|
 |
|
|||||||
|
|||||||||
Journal of Lipid Research, Vol 37, 275-289, Copyright © 1996 by Lipid Research, Inc.
A Sjoberg, J Oscarsson, J Boren, S Eden and SO Olofsson
Hypophysectomized female rats were treated for 1 week with thyroxine (10
micrograms/kg.day), cortisol (400 micrograms/kg.day), and bovine GH (1
mg/kg.day) either as two daily subcutaneous injections (GH x 2) or as a
continuous subcutaneous infusion (GHc) in order to mimic the male and
female specific GH secretory patterns, respectively. Hepatocytes were then
isolated and kept in short-term cultures. Hypophysectomy decreased the
synthesis of triacylglycerol. Treatment with GH x 2 had no or small
effects, while GHc normalized the effect of hypophysectomy. ApoB-100 VLDL
was assembled before apoB-48 VLDL. ApoB-48 was first assembled as an HDL
particle (apoB-48 "HDL"). Hypophysectomy decreased the proportion of
intracellular apoB-48 that was recovered as VLDL. Moreover, the proportion
of apoB-48 of total apoB in VLDL decreased. Only GHc fully restored the
effect of hypophysectomy by inducing an 4- fold increase in the assembly of
apoB-48 VLDL, while treatment with GH x 2 gave rise to a 1.8-fold increase.
Hypophysectomy resulted in a decrease in the proportion of apoB-48 that was
secreted as VLDL and a decrease in the proportion of apoB-48 of total apoB
in VLDL. Only treatment with GHc fully restored the secretion of apoB-48
VLDL by inducing an almost 4-fold increase in the secretion of apoB-48
VLDL, while the corresponding value for treatment with GH x 2 was 1.7.
However, GH x 2 increased the proportion of the secreted apoB-48 that was
recovered in VLDL to the levels found in normal rats and in rats treated
with GHc, but this finding was due to a failure of GH x 2 treatment to
increase the secretion of apoB-48 "HDL". In summary, a continuous infusion
of GH to hypophysectomized rats, mimicking the female secretion of GH,
normalized the triacylglycerol synthesis and secretion as well as apoB-48
VLDL assembly and secretion to those levels observed in hepatocyte cultures
from intact female rats.
ARTICLES
Mode of growth hormone administration influences triacylglycerol synthesis and assembly of apolipoprotein B-containing lipoproteins in cultured rat hepatocytes
Department of Medical Biochemistry, Goteborg University, Sweden.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  C. Ameen, D. Linden, B.-M. Larsson, A. Mode, A. Holmang, and J. Oscarsson Effects of gender and GH secretory pattern on sterol regulatory element-binding protein-1c and its target genes in rat liver Am J Physiol Endocrinol Metab, December 1, 2004; 287(6): E1039 - E1048. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Ameen and J. Oscarsson Sex Difference in Hepatic Microsomal Triglyceride Transfer Protein Expression Is Determined by the Growth Hormone Secretory Pattern in the Rat Endocrinology, September 1, 2003; 144(9): 3914 - 3921. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Olsson, M. Bohlooly-Y, O. Brusehed, O. G. P. Isaksson, B. Ahren, S.-O. Olofsson, J. Oscarsson, and J. Tornell Bovine growth hormone-transgenic mice have major alterations in hepatic expression of metabolic genes Am J Physiol Endocrinol Metab, September 1, 2003; 285(3): E504 - E511. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Jalouli, L. Carlsson, C. Ameen, D. Linden, A. Ljungberg, L. Michalik, S. Eden, W. Wahli, and J. Oscarsson Sex Difference in Hepatic Peroxisome Proliferator-Activated Receptor {alpha} Expression: Influence of Pituitary and Gonadal Hormones Endocrinology, January 1, 2003; 144(1): 101 - 109. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Frick, D. Linden, C. Ameen, S. Eden, A. Mode, and J. Oscarsson Interaction between growth hormone and insulin in the regulation of lipoprotein metabolism in the rat Am J Physiol Endocrinol Metab, November 1, 2002; 283(5): E1023 - E1031. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Nishimaki-Mogami, Z. Yao, and K. Fujimori Inhibition of phosphatidylcholine synthesis via the phosphatidylethanolamine methylation pathway impairs incorporation of bulk lipids into VLDL in cultured rat hepatocytes J. Lipid Res., July 1, 2002; 43(7): 1035 - 1045. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Linden, K. Lindberg, J. Oscarsson, C. Claesson, L. Asp, L. Li, M. Gustafsson, J. Boren, and S.-O. Olofsson Influence of Peroxisome Proliferator-activated Receptor alpha Agonists on the Intracellular Turnover and Secretion of Apolipoprotein (Apo) B-100 and ApoB-48 J. Biol. Chem., June 14, 2002; 277(25): 23044 - 23053. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Frick, M. Bohlooly-Y, D. Linden, B. Olsson, J. Tornell, S. Eden, and J. Oscarsson Long-term growth hormone excess induces marked alterations in lipoprotein metabolism in mice Am J Physiol Endocrinol Metab, December 1, 2001; 281(6): E1230 - E1239. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Oscarsson, C. Gardmo, S. Eden, and A. Mode Pulsatile growth hormone secretion decreases S-adenosylmethionine synthetase in rat liver Am J Physiol Endocrinol Metab, February 1, 2001; 280(2): E280 - E286. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Linden, A. Sjoberg, L. Asp, L. Carlsson, and J. Oscarsson Direct effects of growth hormone on production and secretion of apolipoprotein B from rat hepatocytes Am J Physiol Endocrinol Metab, December 1, 2000; 279(6): E1335 - E1346. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Leonsson, J. Oscarsson, I. Bosaeus, B. K. Lundgren, G. Johannsson, O. Wiklund, and B. A. Bengtsson Growth Hormone (GH) Therapy in GH-Deficient Adults Influences the Response to a Dietary Load of Cholesterol and Saturated Fat in Terms of Cholesterol Synthesis, But Not Serum Low Density Lipoprotein Cholesterol Levels J. Clin. Endocrinol. Metab., April 1, 1999; 84(4): 1296 - 1303. [Abstract] [Full Text]
|
|
|
|
|
|
L. Carlsson, I. Nilsson, and J. Oscarsson Hormonal Regulation of Liver Fatty Acid-Binding Protein in Vivo and in Vitro: Effects of Growth Hormone and Insulin Endocrinology, June 1, 1998; 139(6): 2699 - 2709. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Rustaeus, P. Stillemark, K. Lindberg, D. Gordon, and S.-O. Olofsson The Microsomal Triglyceride Transfer Protein Catalyzes the Post-translational Assembly of Apolipoprotein B-100 Very Low Density Lipoprotein in McA-RH7777 Cells J. Biol. Chem., February 27, 1998; 273(9): 5196 - 5203. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Adeli, M. Wettesten, L. Asp, A. Mohammadi, J. Macri, and S.-O. Olofsson Intracellular Assembly and Degradation of Apolipoprotein B-100-containing Lipoproteins in Digitonin-permeabilized HEP G2 Cells J. Biol. Chem., February 21, 1997; 272(8): 5031 - 5039. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Tran, Y. Wang, C. J. DeLong, Z. Cui, and Z. Yao The Assembly of Very Low Density Lipoproteins in Rat Hepatoma McA-RH7777 Cells Is Inhibited by Phospholipase A2 Antagonists J. Biol. Chem., August 4, 2000; 275(32): 25023 - 25030. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |