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Journal of Lipid Research, Vol 36, 1236-1246, Copyright © 1995 by Lipid Research, Inc.
X Fang, M VanRollins, TL Kaduce and AA Spector
Epoxyeicosatrienoic acids (EETs) are eicosanoids synthesized from
arachidonic acid by the cytochrome P450 eposygenase pathway. The present
studies demonstrate that 8,9-, 11,12-, and 14,15-EET are rapidly taken up
by porcine aortic smooth muscle cells. About half of the uptake is
incorporated into phospholipids, and saponification indicates that most of
this remains in the form of EET. The EETs also are converted to the
corresponding dihydroxyeicosatrienoic acids (DHETs) and during prolonged
incubations, additional metabolites that do not retain the EET carboxyl
group are formed. Most of these products are released into the medium.
However, some DHET and metabolites less polar than EET are incorporated
into the phospholipids, and a small amount of unesterified EET is also
present in the cells. The incorporation of 14,15-EET and its conversion to
DHET did not approach saturation until the concentration exceeded 10-20
microM, indicating that vascular smooth muscle has a large capacity to
utilize this EET. These findings suggest that certain vasoactive effects of
EETs may be due to their incorporation by smooth muscle cells. Furthermore,
through conversion to DHET and other oxidized metabolites, smooth muscle
apparently has the capacity to inactivate EETs that are either formed in or
penetrate into the vascular wall.
ARTICLES
Epoxyeicosatrienoic acid metabolism in arterial smooth muscle cells
Department of Biochemistry, University of Iowa College of Medicine, Iowa City 52242, USA.
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