HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Watanabe, K. Articles by Mizuta, M. Search for Related Content PubMed PubMed Citation Articles by Watanabe, K. Articles by Mizuta, M. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 36, 1848-1855, Copyright © 1995 by Lipid Research, Inc.

ARTICLES

Fluorometric detection of glycosphingolipids on thin-layer chromatographic plates

K Watanabe and M Mizuta
Division of Biochemistry, Shigei Medical Research Institute, Okayama, Japan.

A microdetection system for glycosphingolipid analysis has been developed using 5-hydroxy-1-tetralone as the fluorescent labeling reagent. The reagents in H2SO4 permit the fluorometric detection of acidic and neutral glycosphingolipids both in test tube and on thin- layer chromatographic plates. Glycosphingolipids can be detected at concentrations as low as 5 pmol on the thin-layer chromatographic plate. The method is a rapid and simple, and feasible for determination of glycosphingolipid from small amounts of biological samples.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				A. Puri, P. Hug, K. Jernigan, J. Barchi, H.-Y. Kim, J. Hamilton, J. Wiels, G. J. Murray, R. O. Brady, and R. Blumenthal The neutral glycosphingolipid globotriaosylceramide promotes fusion mediated by a CD4-dependent CXCR4-utilizing HIV type 1 envelope glycoprotein PNAS, November 24, 1998; 95(24): 14435 - 14440. [Abstract] [Full Text] [PDF]