitro J. Lipid Res., September 1, 1998; 39(9): 1816 - 1824. [Abstract] [Full Text]
|
|
|
|
|
|
D. E. Williard, T. L. Kaduce, S. D. Harmon, and A. A. Spector Conversion of eicosapentaenoic acid to chain-shortened omega–3 fatty acid metabolites by peroxisomal oxidation J. Lipid Res., May 1, 1998; 39(5): 978 - 986. [Abstract] [Full Text]
|
|
|
|
|
|
D. L. Luthria, B. S. Mohammed, and H. Sprecher Regulation of the Biosynthesis of 4,7,10,13,16,19-Docosahexaenoic Acid J. Biol. Chem., July 5, 1996; 271(27): 16020 - 16025. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-M. Su, A. B. Moser, H. W. Moser, and P. A. Watkins Peroxisomal Straight-chain Acyl-CoA Oxidase and D-bifunctional Protein Are Essential for the Retroconversion Step in Docosahexaenoic Acid Synthesis J. Biol. Chem., October 5, 2001; 276(41): 38115 - 38120. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |
