| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Journal of Lipid Research, Vol 37, 210-220, Copyright © 1996 by Lipid Research, Inc.
X Li, F Catalina, SM Grundy and S Patel
We have developed a procedure to measure the rates of apolipoprotein (apoB)
and triglyceride secretion from the liver of an individual mouse. Using the
well-characterized method of Triton WR-1339 injection to block peripheral
removal of newly secreted VLDL, the rate of triglyceride accumulation is
monitored and at the end of the experimental period, blood is extracted for
quantitative VLDL preparation. ApoB species in isolated VLDL are analyzed
by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and the mass
of the apoB-48 and apoB-100 species are estimated by Coomassie staining and
laser densitometric scanning, using known quantities of LDL-apoB-100 as
standards. This methodology was applied to measure the rate of apoB
secretion in male and female FVB/N mice and we found that the molar ratio
of newly secreted ApoB-48 to B-100 is 4.6 in the male, and 3.8 in the
female. Measurements of the steady state apoB levels indicate that
liver-derived apoB-48 is cleared from the circulation 7.1 times faster than
B-100 in the male and 4.7 times faster in the female mouse. VLDL apoB-48
fractional turnover is approximately 1800 pools per day in both the male
and female mouse (1814 +/- 139 vs. 1831 +/- 365 respectively, P = 0.92).
ApoB-100 fractional turnover rates are much slower and show a statistically
significant difference between males and females (255 +/- 19 pools per day
vs. 386 +/- 65 pools per day, respectively, P = 0.006). This procedure
provides for quantification of secretory rates of these apo proteins in
vivo, and may be useful for studying the effects of genetic manipulation on
the simultaneous secretion of apoB- 48- and apoB-100-containing VLDL,
afforded by the panoply of transgenic mouse models now available for study,
as well as for effects of diet and drug therapy.
ARTICLES
Method to measure apolipoprotein B-48 and B-100 secretion rates in an individual mouse: evidence for a very rapid turnover of VLDL and preferential removal of B-48- relative to B-100-containing lipoproteins
Center for Human Nutrition, UT Southwestern Medical Center, Dallas, TX 75235-9052, USA.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  L. Shelly, L. Royer, T. Sand, H. Jensen, and Y. Luo Phospholipid transfer protein deficiency ameliorates diet-induced hypercholesterolemia and inflammation in mice J. Lipid Res., April 1, 2008; 49(4): 773 - 781. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Li, V. Savransky, A. Nanayakkara, P. L. Smith, C. P. O'Donnell, and V. Y. Polotsky Hyperlipidemia and lipid peroxidation are dependent on the severity of chronic intermittent hypoxia J Appl Physiol, February 1, 2007; 102(2): 557 - 563. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Duivenvoorden, P. J Voshol, P. C. Rensen, W. van Duyvenvoorde, J. A Romijn, J. J Emeis, L. M Havekes, and W. F Nieuwenhuizen Dietary sphingolipids lower plasma cholesterol and triacylglycerol and prevent liver steatosis in APOE*3Leiden mice. Am. J. Clinical Nutrition, August 1, 2006; 84(2): 312 - 321. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Linden, L. William-Olsson, A. Ahnmark, K. Ekroos, C. Hallberg, H. P. Sjogren, B. Becker, L. Svensson, J. C. Clapham, J. Oscarsson, et al. Liver-directed overexpression of mitochondrial glycerol-3-phosphate acyltransferase results in hepatic steatosis, increased triacylglycerol secretion and reduced fatty acid oxidation FASEB J, March 1, 2006; 20(3): 434 - 443. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Li, L. N. Thorne, N. M. Punjabi, C.-K. Sun, A. R. Schwartz, P. L. Smith, R. L. Marino, A. Rodriguez, W. C. Hubbard, C. P. O'Donnell, et al. Intermittent Hypoxia Induces Hyperlipidemia in Lean Mice Circ. Res., September 30, 2005; 97(7): 698 - 706. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Millar, D. A. Cromley, M. G. McCoy, D. J. Rader, and J. T. Billheimer Determining hepatic triglyceride production in mice: comparison of poloxamer 407 with Triton WR-1339 J. Lipid Res., September 1, 2005; 46(9): 2023 - 2028. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Duivenvoorden, B. Teusink, P. C. N. Rensen, F. Kuipers, J. A. Romijn, L. M. Havekes, and P. J. Voshol Acute inhibition of hepatic {beta}-oxidation in APOE*3Leiden mice does not affect hepatic VLDL secretion or insulin sensitivity J. Lipid Res., May 1, 2005; 46(5): 988 - 993. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Olsson, M. Bohlooly-Y, S. M. Fitzgerald, F. Frick, A. Ljungberg, B. Ahren, J. Tornell, G. Bergstrom, and J. Oscarsson Bovine Growth Hormone Transgenic Mice Are Resistant to Diet-Induced Obesity but Develop Hyperphagia, Dyslipidemia, and Diabetes on a High-Fat Diet Endocrinology, February 1, 2005; 146(2): 920 - 930. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Ge, G. Yang, X. Yu, T. Pourbahrami, and C. Li Oligomerization state-dependent hyperlipidemic effect of angiopoietin-like protein 4 J. Lipid Res., November 1, 2004; 45(11): 2071 - 2079. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Fu, D. Mukhopadhyay, N. O. Davidson, and J. Borensztajn The Peroxisome Proliferator-activated Receptor {alpha} (PPAR{alpha}) Agonist Ciprofibrate Inhibits Apolipoprotein B mRNA Editing in Low Density Lipoprotein Receptor-deficient Mice: EFFECTS ON PLASMA LIPOPROTEINS AND THE DEVELOPMENT OF ATHEROSCLEROTIC LESIONS J. Biol. Chem., July 2, 2004; 279(27): 28662 - 28669. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Alfadda, R. A. DosSantos, Z. Stepanyan, H. Marrif, and J. E. Silva Mice with deletion of the mitochondrial glycerol-3-phosphate dehydrogenase gene exhibit a thrifty phenotype: effect of gender Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2004; 287(1): R147 - R156. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Stiles, Y. Wang, A. Stahl, S. Bassilian, W. P. Lee, Y.-J. Kim, R. Sherwin, S. Devaskar, R. Lesche, M. A. Magnuson, et al. Liver-specific deletion of negative regulator Pten results in fatty liver and insulin hypersensitivity PNAS, February 17, 2004; 101(7): 2082 - 2087. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. R. Webb, M. C. de Beer, F. C. de Beer, and D. R. van der Westhuyzen ApoB-containing lipoproteins in apoE-deficient mice are not metabolized by the class B scavenger receptor BI J. Lipid Res., February 1, 2004; 45(2): 272 - 280. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Zoltowska, E. Ziv, E. Delvin, M. Lambert, E. Seidman, and E. Levy Both Insulin Resistance and Diabetes in Psammomys obesus Upregulate the Hepatic Machinery Involved in Intracellular VLDL Assembly Arterioscler. Thromb. Vasc. Biol., January 1, 2004; 24(1): 118 - 123. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. H. Wiegman, R. H.J. Bandsma, M. Ouwens, F. H. van der Sluijs, R. Havinga, T. Boer, D.-J. Reijngoud, J. A. Romijn, and F. Kuipers Hepatic VLDL Production in ob/ob Mice Is Not Stimulated by Massive De Novo Lipogenesis but Is Less Sensitive to the Suppressive Effects of Insulin Diabetes, May 1, 2003; 52(5): 1081 - 1089. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Amigo, S. Zanlungo, J. F. Miquel, J. M. Glick, H. Hyogo, D. E. Cohen, A. Rigotti, and F. Nervi Hepatic overexpression of sterol carrier protein-2 inhibits VLDL production and reciprocally enhances biliary lipid secretion J. Lipid Res., February 1, 2003; 44(2): 399 - 407. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. E. Hammond, P. A. Gallagher, S. Wang, S. Hiller, K. D. Kluckman, E. L. Posey-Marcos, N. Maeda, and R. A. Coleman Mitochondrial Glycerol-3-Phosphate Acyltransferase-Deficient Mice Have Reduced Weight and Liver Triacylglycerol Content and Altered Glycerolipid Fatty Acid Composition Mol. Cell. Biol., December 1, 2002; 22(23): 8204 - 8214. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Frick, D. Linden, C. Ameen, S. Eden, A. Mode, and J. Oscarsson Interaction between growth hormone and insulin in the regulation of lipoprotein metabolism in the rat Am J Physiol Endocrinol Metab, November 1, 2002; 283(5): E1023 - E1031. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Hinsdale, P. M. Sullivan, H. Mezdour, and N. Maeda ApoB-48 and apoB-100 differentially influence the expression of type-III hyperlipoproteinemia in APOE*2 mice J. Lipid Res., September 1, 2002; 43(9): 1520 - 1528. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Millar, C. Maugeais, I. V. Fuki, and D. J. Rader Normal Production Rate of Apolipoprotein B in LDL Receptor-Deficient Mice Arterioscler. Thromb. Vasc. Biol., June 1, 2002; 22(6): 989 - 994. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Vasandani, A. I. Kafrouni, A. Caronna, Y. Bashmakov, M. Gotthardt, J. D. Horton, and D. K. Spady Upregulation of hepatic LDL transport by n-3 fatty acids in LDL receptor knockout mice J. Lipid Res., May 1, 2002; 43(5): 772 - 784. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Galman, B. Angelin, and M. Rudling Prolonged Stimulation of the Adrenals by Corticotropin Suppresses Hepatic Low-Density Lipoprotein and High-Density Lipoprotein Receptors and Increases Plasma Cholesterol Endocrinology, May 1, 2002; 143(5): 1809 - 1816. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Frick, M. Bohlooly-Y, D. Linden, B. Olsson, J. Tornell, S. Eden, and J. Oscarsson Long-term growth hormone excess induces marked alterations in lipoprotein metabolism in mice Am J Physiol Endocrinol Metab, December 1, 2001; 281(6): E1230 - E1239. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Linden, M. Alsterholm, H. Wennbo, and J. Oscarsson PPAR{alpha} deficiency increases secretion and serum levels of apolipoprotein B-containing lipoproteins J. Lipid Res., November 1, 2001; 42(11): 1831 - 1840. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. H.J. Bandsma, C. H. Wiegman, A. W. Herling, H.-J. Burger, A. ter Harmsel, A. J. Meijer, J. A. Romijn, D.-J. Reijngoud, and F. Kuipers Acute Inhibition of Glucose-6-Phosphate Translocator Activity Leads to Increased De Novo Lipogenesis and Development of Hepatic Steatosis Without Affecting VLDL Production in Rats Diabetes, November 1, 2001; 50(11): 2591 - 2597. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. Mensenkamp, B. Teusink, J. F.W. Baller, H. Wolters, R. Havinga, K. W. van Dijk, L. M. Havekes, and F. Kuipers Mice Expressing Only the Mutant APOE3Leiden Gene Show Impaired VLDL Secretion Arterioscler. Thromb. Vasc. Biol., August 1, 2001; 21(8): 1366 - 1372. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Asset, E. Bauge, J. C. Fruchart, and J. Dallongeville Lack of Triglyceride-Lowering Properties of Fish Oil in Apolipoprotein E-Deficient Mice Arterioscler. Thromb. Vasc. Biol., March 1, 2001; 21(3): 401 - 406. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Maugeais, U. J. F. Tietge, K. Tsukamoto, J. M. Glick, and D. J. Rader Hepatic apolipoprotein E expression promotes very low density lipoprotein-apolipoprotein B production in vivo in mice J. Lipid Res., October 1, 2000; 41(10): 1673 - 1679. [Abstract] [Full Text]
|
|
|
|
|
|
A. R Mensenkamp, M. C Jong, H. van Goor, M. J. A. van Luyn, V. Bloks, R. Havinga, P. J. Voshol, M. H. Hofker, K. W. van Dijk, L. M. Havekes, et al. Apolipoprotein E Participates in the Regulation of Very Low Density Lipoprotein-Triglyceride Secretion by the Liver J. Biol. Chem., December 10, 1999; 274(50): 35711 - 35718. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Wu, Y. H. Zhu, and S. B. Patel Cyclosporin-induced dyslipoproteinemia is associated with selective activation of SREBP-2 Am J Physiol Endocrinol Metab, December 1, 1999; 277(6): E1087 - E1094. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Asset, E. Baugé, R. L. Wolff, J. C. Fruchart, and J. Dallongeville Pinus pinaster Oil Affects Lipoprotein Metabolism in Apolipoprotein E-Deficient Mice J. Nutr., November 1, 1999; 129(11): 1972 - 1978. [Abstract] [Full Text]
|
|
|
|
|
|
B. H.-J. Chang, W. Liao, L. Li, M. Nakamuta, D. Mack, and L. Chan Liver-specific Inactivation of the Abetalipoproteinemia Gene Completely Abrogates Very Low Density Lipoprotein/Low Density Lipoprotein Production in a Viable Conditional Knockout Mouse J. Biol. Chem., March 5, 1999; 274(10): 6051 - 6055. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Veniant, E. Kim, S. McCormick, J. Boren, L. B. Nielsen, M. Raabe, and S. G. Young Insights into Apolipoprotein B Biology from Transgenic and Gene-Targeted Mice J. Nutr., February 1, 1999; 129(2): 451 - 451. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Kim, P. Ambroziak, M. M. Veniant, R. L. Hamilton, and S. G. Young A Gene-targeted Mouse Model for Familial Hypobetalipoproteinemia. LOW LEVELS OF APOLIPOPROTEIN B mRNA IN ASSOCIATION WITH A NONSENSE MUTATION IN EXON 26 OF THE APOLIPOPROTEIN B GENE J. Biol. Chem., December 18, 1998; 273(51): 33977 - 33984. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Chen, R. L. Fitzgerald, M. R. Averna, and G. Schonfeld A Targeted Apolipoprotein B-38.9-producing Mutation Causes Fatty Livers in Mice Due to the Reduced Ability of Apolipoprotein B-38.9 to Transport Triglycerides J. Biol. Chem., October 13, 2000; 275(42): 32807 - 32815. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. K. Spady, M. N. Willard, and R. S. Meidell Role of Acyl-Coenzyme A:Cholesterol Acyltransferase-1 in the Control of Hepatic Very Low Density Lipoprotein Secretion and Low Density Lipoprotein Receptor Expression in the Mouse and Hamster J. Biol. Chem., August 25, 2000; 275(35): 27005 - 27012. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |
