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Journal of Lipid Research, Vol 36, 975-985, Copyright © 1995 by Lipid Research, Inc.
P Pussinen, M Jauhianinen, J Metso, J Tyynela and C Ehnholm
Phospholipid transfer protein (PLTP) from pig plasma was purified to
homogeneity using ultracentrifugation and a combination of hydrophobic- ,
heparin-Sepharose-, and anti-pig-PLTP-affinity chromatography techniques.
The molecular weight of PLTP is 78,000 as estimated by SDS- PAGE and by gel
filtration. The effect of pig plasma PLTP on the particle size distribution
of either human or pig high density lipoprotein (HDL) was studied by
incubating HDL with PLTP. Incubation of human HDL3 or pig HDL with the
highly purified preparations of PLTP induced a conversion of the
homogeneous HDL into two main populations of particles. The conversion
products were isolated by ultracentrifugation at density 1.21 g/ml. The
size changes were evident as analyzed by native gradient gel
electrophoresis and by high resolution gel filtration. The diameters of the
large and small particles formed were 10.8 nm and 7.6-7.9 nm, respectively.
In addition, pig HDL conversion products included a third population of
particles with a diameter of 11.5 nm. The degree of conversion was
dependent on time and PLTP activity. Neither cholesteryl ester transfer
protein nor lecithin:cholesterol acyltransferase activity could be detected
in the PLTP preparations. The present study demonstrates that purified pig
PLTP can act as a conversion factor: it has the ability to convert HDL into
populations of large and small particles. The release of apoA-I is an
essential part of the conversion process.
ARTICLES
Pig plasma phospholipid transfer protein facilitates HDL interconversion
National Public Health Institute, Department of Biochemistry, Helsinki, Finland.
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