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Journal of Lipid Research, Vol 37, 902-904, Copyright © 1996 by Lipid Research, Inc.

ARTICLES

Apolipoprotein H: a two-step isolation method

R Gambino, G Ruiu, M Cassader and G Pagano
Istituto di Medicina Interna, Universita di Torino, Italy.

A new method for the purification of apolipoprotein H by affinity chromatography followed by continuous-elution electrophoresis is described. It is both simpler and less complicated than the chromatographic and electrophoretic methods usually used. In addition, apolipoprotein H is isolated in a pure, structurally uncleaved form. This is of importance, as impairment has been detected in commercial preparations. The separation and purification of apolipoprotein H is a necessary prelude to its quantitative determination and phenotyping, and hence the clarification of its physiopathological mechanisms in lipid metabolism.
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