HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Curtiss, L. K. Articles by Banka, C. L. Search for Related Content PubMed PubMed Citation Articles by Curtiss, L. K. Articles by Banka, C. L. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 37, 884-892, Copyright © 1996 by Lipid Research, Inc.

ARTICLES

Selection of monoclonal antibodies for linear epitopes of an apolipoprotein yields antibodies with comparable affinity for lipid- free and lipid-associated apolipoprotein

LK Curtiss and CL Banka
Department of Immunology, Scripps Research Institute, La Jolla, CA 92037, USA.

A direct approach is described for generating human apolipoprotein (apo) A-I-specific monoclonal antibodies with defined linear epitopes. The antibodies have comparable binding affinity for delipidated apoA-I and apoA-I on high density lipoproteins (HDL). The antibodies were obtained by immunizing mice with HDL, screening the fusions first for antibodies that bind native HDL and second, for antibodies that bind defined apoA-I synthetic peptides. ApoA-I antibody epitopes assigned on the basis of synthetic peptide binding were confirmed by solid phase and fluid phase antibody competition assays. These antibodies, which bind epitopes that encompass greater than 60% of apoA-I, should prove useful for identifying discrete functional domains of apoA-I on HDL.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				C. M. Desrumaux, P. A. Mak, W. A. Boisvert, D. Masson, D. Stupack, M. Jauhiainen, C. Ehnholm, and L. K. Curtiss Phospholipid transfer protein is present in human atherosclerotic lesions and is expressed by macrophages and foam cells J. Lipid Res., August 1, 2003; 44(8): 1453 - 1461. [Abstract] [Full Text] [PDF]

				J. W. Chisholm, E. R. Burleson, G. S. Shelness, and J. S. Parks ApoA-I secretion from HepG2 cells: evidence for the secretion of both lipid-poor apoA-I and intracellularly assembled nascent HDL J. Lipid Res., January 1, 2002; 43(1): 36 - 44. [Abstract] [Full Text] [PDF]

				C. V. Zerbinatti, L. P. Mayer, R. G. Audet, and C. A. Dyer Apolipoprotein E Is a Putative Autocrine Regulator of the Rat Ovarian Theca Cell Compartment Biol Reprod, April 1, 2001; 64(4): 1080 - 1089. [Abstract] [Full Text]

				M. G. Sorci-Thomas, M. Thomas, L. Curtiss, and M. Landrum Single Repeat Deletion in ApoA-I Blocks Cholesterol Esterification and Results in Rapid Catabolism of Delta 6 and Wild-type ApoA-I in Transgenic Mice J. Biol. Chem., April 14, 2000; 275(16): 12156 - 12163. [Abstract] [Full Text] [PDF]

				K. W. Huggins, L. K. Curtiss, A. K. Gebre, and J. S. Parks Effect of long chain polyunsaturated fatty acids in the sn-2 position of phosphatidylcholine on the interaction with recombinant high density lipoprotein apolipoprotein A-I J. Lipid Res., December 1, 1998; 39(12): 2423 - 2431. [Abstract] [Full Text]

				M. G. Sorci-Thomas, L. Curtiss, J. S. Parks, M. J. Thomas, M. W. Kearns, and M. Landrum The Hydrophobic Face Orientation of Apolipoprotein A-I Amphipathic Helix Domain 143-164 Regulates Lecithin:Cholesterol Acyltransferase Activation J. Biol. Chem., May 8, 1998; 273(19): 11776 - 11782. [Abstract] [Full Text] [PDF]

				M. G. Sorci-Thomas, L. Curtiss, J. S. Parks, M. J. Thomas, and M. W. Kearns Alteration in Apolipoprotein A-I 22-Mer Repeat Order Results in a Decrease in Lecithin:Cholesterol Acyltransferase Reactivity J. Biol. Chem., March 14, 1997; 272(11): 7278 - 7284. [Abstract] [Full Text] [PDF]