Journal of Lipid Research, Vol 37, 877-883, Copyright © 1996 by Lipid Research, Inc.
A novel vesicular assay to study factors affecting cholesterol crystallization in vitro
AI Zijlstra, BJ Van de Heijning, M van Overveld and AK Groen
Department of Gastroenterology and Hepatology, Academic Medical Center, Amsterdam, The Netherlands.
We present an assay to study cholesterol crystallization that is fast,
facile, and highly reproducible. Cholesterol crystallization from
metastable vesicles was induced upon addition of bile salts and depended on
the hydrophobicity of the bile salt used and the
cholesterol-to-phospholipid ratio of the vesicles. Bile salt-induced
crystallization was stimulated upon addition of Con A-binding glycoproteins
(CABGs), comparable to the results of the same CABGs in a crystal growth
assay. The onset time and total measuring time, however, were much shorter.
This assay might, moreover, provide a tool to study the mechanism of
cholesterol crystallization in more detail.