HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Liu, X. Q. Articles by Bagdade, J. D. Search for Related Content PubMed PubMed Citation Articles by Liu, X. Q. Articles by Bagdade, J. D. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 36, 2574-2579, Copyright © 1995 by Lipid Research, Inc.

ARTICLES

Neutral lipid mass transfer among lipoproteins in plasma from normolipidemic subjects is not an equimolar heteroexchange

XQ Liu and JD Bagdade
Section of Endocrinology and Metabolism, Rush Medical College, Chicago, IL 60612, USA.

To further characterize the cholesteryl ester transfer protein (CETP)- mediated distribution of neutral lipids that occurs among lipoproteins in plasma, the net mass transfer of core lipids between donor and acceptor lipoproteins in intact plasma was measured in ten healthy normolipidemic subjects. The rate of loss of cholesteryl ester (CE) from high density lipoprotein-3 (HDL3) (19.5 +/- 8.8 nmol/ml per h) was linear and increased significantly (P < 0.01) during the 6-h incubation. Approximately 50% of the CE transferred from HDL3 (118.7 +/- 54.3 nmol/ml) went to very low density lipoprotein (VLDL); the remainder was distributed to low density lipoprotein (LDL) (approximately 30%) and HDL2 (approximately 20%). The rate of loss of triglyceride (TG) from VLDL (14.5 +/- 6.6 nmol/ml per h) to the HDL subfractions and LDL also was linear and increased significantly with time (P < 0.01). About 50% of the TG mass lost from VLDL (85.2 +/- 38.4 nmol/ml) was transferred to LDL and the remainder was recovered in HDL2 (approximately 10%) and HDL3 (approximately 40%). As the number of nmoles of CE lost from HDL3 was almost three times greater than the nmoles of TG it acquired, these findings indicate that the exchange of core lipids in plasma that result from the interaction between CETP- VLDL-HDL3 is not equimolar. Even in the absence of VLDL, HDL3 continued to donate CE to LDL and HDL2 to almost the same degree as in intact plasma (plasma minus VLDL: 17.5 +/- 5.9 nmol/ml per h vs. intact plasma: 20.2 +/- 7.5 nmol/ml per h) without accepting any TG. Our findings demonstrate that independent pathways exist for the transfer of CE and TG among the plasma lipoproteins and, contrary to what is generally believed, a heteroexchange of TG for CE during cholesteryl ester transfer is not obligatory.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				V. M. Paromov and R. E. Morton Lipid Transfer Inhibitor Protein Defines the Participation of High Density Lipoprotein Subfractions in Lipid Transfer Reactions Mediated by Cholesterol Ester Transfer Protein (CETP) J. Biol. Chem., October 17, 2003; 278(42): 40859 - 40866. [Abstract] [Full Text] [PDF]

				R. E. Morton and D. J. Greene The surface cholesteryl ester content of donor and acceptor particles regulates CETP: a liposome-based approach to assess the substrate properties of lipoproteins J. Lipid Res., July 1, 2003; 44(7): 1364 - 1372. [Abstract] [Full Text] [PDF]

				A. P. Serdyuk and R. E. Morton Lipid Transfer Inhibitor Protein Defines the Participation of Lipoproteins in Lipid Transfer Reactions : CETP Has No Preference for Cholesteryl Esters in HDL Versus LDL Arterioscler. Thromb. Vasc. Biol., March 1, 1999; 19(3): 718 - 726. [Abstract] [Full Text] [PDF]

				B. H. Chung, F. Franklin, B. H. S. Cho, J. P. Segrest, K. Hart, and B. E. Darnell Potencies of Lipoproteins in Fasting and Postprandial Plasma to Accept Additional Cholesterol Molecules Released From Cell Membranes Arterioscler. Thromb. Vasc. Biol., August 1, 1998; 18(8): 1217 - 1230. [Abstract] [Full Text] [PDF]

				M. J. Liinamaa, M. L. Hannuksela, Y. A. Kesaniemi, and M. J. Savolainen Altered Transfer of Cholesteryl Esters and Phospholipids in Plasma From Alcohol Abusers Arterioscler. Thromb. Vasc. Biol., November 1, 1997; 17(11): 2940 - 2947. [Abstract] [Full Text]