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Journal of Lipid Research, Vol 37, 1022-1036, Copyright © 1996 by Lipid Research, Inc.
M Hultin, R Savonen and T Olivecrona
Chylomicrons labeled in vivo with [14C]oleic acid (primarily in
triglycerides (TG), providing a tracer for lipolysis) and [3H]retinol
(primarily in ester form, providing a tracer for the corelipids) were
injected into rats. Disappearance of the two labels from plasma and
appearance of label in plasma free fatty acids (FFA) were analyzed by
compartmental modelling. Both core and TG label distributed into an
apparent volume 10-15% larger than the blood volume. Part of this probably
represents margination to endothelial-binding-lipolysis sites. An open
two-compartmental model for plasma FFA was derived from experiments where
unesterified oleic acid complexed to albumin was injected. Applying this
model revealed that most of the oleic acid from chylomicron triglycerides
mixes with the FFA. The disappearance of chylomicron core label required a
model in which the label transfers into a second compartment before it
leaves the blood. The rate constant for the transformation was high and
predicted that, on average, chylomicron spent less than 2 min in the first
compartment. The rate out from the second compartment predicted that about
60% of the core label left blood while, on average, chylomicron retained
more than half of its triglyceride molecules, i.e., after rather limited
lipolysis. The mechanism by which the core label leaves blood is not clear.
Modelling showed that under the assumption that the process is shared by
chylomicron triglycerides, about half of them go out by this pathway.
Comparing fed and fasted rats, the main differences were in the turnover of
FFA and in the extent to which chylomicron TG label reappeared in the FFA.
This study indicates that a large fraction of the triglycerides in
chylomicrons leave plasma together with the core lipids and that most of
the fatty acids from chylomicron triglycerides mix into the same metabolic
compartments as do plasma free fatty acids.
ARTICLES
Chylomicron metabolism in rats: lipolysis, recirculation of triglyceride-derived fatty acids in plasma FFA, and fate of core lipids as analyzed by compartmental modelling
Department of Medical Biochemistry and Biophysics, University of Umea, Sweden.
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