HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Arduini, A. Articles by Calvani, M. Search for Related Content PubMed PubMed Citation Articles by Arduini, A. Articles by Calvani, M. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 37, 684-689, Copyright © 1996 by Lipid Research, Inc.

ARTICLES

High performance liquid chromatography of long-chain acylcarnitine and phospholipids in fatty acid turnover studies

A Arduini, A Peschechera, S Dottori, AF Sciarroni, F Serafini and M Calvani
Department of Sciences, University of Chieti, Italy.

In this paper we describe a rapid, isocratic high performance liquid chromatography (HPLC) method for the study of radioactive fatty acid incorporation into complex lipids of human erythrocytes, which allows the simultaneous separation of the major phospholipid classes and long- chain acylcarnitines. The lipid extract of erythrocytes pulsed with radioactive fatty acids was injected into an HPLC system equipped with a silica column. The individual components eluted were monitored by ultraviolet absorption and radioactive emission. With respect to the UV profile, the radioactive profile showed an additional peak between phosphatidyl-choline and phosphatidylethanolamine, which was identified as long-chain acylcarnitine by different experimental approaches. The radioactivity recovered in the long-chain acylcarnitines contains essential information enabling definition of acyl trafficking in red cells.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				D. Sun, M. G. Cree, X.-j. Zhang, E. Boersheim, and R. R. Wolfe Measurement of stable isotopic enrichment and concentration of long-chain fatty acyl-carnitines in tissue by HPLC-MS J. Lipid Res., February 1, 2006; 47(2): 431 - 439. [Abstract] [Full Text] [PDF]

				S. N. Hawk, L. Lanoue, C. L. Keen, C. L. Kwik-Uribe, R. B. Rucker, and J. Y. Uriu-Adams Copper-Deficient Rat Embryos Are Characterized by Low Superoxide Dismutase Activity and Elevated Superoxide Anions Biol Reprod, March 1, 2003; 68(3): 896 - 903. [Abstract] [Full Text] [PDF]