HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kern, P. A. Articles by Davis, R. C. Search for Related Content PubMed PubMed Citation Articles by Kern, P. A. Articles by Davis, R. C. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 37, 2332-2340, Copyright © 1996 by Lipid Research, Inc.

ARTICLES

Translational regulation of lipoprotein lipase by thyroid hormone is via a cytoplasmic repressor that interacts with the 3' untranslated region

PA Kern, G Ranganathan, A Yukht, JM Ong and RC Davis
Department of Medicine, University of Arkansas for Medical Sciences, Little Rock, USA.

To better characterize the increase in lipoprotein lipase (LPL) translation by hypothyroidism, adipocytes were prepared from control and hypothyroid rats. Whereas LPL synthesis was higher in hypothyroid adipocytes, with no change in mRNA levels, there was no increase in hormone-sensitive lipase (HSL) synthesis. To determine whether a transacting translation regulatory factor was present, a cytoplasmic fraction was prepared from control and hypothyroid adipocytes, and added to an in vitro translation system containing the hLPL mRNA. The hypothyroid cell fraction from adipose and heart yielded an increase in LPL translation, when compared to control extracts. Further experiments determined that the control adipocyte extract contained a translation- inhibitory factor that was 8-fold lower in activity in the hypothyroid extract. Using different LPL mRNA constructs in the in vitro translation reaction, the region that controlled translation was localized to nucleotides 1599 to 1638 (proximal 3' untranslated region (UTR)). To confirm the presence of a transacting factor, a sense RNA strand corresponding to this region was added to the in vitro translation reaction. This sense strand competed for the transacting factor in the control cell extract, yet had no effect on the hypothyroid cell extract. Thus, there is a translation repressor factor in the cytoplasm of rat adipocytes, and this factor is greatly reduced in activity in hypothyroid rat adipocytes. Because a similar mechanism of LPL regulation occurs in response to epinephrine, the absence of the translation repressor may be a mechanism for the loss of sensitivity of hypothyroid cells for catecholamines.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J. Polak, C. Moro, E. Klimcakova, M. Kovacikova, M. Bajzova, M. Vitkova, Z. Kovacova, R. Sotornik, M. Berlan, N. Viguerie, et al. The atrial natriuretic peptide- and catecholamine-induced lipolysis and expression of related genes in adipose tissue in hypothyroid and hyperthyroid patients Am J Physiol Endocrinol Metab, July 1, 2007; 293(1): E246 - E251. [Abstract] [Full Text] [PDF]

				X. Prieur, T. Huby, H. Coste, F. G. Schaap, M. J. Chapman, and J. C. Rodriguez Thyroid Hormone Regulates the Hypotriglyceridemic Gene APOA5 J. Biol. Chem., July 29, 2005; 280(30): 27533 - 27543. [Abstract] [Full Text] [PDF]

				M. Haluzik, J. Nedvidkova, V. Bartak, I. Dostalova, P. Vlcek, P. Racek, M. Taus, S. Svacina, S. Alesci, and K. Pacak Effects of Hypo- and Hyperthyroidism on Noradrenergic Activity and Glycerol Concentrations in Human Subcutaneous Abdominal Adipose Tissue Assessed with Microdialysis J. Clin. Endocrinol. Metab., December 1, 2003; 88(12): 5605 - 5608. [Abstract] [Full Text] [PDF]

				L. L. Hensley, G. Ranganathan, E. M. Wagner, B. D. Wells, J. C. Daniel, D. Vu, C. F. Semenkovich, R. Zechner, and P. A. Kern Transgenic Mice Expressing Lipoprotein Lipase in Adipose Tissue: ABSENCE OF THE PROXIMAL 3'-UNTRANSLATED REGION CAUSES TRANSLATIONAL UPREGULATION J. Biol. Chem., August 29, 2003; 278(35): 32702 - 32709. [Abstract] [Full Text] [PDF]

				G. Ranganathan, D. Phan, I. D. Pokrovskaya, J. E. McEwen, C. Li, and P. A. Kern The Translational Regulation of Lipoprotein Lipase by Epinephrine Involves an RNA Binding Complex Including the Catalytic Subunit of Protein Kinase A J. Biol. Chem., November 1, 2002; 277(45): 43281 - 43287. [Abstract] [Full Text] [PDF]

				G. Ranganathan, W. Song, N. Dean, B. Monia, S. W. Barger, and P. A. Kern Regulation of Lipoprotein Lipase by Protein Kinase Calpha in 3T3-F442A Adipocytes J. Biol. Chem., October 4, 2002; 277(41): 38669 - 38675. [Abstract] [Full Text] [PDF]

				H.-C. Li, D. Liu, and D. J. Waxman Transcriptional Induction of Hepatic NADPH: Cytochrome P450 Oxidoreductase by Thyroid Hormone Mol. Pharmacol., April 16, 2001; 59(5): 987 - 995. [Abstract] [Full Text]

				S. E. Michaud and G. Renier Direct Regulatory Effect of Fatty Acids on Macrophage Lipoprotein Lipase: Potential Role of PPARs Diabetes, March 1, 2001; 50(3): 660 - 666. [Abstract] [Full Text]

				P. J. A. Davies, S. A. Berry, G. L. Shipley, R. H. Eckel, N. Hennuyer, D. L. Crombie, K. M. Ogilvie, J. Peinado-Onsurbe, C. Fievet, M. D. Leibowitz, et al. Metabolic Effects of Rexinoids: Tissue-Specific Regulation of Lipoprotein Lipase Activity Mol. Pharmacol., February 1, 2001; 59(2): 170 - 176. [Abstract] [Full Text]

				E. G. M. Mbella, S. Bertrand, G. Huez, and J.-N. Octave A GG Nucleotide Sequence of the 3' Untranslated Region of Amyloid Precursor Protein mRNA Plays a Key Role in the Regulation of Translation and the Binding of Proteins Mol. Cell. Biol., July 1, 2000; 20(13): 4572 - 4579. [Abstract] [Full Text]

				G. Ranganathan, R. Kaakaji, and P. A. Kern Role of Protein Kinase C in the Translational Regulation of Lipoprotein Lipase in Adipocytes J. Biol. Chem., March 26, 1999; 274(13): 9122 - 9127. [Abstract] [Full Text] [PDF]

				A. Stutz, B. Conne, J. Huarte, P. Gubler, V. Völkel, P. Flandin, and J.-D. Vassalli Masking, unmasking, and regulated polyadenylation cooperate in the translational control of a dormant mRNA in mouse oocytes Genes & Dev., August 15, 1998; 12(16): 2535 - 2548. [Abstract] [Full Text]

				T. Scantlebury, M. Maslowska, and K. Cianflone Chylomicron-specific Enhancement of Acylation Stimulating Protein and Precursor Protein C3 Production in Differentiated Human Adipocytes J. Biol. Chem., August 14, 1998; 273(33): 20903 - 20909. [Abstract] [Full Text] [PDF]

				G. Ranganathan, C. Li, and P. A. Kern The Translational Regulation of Lipoprotein Lipase in Diabetic Rats Involves the 3'-Untranslated Region of the Lipoprotein Lipase mRNA J. Biol. Chem., December 22, 2000; 275(52): 40986 - 40991. [Abstract] [Full Text] [PDF]