| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Journal of Lipid Research, Vol 37, 45-53, Copyright © 1996 by Lipid Research, Inc.
H Itabe, H Yamamoto, T Imanaka, K Shimamura, H Uchiyama, J Kimura, T Sanaka, Y Hata and T Takano
We have established a new method capable of measuring the very low
concentrations of oxidized low density lipoprotein (OxLDL). In our previous
study, we obtained a novel murine monoclonal antibody against oxidized
lipoproteins (Itabe, H. et al. 1994. J. Biol. Chem. 269: 15274- 15279). The
epitope of this antibody resides in oxidized products of
phosphatidylcholine that can form complexes with polypeptides, including
apolipoprotein B. When the monoclonal antibody was precoated onto
microtiter wells prior to carrying out a sandwich ELISA using an anti-human
apolipoprotein B antibody, it was possible to detect 0.5 ng protein of
copper-induced OxLDL. The detection of OxLDL was dependent on the presence
of monoclonal antibody and was blocked by oxidized phosphatidylcholine
(OxPC). Under the same sandwich ELISA condition, native LDL showed a
dose-dependent increase of absorbance that was inhibited by complex of OxPC
with BSA. These results suggest the possible occurrence of oxidative
modification of human plasma LDL, which is recognized by the antibody
against OxPC. The level of LDL oxidation of normal human subjects was found
to be 0.52 +/- 0.35 units per 5 mu g protein of LDL, where one unit was
defined as the reactivity corresponding to 1 ng of copper-induced OxLDL by
this assay. Furthermore, we found that the LDL oxidation level in patients
who had been receiving hemodialysis treatment was increased more than
eightfold over that of normal subjects. We suggest that LDL in human plasma
is oxidatively modified under certain conditions and this method for
measurement of OxLDL could be used to study the relationship between in
vivo oxidation reaction and various pathological conditions.
ARTICLES
Sensitive detection of oxidatively modified low density lipoprotein using a monoclonal antibody
Department of Microbiology and Molecular Pathology, Teikyo University, Kanagawa, Japan.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  P. Castilla, A. Davalos, J. L. Teruel, F. Cerrato, M. Fernandez-Lucas, J. L. Merino, C. C. Sanchez-Martin, J. Ortuno, and M. A Lasuncion Comparative effects of dietary supplementation with red grape juice and vitamin E on production of superoxide by circulating neutrophil NADPH oxidase in hemodialysis patients Am. J. Clinical Nutrition, April 1, 2008; 87(4): 1053 - 1061. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Bro, C. J. Binder, J. L. Witztum, K. Olgaard, and L. B. Nielsen Inhibition of the Renin-Angiotensin System Abolishes the Proatherogenic Effect of Uremia in Apolipoprotein E-Deficient Mice Arterioscler. Thromb. Vasc. Biol., May 1, 2007; 27(5): 1080 - 1086. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. O. Apostolov, S. V. Shah, E. Ok, and A. G. Basnakian Carbamylated Low-Density Lipoprotein Induces Monocyte Adhesion to Endothelial Cells Through Intercellular Adhesion Molecule-1 and Vascular Cell Adhesion Molecule-1 Arterioscler. Thromb. Vasc. Biol., April 1, 2007; 27(4): 826 - 832. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Sosa, E. M. Balk, J. Lau, O. Liangos, V. S. Balakrishnan, N. E. Madias, B. J. G. Pereira, and B. L. Jaber A systematic review of the effect of the Excebrane dialyser on biomarkers of lipid peroxidation Nephrol. Dial. Transplant., October 1, 2006; 21(10): 2825 - 2833. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. G. Tsouli, D. N. Kiortsis, E. S. Lourida, V. Xydis, L. D. Tsironis, M. I. Argyropoulou, M. Elisaf, and A. D. Tselepis Autoantibody titers against OxLDL are correlated with Achilles tendon thickness in patients with familial hypercholesterolemia J. Lipid Res., October 1, 2006; 47(10): 2208 - 2214. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Tsimikas, J. T. Willerson, and P. M. Ridker C-reactive protein and other emerging blood biomarkers to optimize risk stratification of vulnerable patients. J. Am. Coll. Cardiol., April 18, 2006; 47(8 Suppl): C19 - C31. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Naruko, M. Ueda, S. Ehara, A. Itoh, K. Haze, N. Shirai, Y. Ikura, M. Ohsawa, H. Itabe, Y. Kobayashi, et al. Persistent High Levels of Plasma Oxidized Low-Density Lipoprotein After Acute Myocardial Infarction Predict Stent Restenosis Arterioscler. Thromb. Vasc. Biol., April 1, 2006; 26(4): 877 - 883. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Masuda, H. Itabe, M. Odaki, K. Hama, Y. Fujimoto, M. Mori, N. Sasabe, J. Aoki, H. Arai, and T. Takano ADRP/adipophilin is degraded through the proteasome-dependent pathway during regression of lipid-storing cells J. Lipid Res., January 1, 2006; 47(1): 87 - 98. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. O. Apostolov, S. V. Shah, E. Ok, and A. G. Basnakian Quantification of Carbamylated LDL in Human Sera by a New Sandwich ELISA Clin. Chem., April 1, 2005; 51(4): 719 - 728. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Passauer, F. Pistrosch, E. Bussemaker, G. Lassig, K. Herbrig, and P. Gross Reduced Agonist-Induced Endothelium-Dependent Vasodilation in Uremia Is Attributable to an Impairment of Vascular Nitric Oxide J. Am. Soc. Nephrol., April 1, 2005; 16(4): 959 - 965. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. L. Milne, J. R. Seal, C. M. Havrilla, M. Wijtmans, and N. A. Porter Identification and analysis of products formed from phospholipids in the free radical oxidation of human low density lipoproteins J. Lipid Res., February 1, 2005; 46(2): 307 - 319. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Virella, M. B. Derrick, V. Pate, C. Chassereau, S. R. Thorpe, and M. F. Lopes-Virella Development of Capture Assays for Different Modifications of Human Low-Density Lipoprotein Clin. Vaccine Immunol., January 1, 2005; 12(1): 68 - 75. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-L. Liu, K. Ylitalo, R. Salonen, J. T. Salonen, and M.-R. Taskinen Circulating Oxidized Low-Density Lipoprotein and Its Association With Carotid Intima-Media Thickness in Asymptomatic Members of Familial Combined Hyperlipidemia Families Arterioscler. Thromb. Vasc. Biol., August 1, 2004; 24(8): 1492 - 1497. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Matsumoto, H. Takashima, N. Ohira, Y. Tarutani, Y. Yasuda, T. Yamane, S. Matsuo, and M. Horie Plasma level of oxidized low-density lipoprotein is an independent determinant of coronary macrovasomotor and microvasomotor responses induced by bradykinin J. Am. Coll. Cardiol., July 21, 2004; 44(2): 451 - 457. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Itabe, M. Mori, Y. Fujimoto, Y. Higashi, and T. Takano Minimally Modified LDL Is an Oxidized LDL Enriched with Oxidized Phosphatidylcholines J. Biochem., September 1, 2003; 134(3): 459 - 465. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M Uno, K T Kitazato, K Nishi, H Itabe, and S Nagahiro Raised plasma oxidised LDL in acute cerebral infarction J. Neurol. Neurosurg. Psychiatry, March 1, 2003; 74(3): 312 - 316. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Nishi, H. Itabe, M. Uno, K. T. Kitazato, H. Horiguchi, K. Shinno, and S. Nagahiro Oxidized LDL in Carotid Plaques and Plasma Associates With Plaque Instability Arterioscler. Thromb. Vasc. Biol., October 1, 2002; 22(10): 1649 - 1654. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Tanaga, H. Bujo, M. Inoue, K. Mikami, K. Kotani, K. Takahashi, T. Kanno, and Y. Saito Increased Circulating Malondialdehyde-Modified LDL Levels in Patients With Coronary Artery Diseases and Their Association With Peak Sizes of LDL Particles Arterioscler. Thromb. Vasc. Biol., April 1, 2002; 22(4): 662 - 666. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Tsutsui, T. Tsutamoto, A. Wada, K. Maeda, N. Mabuchi, M. Hayashi, M. Ohnishi, and M. Kinoshita Plasma oxidized low-density lipoprotein as a prognostic predictor in patients with chronic congestive heart failure J. Am. Coll. Cardiol., March 20, 2002; 39(6): 957 - 962. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Mori, H. Itabe, Y. Higashi, Y. Fujimoto, M. Shiomi, M. Yoshizumi, Y. Ouchi, and T. Takano Foam cell formation containing lipid droplets enriched with free cholesterol by hyperlipidemic serum J. Lipid Res., November 1, 2001; 42(11): 1771 - 1781. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Tsutamoto, A. Wada, T. Matsumoto, K. Maeda, N. Mabuchi, M. Hayashi, T. Tsutsui, M. Ohnishi, M. Sawaki, M. Fujii, et al. Relationship between tumor necrosis factor-alpha production and oxidative stress in the failing hearts of patients with dilated cardiomyopathy J. Am. Coll. Cardiol., June 15, 2001; 37(8): 2086 - 2092. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Tsimikas and J. L. Witztum Measuring Circulating Oxidized Low-Density Lipoprotein to Evaluate Coronary Risk Circulation, April 17, 2001; 103(15): 1930 - 1932. [Full Text] [PDF]
|
|
|
|
|
|
S. Ehara, M. Ueda, T. Naruko, K. Haze, A. Itoh, M. Otsuka, R. Komatsu, T. Matsuo, H. Itabe, T. Takano, et al. Elevated Levels of Oxidized Low Density Lipoprotein Show a Positive Relationship With the Severity of Acute Coronary Syndromes Circulation, April 17, 2001; 103(15): 1955 - 1960. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P A Gordon, J George, M A Khamashta, D Harats, G Hughes, and Y Shoenfeld Atherosclerosis and autoimmunity Lupus, April 1, 2001; 10(4): 249 - 252. [Abstract] [PDF]
|
|
|
|
 |
|
 B. Halvorsen, A. C. Staff, T. Henriksen, T. Sawamura, and T. Ranheim 8-iso-Prostaglandin F2{{alpha}} Increases Expression of LOX-1 in JAR Cells Hypertension, April 1, 2001; 37(4): 1184 - 1190. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I S Young and J V Woodside Antioxidants in health and disease J. Clin. Pathol., March 1, 2001; 54(3): 176 - 186. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-i. Toshima, A. Hasegawa, M. Kurabayashi, H. Itabe, T. Takano, J. Sugano, K. Shimamura, J. Kimura, I. Michishita, T. Suzuki, et al. Circulating Oxidized Low Density Lipoprotein Levels : A Biochemical Risk Marker for Coronary Heart Disease Arterioscler. Thromb. Vasc. Biol., October 1, 2000; 20(10): 2243 - 2247. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. PASSAUER, E. BÜSSEMAKER, U. RANGE, M. PLUG, and P. GROSS Evidence In Vivo Showing Increase of Baseline Nitric Oxide Generation and Impairment of Endothelium-Dependent Vasodilation in Normotensive Patients on Chronic Hemodialysis J. Am. Soc. Nephrol., September 1, 2000; 11(9): 1726 - 1734. [Abstract] [Full Text]
|
|
|
|
|
|
H. Miyazaki, H. Matsuoka, H. Itabe, M. Usui, S. Ueda, S. Okuda, and T. Imaizumi Hemodialysis Impairs Endothelial Function via Oxidative Stress : Effects of Vitamin E-Coated Dialyzer Circulation, March 7, 2000; 101(9): 1002 - 1006. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Fukumoto, T. Shoji, M. Emoto, T. Kawagishi, Y. Okuno, and Y. Nishizawa Antibodies Against Oxidized LDL and Carotid Artery Intima-Media Thickness in a Healthy Population Arterioscler. Thromb. Vasc. Biol., March 1, 2000; 20(3): 703 - 707. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Deguchi, J. A. Fernandez, T. M. Hackeng, C. L. Banka, and J. H. Griffin Cardiolipin is a normal component of human plasma lipoproteins PNAS, February 15, 2000; 97(4): 1743 - 1748. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. ANDO, T. SANAKA, and H. NIHEI Eicosapentanoic Acid Reduces Plasma Levels of Remnant Lipoproteins and Prevents in Vivo Peroxidation of LDL in Dialysis Patients J. Am. Soc. Nephrol., October 1, 1999; 10(10): 2177 - 2184. [Abstract] [Full Text]
|
|
|
|
|
|
S. Jimi, N. Uesugi, K. Saku, H. Itabe, B. Zhang, K. Arakawa, and S. Takebayashi Possible Induction of Renal Dysfunction in Patients With Lecithin:Cholesterol Acyltransferase Deficiency by Oxidized Phosphatidylcholine in Glomeruli Arterioscler. Thromb. Vasc. Biol., March 1, 1999; 19(3): 794 - 801. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Freigang, S. Horkko, E. Miller, J. L. Witztum, and W. Palinski Immunization of LDL Receptor–Deficient Mice With Homologous Malondialdehyde-Modified and Native LDL Reduces Progression of Atherosclerosis by Mechanisms Other Than Induction of High Titers of Antibodies to Oxidative Neoepitopes Arterioscler. Thromb. Vasc. Biol., December 1, 1998; 18(12): 1972 - 1982. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Steinberg Low Density Lipoprotein Oxidation and Its Pathobiological Significance J. Biol. Chem., August 22, 1997; 272(34): 20963 - 20966. [Full Text] [PDF]
|
|
|
|
|
|
O. Tamai, H. Matsuoka, H. Itabe, Y. Wada, K. Kohno, and T. Imaizumi Single LDL Apheresis Improves Endothelium-Dependent Vasodilatation in Hypercholesterolemic Humans Circulation, January 7, 1997; 95(1): 76 - 82. [Abstract] [Full Text]
|
|
|
|
|
|
H. Itabe, H. Yamamoto, M. Suzuki, Y. Kawai, Y. Nakagawa, A. Suzuki, T. Imanaka, and T. Takano Oxidized Phosphatidylcholines That Modify Proteins. ANALYSIS BY MONOCLONAL ANTIBODY AGAINST OXIDIZED LOW DENSITY LIPOPROTEIN J. Biol. Chem., December 27, 1996; 271(52): 33208 - 33217. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Tanaga, H. Bujo, M. Inoue, K. Mikami, K. Kotani, K. Takahashi, T. Kanno, and Y. Saito Increased Circulating Malondialdehyde-Modified LDL Levels in Patients With Coronary Artery Diseases and Their Association With Peak Sizes of LDL Particles Arterioscler. Thromb. Vasc. Biol., April 1, 2002; 22(4): 662 - 666. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |
