Journal of Lipid Research, Vol 37, 1646-1654, Copyright © 1996 by Lipid Research, Inc.
Binding affinity and particle size of LDL in subjects with moderate hypercholesterolemia: relationship with in vivo LDL metabolism
K Yamane, S Kataoka, NA Le, M Paidi, WJ Howard, JS Hannah and BV Howard
Medlantic Research Institute, Washington, DC 20010, USA.
The aim of this study was to examine relationships between low density
lipoprotein (LDL) metabolism, in vitro binding, and particle size. Twenty
four study subjects, 17 men and 7 women, had elevated plasma total
cholesterol (TC), ranging from 174 to 232 mg/dl, and LDL cholesterol (LDLC)
ranging from 113 to 195 mg/dl after 12 weeks on a Step I diet. The
fractional clearance rate (FCR) for LDL ranged from 0.233 to 0.619
pools/day (0.366 +/- 0.021) and was significantly correlated with plasma
triglycerides (TG) (P < 0.05). Although there was no relation between
FCR and binding in the study group as a whole, those subjects with an FCR
within the normal range (< 0.45, n = 20), showed a significant negative
correlation between FCR and the KD for LDL binding, (r = 0.52). A subset of
four subjects with an elevated FCR (> 0.45) had higher production rate
(PR) (P < 0.005) and a significant positive correlation between the KD
for LDL binding and FCR (P < 0.05). LDL size varied from 240.0 to 265.8
A and was significantly inversely correlated with plasma TG (P < 0.001)
but there was no relation between LDL size and metabolism or binding
affinity. Thus, there appears to be a correlation between binding affinity
and clearance for subjects who had normal LDL production and clearance
rates. On the other hand, mechanisms other than binding affinity appear to
influence clearance in subjects with elevated rates of production and
clearance.
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