HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Murata, Y. Articles by Kasuga, M. Search for Related Content PubMed PubMed Citation Articles by Murata, Y. Articles by Kasuga, M. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 37, 1616-1622, Copyright © 1996 by Lipid Research, Inc.

ARTICLES

Cloning of rabbit LCAT cDNA: increase in LCAT mRNA abundance in the liver of cholesterol-fed rabbits

Y Murata, E Maeda, G Yoshino and M Kasuga
Second Department of Internal Medicine, Kobe University School of Medicine, Japan.

Structure of rabbit lecithin:cholesterol acyltransferase (LCAT) and molecular basis for the effects of cholesterol feeding on LCAT expression were investigated by cloning and sequencing LCAT cDNA from rabbit. The rabbit and human sequences are 91% identical at the nucleotide level and 93% identical at the amino acid level. The interfacial substrate active site, asparagine-linked glycosylation sites, and sites at which rare mutations cause human familial LCAT deficiency are all highly conserved in the rabbit protein. The apparent molecular mass of rabbit LCAT, as determined by immunoblot analysis, was approximately equal to that of human LCAT. Rabbits showed 2.6- and 5.5-fold increases in serum LCAT activity 3 and 6 weeks, respectively, after switching to a cholesterol-enriched diet. Northern blot analysis revealed that the abundance of LCAT mRNA in liver increased 1.6- and 2.8-fold after 3 and 6 weeks, respectively, of cholesterol feeding. The marked temporal relation between the increase in serum LCAT activity and the liver LCAT mRNA abundance suggest that the regulation of LCAT activity in vivo may be primarily determined by changes in the amount of LCAT mRNA.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M A. Zulet, A. Barber, H. Garcin, P. Higueret, and J. A. Martinez Alterations in Carbohydrate and Lipid Metabolism Induced by a Diet Rich in Coconut Oil and Cholesterol in a Rat Model J. Am. Coll. Nutr., February 1, 1999; 18(1): 36 - 42. [Abstract] [Full Text] [PDF]