HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Robinson, B. S. Articles by Ferrante, A. Search for Related Content PubMed PubMed Citation Articles by Robinson, B. S. Articles by Ferrante, A. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 37, 1234-1245, Copyright © 1996 by Lipid Research, Inc.

ARTICLES

Effect of tumor necrosis factor-alpha on the metabolism of arachidonic acid in human neutrophils

BS Robinson, CS Hii, A Poulos and A Ferrante
Department of Immunology, Women's and Children's Hospital, North Adelaide, Australia.

Although tumor necrosis factor-alpha (TNF-alpha) has been shown to induce marked changes in the physiology/pathophysiology of cells, little is known about the effects of this cytokine on cellular lipid metabolism. In this study we examined the effects of TNF-alpha on the metabolism of eicosatetraenoic acid (arachidonic acid, (20:4(n-6)) in human neutrophils. Pretreatment of neutrophils with TNF-alpha caused a rapid increase in the incorporation of [1-14C]20:4(n-6) substrate into cellular phosphatidylinositol and phosphatidic acid and a slower rise in the incorporation into phosphatidylcholine and phosphatidylethanolamine. Radioactivity was exclusively associated with the sn-2 position of each molecule. The labeling pattern of other phospholipids, neutral lipids, and eicosanoids was unchanged. TNF-alpha had no effect on the distribution of radioactivity in 1-acyl, 1-alkyl, and 1-alk-1-enyl subclasses of phosphatidylcholine, phosphatidylethanolamine, and triglyceride. Chain elongation, beta- oxidation and desaturation of [1-14C]20:4(n-6) were not modulated by the cytokine. TNF-alpha stimulated the release of [3H]20:4(n-6) from prelabeled neutrophils and also induced the production of endogenous unesterified 20:4(n-6). Concomitantly, treatment with the cytokine caused a decrease in the mass of cellular phosphatidylinositol, phosphatidylcholine, and phosphatidylethanolamine and an increase in the levels of corresponding lysophospholipids, but had no significant effect on sphingomyelin, phosphatidic acid, diglyceride, and other lipids. TNF-alpha did not evoke neutrophils prelabeled with [3H]lyso platelet activating factor to produce [3H]phosphatidylethanol, [3H]phosphatidic acid, or [3H]diglyceride in the presence of ethanol, indicating that phospholipases D and C were not activated. Treatment of the leukocytes with the cytokine had no effect on the activity of neutral and acidic sphingomyelinase. These data collectively provide evidence that TNF-alpha specifically induces the turnover of neutrophil phosphatidylinositol, phosphatidylcholine and phosphatidylethanolamine, which are enriched with 20:4(n-6) by the activation of phospholipase A2.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J. Ghosh and C. E. Myers Inhibition of arachidonate 5-lipoxygenase triggers massive apoptosis in human prostate cancer cells PNAS, October 27, 1998; 95(22): 13182 - 13187. [Abstract] [Full Text] [PDF]

				C. S. T. Hii, Z. H. Huang, A. Bilney, M. Costabile, A. W. Murray, D. A. Rathjen, C. J Der, and A. Ferrante Stimulation of p38 Phosphorylation and Activity by Arachidonic Acid in HeLa Cells, HL60 Promyelocytic Leukemic Cells, and Human Neutrophils. EVIDENCE FOR CELL TYPE-SPECIFIC ACTIVATION OF MITOGEN-ACTIVATED PROTEIN KINASES J. Biol. Chem., July 24, 1998; 273(30): 19277 - 19282. [Abstract] [Full Text] [PDF]

				J. L. Carroll Jr., D. M. McCoy, S. E. McGowan, R. G. Salome, A. J. Ryan, and R. K. Mallampalli Pulmonary-specific expression of tumor necrosis factor-alpha alters surfactant lipid metabolism Am J Physiol Lung Cell Mol Physiol, April 1, 2002; 282(4): L735 - L742. [Abstract] [Full Text] [PDF]