| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Journal of Lipid Research, Vol 37, 2341-2350, Copyright © 1996 by Lipid Research, Inc.
CJ Walkey, Z Cui, LB Agellon and DE Vance
Phosphatidylethanolamine N-methyltransferase (PEMT) catalyzes the
conversion of phosphatidylethanolamine to phosphatidylcholine in the
mammalian liver via three sequential methylations. In the present studies,
we cloned and characterized the murine gene for PEMT2, the isoform of the
enzyme that localized to the mitochondria-associated membrane. The
structure of the gene was determined by analysis of two lambda and three P1
genomic clones, and compared to the known rat PEMT2 cDNA sequence. Southern
blotting of mouse genomic DNA indicated that PEMT2 is a single-copy gene.
The gene spans at least 35 kb, with seven exons and six introns. Two
transcription start sites, 139 and 148 base pairs upstream of the
translation start site, were detected by primer extension and reverse
transcriptase-polymerase chain reaction. These experiments indicated that
the PEMT2 gene is transcribed from a single promoter. Finally, the PEMT2
gene was localized to mouse chromosome 11 by interspecific backcrossing.
These experiments represent the first cloning and characterization of a
full-length mammalian gene involved in phospholipid biosynthesis.
ARTICLES
Characterization of the murine phosphatidylethanolamine N- methyltransferase-2 gene
Department of Biochemistry, University of Alberta, Edmonton, Canada.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  Z. Li and D. E. Vance Thematic Review Series: Glycerolipids. Phosphatidylcholine and choline homeostasis J. Lipid Res., June 1, 2008; 49(6): 1187 - 1194. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. E. Vance, Z. Li, and R. L. Jacobs Hepatic Phosphatidylethanolamine N-Methyltransferase, Unexpected Roles in Animal Biochemistry and Physiology J. Biol. Chem., November 16, 2007; 282(46): 33237 - 33241. [Full Text] [PDF]
|
|
|
|
 |
|
 M. Resseguie, J. Song, M. D. Niculescu, K.-A. da Costa, T. A. Randall, and S. H. Zeisel Phosphatidylethanolamine N-methyltransferase (PEMT) gene expression is induced by estrogen in human and mouse primary hepatocytes FASEB J, August 1, 2007; 21(10): 2622 - 2632. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Noga and D. E. Vance Insights into the requirement of phosphatidylcholine synthesis for liver function in mice J. Lipid Res., October 1, 2003; 44(10): 1998 - 2005. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Bi, J. Yan, P. Stankiewicz, S.-S. Park, K. Walz, C. F. Boerkoel, L. Potocki, L. G. Shaffer, K. Devriendt, M. J.M. Nowaczyk, et al. Genes in a Refined Smith-Magenis Syndrome Critical Deletion Interval on Chromosome 17p11.2 and the Syntenic Region of the Mouse Genome Res., May 1, 2002; 12(5): 713 - 728. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Tessitore, E. Sesca, M. Bosco, and D. E. Vance Expression of phosphatidylethanolamine N-methyltransferase in Yoshida ascites hepatoma cells and the livers of host rats Carcinogenesis, April 1, 1999; 20(4): 561 - 567. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. J. Walkey, L. R. Donohue, R. Bronson, L. B. Agellon, and D. E. Vance Disruption of the murine gene encoding phosphatidylethanolamine N-methyltransferase PNAS, November 25, 1997; 94(24): 12880 - 12885. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Tang, G. A. Keesler, and I. Tabas The Structure of the Gene for Murine CTP:Phosphocholine Cytidylyltransferase, Ctpct. RELATIONSHIP OF EXON STRUCTURE TO FUNCTIONAL DOMAINS AND IDENTIFICATION OF TRANSCRIPTIONAL START SITES AND POTENTIAL UPSTREAM REGULATORY ELEMENTS J. Biol. Chem., May 16, 1997; 272(20): 13146 - 13151. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |
