HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hebuterne, X. Articles by Russell, R. M. Search for Related Content PubMed PubMed Citation Articles by Hebuterne, X. Articles by Russell, R. M. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 37, 482-492, Copyright © 1996 by Lipid Research, Inc.

ARTICLES

In vivo biosynthesis of retinoic acid from beta-carotene involves and excentric cleavage pathway in ferret intestine

X Hebuterne, XD Wang, DE Smith, G Tang and RM Russell
United States Department of Agriculture, Human Nutrition Research Center on Aging, Tufts University, Boston, MA 02111, USA.

This study was done to determine whether retinoic acid can be produced by excentric cleavage of beta-carotene in vivo. By using an inhibitor of retinaldehyde oxidation, citral, either retinaldehyde or beta- carotene was incorporated in a micellar solution and perfused through the upper portion of small intestine of ferrets. After 2 h perfusion of 1 microM retinaldehyde, retinoic acid rose in portal blood (+3.5 +/- 1.3 nmol/L) and was detected in the intestinal mucosa (30 +/- 2 pmol/g). When citral was added at 2 mM along with retinaldehyde, retinoic acid decreased in the portal blood and retinoic acid was not detected in the intestinal mucosa. With or without the presence of citral (2 mM), the perfusion of beta-carotene (10 microM) during 2 h caused a significant rise of retinoic acid in portal blood (+2.6 +/- 0.6 nmol/L and + 4.1 +/- 0.6 nmol/L, respectively) and in liver; moreover, significant amounts of retinoic acid were detected in the intestinal mucosa (19 +/- 3 pmol/g and 36 +/- pmol/g, respectively. This study demonstrates that after intestinal perfusion of beta- carotene in the ferret in vivo, a substantial amount of retinoic acid is formed via an excentric cleavage pathway.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				I. R. Preston, G. Tang, J. U. Tilan, N. S. Hill, and Y. J. Suzuki Retinoids and Pulmonary Hypertension Circulation, February 15, 2005; 111(6): 782 - 790. [Abstract] [Full Text] [PDF]

				P. Prakash, C. Liu, K.-Q. Hu, N. I. Krinsky, R. M. Russell, and X.-D. Wang {beta}-Carotene and {beta}-Apo-14'-Carotenoic Acid Prevent the Reduction of Retinoic Acid Receptor {beta} in Benzo[a]pyrene-Treated Normal Human Bronchial Epithelial Cells J. Nutr., March 1, 2004; 134(3): 667 - 673. [Abstract] [Full Text] [PDF]

				S. L. Lemke, S. R. Dueker, J. R. Follett, Y. Lin, C. Carkeet, B. A. Buchholz, J. S. Vogel, and A. J. Clifford Absorption and retinol equivalence of {beta}-carotene in humans is influenced by dietary vitamin A intake J. Lipid Res., August 1, 2003; 44(8): 1591 - 1600. [Abstract] [Full Text] [PDF]

				T. Arnhold, H. Nau, S. Meyer, H. J. Rothkoetter, and A. D. Lampen Porcine Intestinal Metabolism of Excess Vitamin A Differs Following Vitamin A Supplementation and Liver Consumption J. Nutr., February 1, 2002; 132(2): 197 - 203. [Abstract] [Full Text] [PDF]

				X.-D. Wang, R. M. Russell, C. Liu, F. Stickel, D. E. Smith, and N. I. Krinsky beta -Oxidation in Rabbit Liver in Vitro and in the Perfused Ferret Liver Contributes to Retinoic Acid Biosynthesis from beta -Apocarotenoic Acids J. Biol. Chem., October 25, 1996; 271(43): 26490 - 26498. [Abstract] [Full Text] [PDF]