HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Frolov, A. Articles by Schroeder, F. Search for Related Content PubMed PubMed Citation Articles by Frolov, A. Articles by Schroeder, F. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 37, 1862-1874, Copyright © 1996 by Lipid Research, Inc.

ARTICLES

Fibroblast membrane sterol kinetic domains: modulation by sterol carrier protein-2 and liver fatty acid binding protein

A Frolov, JK Woodford, EJ Murphy, JT Billheimer and F Schroeder
Department of Physiology and Pharmacology, Texas A & M University, TVMC, College Station 77843-4466, USA.

The mechanism(s) of intracellular sterol trafficking among subcellular organelle membranes is not well understood. Relative contributions of vesicular, sterol carrier protein, and membrane sterol domain pathways are not resolved. A sterol kinetic assay was used to resolve multiple sterol domains in microsome (MICRO), mitochondria (MITO), and plasma (PM) membrane: exchangeable, 20-40% of total; non-exchangeable, 60-80% of total. Spontaneous sterol transfer between dissimilar donor and acceptor membranes was vectorial and depended both on acceptor and donor membrane properties. For example, sterol transfer from PM to MICRO or to MITO, or from MICRO to MITO was 3- to 5-fold slower as compared to sterol movement in the opposite direction. Sterol carrier protein-2 (SCP-2) stimulated sterol transfer in most donor/acceptor membrane combinations by decreasing exchange half-time but not domain size. SCP-2 enhanced sterol transfer selectively: PM-MICRO (12-fold); MITO-MITO, MICRO-MICRO, MICRO-PM (3-fold); PM-PM (1.4-fold); PM-MITO, MICRO-MITO (no effect). Thus, SCP-2-mediated sterol movement was vectorial and not necessarily down a membrane sterol concentration gradient. In contrast, liver fatty acid binding protein (L-FABP) revealed a modest (2-fold) stimulatory effect on sterol transfer only between PM-MITO and MICRO-MICRO. In conclusion, in vitro studies of sterol transfer among isolated subcellular membranes provided kinetic evidence for sterol domains in microsomes and mitochondria as well as plasma membranes. Furthermore, both spontaneous and protein-mediated sterol transfer appeared vectorial and selective in nature.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				G. G. Martin, B. P. Atshaves, A. L. McIntosh, J. T. Mackie, A. B. Kier, and F. Schroeder Liver fatty acid binding protein gene ablation potentiates hepatic cholesterol accumulation in cholesterol-fed female mice Am J Physiol Gastrointest Liver Physiol, January 1, 2006; 290(1): G36 - G48. [Abstract] [Full Text] [PDF]

				M. Fuchs, A. Hafer, C. Munch, F. Kannenberg, S. Teichmann, J. Scheibner, E. F. Stange, and U. Seedorf Disruption of the Sterol Carrier Protein 2 Gene in Mice Impairs Biliary Lipid and Hepatic Cholesterol Metabolism J. Biol. Chem., December 14, 2001; 276(51): 48058 - 48065. [Abstract] [Full Text] [PDF]

				F. Schroeder, A. M. Gallegos, B. P. Atshaves, S. M. Storey, A. L. McIntosh, A. D. Petrescu, H. Huang, O. Starodub, H. Chao, H. Yang, et al. Recent Advances in Membrane Microdomains: Rafts, Caveolae, and Intracellular Cholesterol Trafficking Experimental Biology and Medicine, November 1, 2001; 226(10): 873 - 890. [Abstract] [Full Text] [PDF]

				O. Starodub, C. A. Jolly, B. P. Atshaves, J. B. Roths, E. J. Murphy, A. B. Kier, and F. Schroeder Sterol carrier protein-2 localization in endoplasmic reticulum and role in phospholipid formation Am J Physiol Cell Physiol, October 1, 2000; 279(4): C1259 - C1269. [Abstract] [Full Text] [PDF]

				E. J. Murphy, T. Stiles, and F. Schroeder Sterol carrier protein-2 expression alters phospholipid content and fatty acyl composition in L-cell fibroblasts J. Lipid Res., May 1, 2000; 41(5): 788 - 796. [Abstract] [Full Text]

				A. Frolov, A. Petrescu, B. P. Atshaves, P. T. C. So, E. Gratton, G. Serrero, and F. Schroeder High Density Lipoprotein-mediated Cholesterol Uptake and Targeting to Lipid Droplets in Intact L-cell Fibroblasts. A SINGLE- AND MULTIPHOTON FLUORESCENCE APPROACH J. Biol. Chem., April 21, 2000; 275(17): 12769 - 12780. [Abstract] [Full Text] [PDF]

				K Boesze-Battaglia and R. Schimmel Collagen-stimulated unidirectional translocation of cholesterol in human platelet membranes J. Exp. Biol., January 2, 1999; 202(4): 453 - 460. [Abstract] [PDF]

				A. Frolov, T.-H. Cho, J. T. Billheimer, and F. Schroeder Sterol Carrier Protein-2, a New Fatty Acyl Coenzyme A-binding Protein J. Biol. Chem., December 13, 1996; 271(50): 31878 - 31884. [Abstract] [Full Text] [PDF]

				F. Schroeder, A. Frolov, O. Starodub, B. B. Atshaves, W. Russell, A. Petrescu, H. Huang, A. M. Gallegos, A. McIntosh, D. Tahotna, et al. Pro-sterol Carrier Protein-2. ROLE OF THE N-TERMINAL PRESEQUENCE IN STRUCTURE, FUNCTION, AND PEROXISOMAL TARGETING J. Biol. Chem., August 11, 2000; 275(33): 25547 - 25555. [Abstract] [Full Text] [PDF]

				A. D. Petrescu, A. M. Gallegos, Y. Okamura, J. F. Strauss III, and F. Schroeder Steroidogenic Acute Regulatory Protein Binds Cholesterol and Modulates Mitochondrial Membrane Sterol Domain Dynamics J. Biol. Chem., September 28, 2001; 276(40): 36970 - 36982. [Abstract] [Full Text] [PDF]

				M. Hao, S. X. Lin, O. J. Karylowski, D. Wustner, T. E. McGraw, and F. R. Maxfield Vesicular and Non-vesicular Sterol Transport in Living Cells. THE ENDOCYTIC RECYCLING COMPARTMENT IS A MAJOR STEROL STORAGE ORGANELLE J. Biol. Chem., January 4, 2002; 277(1): 609 - 617. [Abstract] [Full Text] [PDF]