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Journal of Lipid Research, Vol 36, 911-915, Copyright © 1995 by Lipid Research, Inc.

ARTICLES

Chemiluminescence detection of gangliosides by thin-layer chromatography

SL Arnsmeier and AS Paller
Department of Pediatrics, Children's Memorial Institute for Education and Research, Northwestern University Medical School, Chicago, IL 60614, USA.

We describe a method for the detection of gangliosides on thin-layer chromatography plates using enhanced chemiluminescence. In contrast to previously published colorimetric techniques, the use of chemiluminescence of detect antibody binding to gangliosides has increased sensitivity and simplicity. In addition, the use of chemiluminescence lacks the hazards of radioactivity, and produces a permanent record.
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