HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Dietrich, A. Articles by Kurz, G. Search for Related Content PubMed PubMed Citation Articles by Dietrich, A. Articles by Kurz, G. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 36, 1745-1755, Copyright © 1995 by Lipid Research, Inc.

ARTICLES

Functional significance of interaction of H-FABP with sulfated and nonsulfated taurine-conjugated bile salts in rat liver

A Dietrich, W Dieminger, K Fuchte, GH Stoll, E Schlitz, W Gerok and G Kurz
Institut fur Organische Chemie und Biochemie, Universitat Freiburg, Germany.

To identify the cytosolic proteins of rat hepatocytes involved in transcellular transport of sulfated and taurine-conjugated bile salts in comparison with only taurine-conjugated bile salts, photoaffinity labeling studies were performed with [3H]-7,7-ASLCT (7,7-azi-3 alpha- sulfatolithocholyl[2'-3H(N)]taurine), [3H]-7,7-ACT ((7,7-azi-3 alpha,12 alpha-dihydroxy-5 beta-cholan-24-oyl)- 2'-[2'- 3H(N)]aminoethanesulfonate), and [3H]-7,7-ALCT (7,7-azilithocholyl[2'- 3H(N)]taurine) using isolated hepatocytes and intact liver tissue. Photoaffinity labeling of isolated hepatocytes with [3H]-7,7-ASLCT on the one hand and with [3H]-7,7-ACT and [3H]-7,7-ALCT on the other resulted in a different labeling pattern of cytosolic polypeptides, without a relevant incorporation of radioactivity into subunits of glutathione transferases. This suggests that glutathione transferases play no role in the transport of dianionic or of monoanionic bile salts. With [3H]-7,7-ACT and [3H]-7,7-ALCT a moderate incorporation of radioactivity was found in polypeptides with apparent M(r)s of 33,000, 38,000, and 54,000, whereas with [3H]-7,7-ASLCT, a polypeptide with an apparent M(r) of 14,000, identified as H-FABP, was markedly and almost exclusively labeled. Photoaffinity labeling of specimens of intact liver tissue resulted in a labeling pattern of cytosolic polypeptides comparable to that obtained from photolabeled isolated hepatocytes. All results suggest that transcellular transport of dianionic sulfated as well as taurine-conjugated bile salts and of monoanionic taurine- conjugated bile salts follows different pathways. In intracellular transport of taurine-conjugated bile salts, several cytosolic polypeptides may have a function, whereas, in transport of taurine- conjugated 3 alpha-sulfato bile salts, only H-FABP appears to be involved.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				G. G. Martin, B. P. Atshaves, A. L. McIntosh, J. T. Mackie, A. B. Kier, and F. Schroeder Liver fatty acid binding protein gene ablation potentiates hepatic cholesterol accumulation in cholesterol-fed female mice Am J Physiol Gastrointest Liver Physiol, January 1, 2006; 290(1): G36 - G48. [Abstract] [Full Text] [PDF]

				R. M. Hagan, J. Worner-Gibbs, and D. C. Wilton Tryptophan Insertion Mutagenesis of Liver Fatty Acid-binding Protein: L28W MUTANT PROVIDES IMPORTANT INSIGHTS INTO LIGAND BINDING AND PHYSIOLOGICAL FUNCTION J. Biol. Chem., January 21, 2005; 280(3): 1782 - 1789. [Abstract] [Full Text] [PDF]

				E. P. Newberry, Y. Xie, S. Kennedy, X. Han, K. K. Buhman, J. Luo, R. W. Gross, and N. O. Davidson Decreased Hepatic Triglyceride Accumulation and Altered Fatty Acid Uptake in Mice with Deletion of the Liver Fatty Acid-binding Protein Gene J. Biol. Chem., December 19, 2003; 278(51): 51664 - 51672. [Abstract] [Full Text] [PDF]