HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bird, D. A. Articles by Hamilton, J. A. Search for Related Content PubMed PubMed Citation Articles by Bird, D. A. Articles by Hamilton, J. A. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 37, 1449-1458, Copyright © 1996 by Lipid Research, Inc.

ARTICLES

Binding of ethyl oleate to low density lipoprotein, phospholipid vesicles, and albumin: a 13C NMR study

DA Bird, M Laposata and JA Hamilton
Department of Pathology, Massachusetts General Hospital, Boston, USA.

Fatty acid ethyl esters (FAEE), esterification products of ethanol and fatty acids, have been implicated as mediators of ethanol-induced organ damage. After ethanol ingestion in humans, FAEE circulate in blood, bound to lipoproteins and albumin. We have analyzed the binding of ethyl (1-13C, 99%) oleate (EO) to small unilamellar phospholipid vesicles (SUV), human low density lipoprotein (LDL), and bovine serum albumin (BSA) by 13C-NMR spectroscopy. Binding of < or = 25 mol% EO to SUV yielded a single EO carbonyl peak (172.6-172.9 ppm) downfield from that of EO oil (171.9 ppm). Thus, the carbonyl forms hydrogen bonds with water in the SUV aqueous interface. At 30 mol% EO in SUV, a second EO carbonyl peak appeared, indicating a limit in FAEE solubility in SUV. Addition of EO to isolated human LDL yielded a peak at 171.9 ppm, suggesting that the EO exists in an unhydrated environment, most likely the core of the lipoprotein. This binding was also observed using high levels of EO added to human serum. The addition of EO dissolved in ethanol or as an oil to a solution of BSA yielded no visible EO peak, whereas addition of (1-13C, 99%) oleic acid resulted in several narrow peaks, demonstrating a much greater affinity of BSA for oleic acid than for EO. Bidirectional transfer of EO between LDL and SUV was observed and was 85% complete within 30 min. There was no measurable transfer of EO from LDL or SUV to albumin. The weak binding of EO to albumin will result in increased transport of EO by lipoproteins as plasma levels of EO increase.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				C. A. BEST, M. LAPOSATA, V. G. PROIOS, and Z. M. SZCZEPIORKOWSKI METHOD TO ASSESS FATTY ACID ETHYL ESTER BINDING TO ALBUMIN Alcohol Alcohol., May 1, 2006; 41(3): 240 - 246. [Abstract] [Full Text] [PDF]

				D. Chan, B. Knie, R. Boskovic, and G. Koren Placental Handling of Fatty Acid Ethyl Esters: Perfusion and Subcellular Studies J. Pharmacol. Exp. Ther., July 1, 2004; 310(1): 75 - 82. [Abstract] [Full Text] [PDF]

				C. A. Best, J. E. Cluette-Brown, M. Teruya, A. Teruya, and M. Laposata Red blood cell fatty acid ethyl esters: a significant component of fatty acid ethyl esters in the blood J. Lipid Res., March 1, 2003; 44(3): 612 - 620. [Abstract] [Full Text] [PDF]

				A. Kabakibi, C. R. Morse, and M. Laposata Fatty acid ethyl esters and HepG2 cells: intracellular synthesis and release from the cells J. Lipid Res., August 1, 1998; 39(8): 1568 - 1582. [Abstract] [Full Text]