| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Journal of Lipid Research, Vol 37, 1057-1064, Copyright © 1996 by Lipid Research, Inc.
AB Fisher and C Dodia
The role of phospholipase A2 (PLA2) enzymes in the degradation of
internalized dipalmitoylphospharidylcoline (DPPC) by rat granular
pneumocytes was evaluated with cells in 24 h primary culture on microporous
membranes. In cell sonicates and rat lung homogenates, the transition state
analogue MJ33 inhibited acidic (pH 4), Ca(2+)- independent PLA2 (aiPLA2)
while p-bromophenacylbromide (pBPB) inhibited alkaline (pH 8.5),
Ca(2+)-dependent PLA2 and phospholipase C activities. With intact cells,
degradation of [3H]methylcholine-labeled DPPC during 2 h incubation was
inhibited 48% by MJ33, 20% by pBPB, and 69%by the combination. The
inhibitors (20 microM pBPB, 3 mol% MJ33) had no effect on cellular dye
exclusion, adherence to membranes, or uptake of DPPC. Arachidonyl
trifuoromethylketone, a cytoplasmic PLA2 inhibitor, had no effect on
cellular degradation of DPPC. Degradation was depressed approximately 20%
by the addition of NH4Cl or methylamine to the medium, suggesting a role
for an acidic intracellular compartment in DPPC metabolism. Subcellular
fractions prepared by differential centrifugation of rat lung homogenates
showed highest specific activity of aiPLA2 in the lamellar body and
lysosomal fractions, lower activity in cytosol, and essentially no activity
in mitochondria, microsomes, or plasma membranes. The results of this study
indicate that aiPLA2 has the major role in the degradation of internalized
DPPC by granular pneumocytes and they are compatible with participation of
lysosomes/lamellar bodies in DPPC metabolism.
ARTICLES
Role of phospholipase A2 enzymes in degradation of dipalmitoylphosphatidylcholine by granular pneumocytes
Institute for Environmental Medicine, University of Pennsylvania Medical Center, Philadelphia 19104, USA.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. Manevich, K. S. Reddy, T. Shuvaeva, S. I. Feinstein, and A. B. Fisher Structure and phospholipase function of peroxiredoxin 6: identification of the catalytic triad and its role in phospholipid substrate binding J. Lipid Res., October 1, 2007; 48(10): 2306 - 2318. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-Z. Wu, Y. Manevich, J. L. Baldwin, C. Dodia, K. Yu, S. I. Feinstein, and A. B. Fisher Interaction of Surfactant Protein A with Peroxiredoxin 6 Regulates Phospholipase A2 Activity J. Biol. Chem., March 17, 2006; 281(11): 7515 - 7525. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. B. Fisher, C. Dodia, S. I. Feinstein, and Y.-S. Ho Altered lung phospholipid metabolism in mice with targeted deletion of lysosomal-type phospholipase A2 J. Lipid Res., June 1, 2005; 46(6): 1248 - 1256. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. B. Fisher and C. Dodia Lysosomal-type PLA2 and turnover of alveolar DPPC Am J Physiol Lung Cell Mol Physiol, April 1, 2001; 280(4): L748 - L754. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. G. Salome, D. M. McCoy, A. J. Ryan, and R. K. Mallampalli Effects of intratracheal instillation of TNF-alpha on surfactant metabolism J Appl Physiol, January 1, 2000; 88(1): 10 - 16. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Chen, A. B. Fisher, D. S. Strayer, and S. R. Bates Mechanism for secretagogue-induced surfactant protein A binding to lung epithelial cells Am J Physiol Lung Cell Mol Physiol, July 1, 1998; 275(1): L38 - L46. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T.-S. Kim, C. Dodia, X. Chen, B. B. Hennigan, M. Jain, S. I. Feinstein, and A. B. Fisher Cloning and expression of rat lung acidic Ca2+-independent PLA2 and its organ distribution Am J Physiol Lung Cell Mol Physiol, May 1, 1998; 274(5): L750 - L761. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. R. Bates, J. Xu, C. Dodia, and A. B. Fisher Macrophages primed by overnight culture demonstrate a marked stimulation of surfactant protein A degradation Am J Physiol Lung Cell Mol Physiol, October 1, 1997; 273(4): L831 - L839. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T.-S. Kim, C. S. Sundaresh, S. I. Feinstein, C. Dodia, W. R. Skach, M. K. Jain, T. Nagase, N. Seki, K.-i. Ishikawa, N. Nomura, et al. Identification of a Human cDNA Clone for Lysosomal Type Ca2+-independent Phospholipase A2 and Properties of the Expressed Protein J. Biol. Chem., January 24, 1997; 272(4): 2542 - 2550. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-W. Chen, C. Dodia, S. I. Feinstein, M. K. Jain, and A. B. Fisher 1-Cys Peroxiredoxin, a Bifunctional Enzyme with Glutathione Peroxidase and Phospholipase A2 Activities J. Biol. Chem., September 8, 2000; 275(37): 28421 - 28427. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |
